Hematoporphyrin can inhibit the metabolism and growth of embryonic chicken cartilage in vitro

Vassilopoulou‐Sellin, Rena; Oyedeji, Caroline O.

doi:10.1016/0026-0495(90)90053-f

Cited by 1 publication

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References 21 publications

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“…Thus, in chondrocyte cell cultures, bilirubin-induced inhibition appears to be only partially reversible; this observation is at variance with our earlier findings using cartilage organ but consistent with the pattern we have found with other tetrapyrroles. (5)…”

Section: Reversibility Of Bjlirubin Effect On Chondrocyte Proliferationmentioning

confidence: 99%

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

Vassilopoulou‐Sellin

Rey-Bear

Oyedeji

1990

Journal of Bone and Mineral Research

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Elevated levels of bilirubin and other tetrapyrroles are common to a number of chronic hematologic and liver diseases that can result in growth failure. This report establishes a cellular model system for the study of these endogenous growth inhibitors. Primary chondrocyte cultures were prepared from embryonic chick cartilage; cells were incubated (0.3 X 10(5) cells per plate) in tissue culture medium containing 10% fetal bovine serum (FBS) with or without added bilirubin, 0.01-0.10 mM. Bilirubin caused profound, dose-dependent inhibition of chondrocyte proliferation: after 7 days, control incubations contained 11.45 +/- 2.0 X 10(5) cells per plate versus 4.92 +/- 0.55 X 10(5) cells per plate for wells with added 0.01 mM bilirubin and 1.76 +/- 0.30 X 10(5) for cultures with added 0.05 mM bilirubin. In chondrocytes cultured for 3 days, the addition of 0.05 mM bilirubin was associated with inhibition of [3H]thymidine incorporation into DNA (47 +/- 5% of control), [3H]uridine incorporation into RNA (11 +/- 0.05% of control), and [14C]leucine incorporation into proteins (16 +/- 1% of control). The inhibition of chondrocyte proliferation induced by a range of bilirubin concentrations (0.01-0.10 mM) was in no way attenuated by the addition of physiologic concentrations of albumin (4 g/dl). After 3 days in media containing bilirubin or heme (at equimolar concentrations), chondrocytes were subsequently incubated in FBS alone for an additional 3 days; only partial reversal of the bilirubin (or heme)-induced inhibition was then observed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Reversibility Of Bjlirubin Effect On Chondrocyte Proliferationmentioning

confidence: 99%

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

Vassilopoulou‐Sellin

Rey-Bear

Oyedeji

1990

Journal of Bone and Mineral Research

Self Cite

View full text Add to dashboard Cite

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Hematoporphyrin can inhibit the metabolism and growth of embryonic chicken cartilage in vitro

Cited by 1 publication

References 21 publications

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

Bilirubin as an inhibitor of cartilage metabolism: Effect on avian chondrocyte proliferation in cell culture

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