Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells

Matsushima, Miyoko; Takagi, Kenzo; Ogawa, Miyuki; Hirose, Etsuko; Ota, Yui; Abe, Fumie; Baba, Kenji; Hasegawa, Takaaki; Hasegawa, Yoshinori; Kawabe, Tsutomu

doi:10.1007/s00011-009-0039-1

Cited by 38 publications

(29 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14,15 In addition, HO-1 exerts its effects on multiple immune cells, such as promoting maturation, differentiation and function of dendritic cells, 16,17 inhibiting the antigen-presenting function of dendritic cells, 18 inducing tolerance of dendritic cells 19 and suppressing degranulation of mast cells. 20,21 In previous studies, HO-1 was found to enhance the function of CD4 + CD25 + regulatory T cells. 13,22 Recent studies have shown that the expression of HO-1 is significantly up-regulated in T helper type 2…”

Section: Introductionmentioning

confidence: 91%

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Zhong¹,

et al. 2016

Immunology

View full text Add to dashboard Cite

SummaryThe anti-inflammatory role of heme oxygenase-1 (HO-1) has been studied extensively in many disease models including asthma. Many cell types are anti-inflammatory targets of HO-1, such as dendritic cells and regulatory T cells. In contrast to previous reports that HO-1 had limited effects on basophils, which participate in T helper type 2 immune responses and antigeninduced allergic airway inflammation, we demonstrated in this study, for the first time, that the up-regulation of HO-1 significantly suppressed the maturation of mouse basophils, decreased the expression of CD40, CD80, MHC-II and activation marker CD200R on basophils, blocked DQ-ovalbumin uptake and promoted basophil apoptosis both in vitro and in vivo, leading to the inhibition of T helper type 2 polarization. These effects of HO-1 were mimicked by exogenous carbon monoxide, which is one of the catalytic products of HO-1. Furthermore, adoptive transfer of HO-1-modified basophils reduced ovalbumin-induced allergic airway inflammation. The above effects of HO-1 can be reversed by the HO-1 inhibitor Sn-protoporphyrin IX. Moreover, conditional depletion of basophils accompanying hemin treatment further attenuated airway inflammation compared with the hemin group, indicating that the protective role of HO-1 may involve multiple immune cells. Collectively, our findings demonstrated that HO-1 exerted its anti-inflammatory function through suppression of basophil maturation and activation, but promotion of basophil apoptosis, providing a possible novel therapeutic target in allergic asthma.

show abstract

Section: Introductionmentioning

confidence: 91%

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Zhong¹,

et al. 2016

Immunology

View full text Add to dashboard Cite

show abstract

“…Naringenin (flavonones) suppressed the activation of 1-4, 6, and 8 pathways and the downstream signal transducer of 2 and 12 [34,46,47] and induced production of 13 [48]. Quercetin (flavonols) modulated 1-2 [49] and 12-13 pathways [32,50], reduced the expression of 14 and 16 [32,51], inhibited 11 [52] degradation and attenuated the activation of points 3-10 [32,53]. Butein (chalcone) inhibited 11 degradation [54] and 1-2, 4-6, and 12 activation [54,55].…”

Section: Effect Of Flavonoids On the Production Of Lipid Mediatorsmentioning

confidence: 99%

Flavonoids as Anti-Inflammatory and Analgesic Drugs: Mechanisms of Action and Perspectives in the Development of Pharmaceutical Forms

Verri

Vicentini

Baracat

et al. 2012

Studies in Natural Products Chemistry

117

View full text Add to dashboard Cite

“…Bilirubin and biliverdin, the metabolites of HO-1, have been reported to exhibit immunomoduratory effects including the inhibition of NF-κB activity [25,26] . We also reported that quercetin increased the levels of bilirubin in mast cells, suggesting that bilirubin and by quercetin-induced HO-1 expression reflected attenuation of NF-κB activity [15] . However, HO-1 has been reported to inhibit NF-κB activity without modulating the nuclear translocation in endothelial cells [27] .…”

Section: Discussionmentioning

confidence: 91%

“…We previously reported that quercetin exerted cytoprotective effects via Nrf2-HO-1 pathway in mast cells, fibroblasts, and epithelial cells [15][16][17] . We next investigated whether quercetin was able to induce HO-1 in AMJ2-C11 cells.…”

Section: Modulation Of Ho-1 and Nqo1 Expression And Nrf2 Translocatiomentioning

confidence: 99%

“…These compounds exhibit anti-viral, antioxidant, anti-allergic, and anti-inflammatory activities [14] . Our previous studies on quercetin, one of the most common flavonoids, indicated that quercetin exerts cytoprotective effects via the heme oxygenase (HO)-1-dependent pathway in mast cells, fibroblasts, and alveolar epithelial cells [15][16][17] . HO-1 is a stress-inducible enzyme that exhibits cytoprotective effects against oxidative stress, a role mediated through the induction of the antioxidant activities of biliverdin and its metabolite, bilirubin, and by induction of carbon monoxide, which has anti-inflammatory effects [18] .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Suppressive Effect of Quercetin on Toll-Like Receptor 7-Mediated Activation in Alveolar Macrophages

Yasui

Matsushima²,

Omura³

et al. 2015

Pharmacology

Self Cite

View full text Add to dashboard Cite

Respiratory viral infections that cause chronic airway and lung disease can result in the activation of the innate immune response. Alveolar macrophages (AMs), one of the first lines of defense in the lung, are abundantly located in alveoli and the respiratory tract. Flavonoids found in fruits and vegetables exhibit cytoprotective effects on various cell types. In this study, we investigated the effect of quercetin on activation of AMs that had been exposed to imiquimod, a ligand of Toll-like receptor (TLR) 7. In both a mouse AM cell line (AMJ2-C11 cells) and mouse bronchoalveolar fluid cells, we demonstrated that quercetin attenuated TLR7-induced the expression of TNF-α and IL-6. In AMJ2-C11 cells, quercetin also attenuated the TLR7-induced CD40 expression; attenuated the translocation of p65; induced translocation of Nrf2 from cytosol to nucleus; and induced heme oxygenase (HO)-1 expression. Notably, tin protoporphyrin IX (SnPP), an inhibitor of HO-1, also attenuated TLR7-induced transcription of the TNF-α and IL-6 genes, suggesting that the effect of quercetin is mediated by HO-1. These results suggest that dietary supplementation with quercetin may have efficacy in the treatment of respiratory viral infection.

show abstract

Heme oxygenase-1 mediates the anti-allergic actions of quercetin in rodent mast cells

Abstract: These results strongly suggest that quercetin exerted anti-allergic actions via activation of Nrf2-HO-1 pathway.

Cited by 38 publications

References 41 publications

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Heme oxygenase‐1 inhibits basophil maturation and activation but promotes its apoptosis in T helper type 2‐mediated allergic airway inflammation

Flavonoids as Anti-Inflammatory and Analgesic Drugs: Mechanisms of Action and Perspectives in the Development of Pharmaceutical Forms

The Suppressive Effect of Quercetin on Toll-Like Receptor 7-Mediated Activation in Alveolar Macrophages

Contact Info

Product

Resources

About