Heme oxygenase-1 protects against inflammatory and apoptosis induced by hemeproteins in Ctenopharyngodon Idellus kidney cells

Lu, Zhijie; Zhang, Menglan; Tang, Meizhen; Li, Yanan; Shi, Fei; Zhao, Lijuan; Li, Jun; Lin, Li; Qin, Zhendong

doi:10.1016/j.aquaculture.2021.737266

Cited by 5 publications

(4 citation statements)

References 70 publications

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“…Our study revealed that the PHZ-induced hemolysis significantly increased the expression of HO-1 both in head and middle kidney, which indicated that the HO-1 in grass carp kidney plays a vital role affording protection against cell-free heme, thus exerting salutary effects against oxidative damage. This result is consistent with our previous study in vitro ( 41 ). In this study, we have demonstrated that the PHZ-induced hemolysis resulted in the accumulation of excess intracellular iron and tissue damage, destroying systemic iron homeostasis.…”

Section: Discussionsupporting

confidence: 94%

“…In a human podocyte model, during intravascular hemolysis, podocytes take up Hb, promoting oxidative stress, podocyte dysfunction, and apoptosis (18). In our previous in vitro study, high levels of Hb caused apoptosis in CIK cells (41). Here, we also examined the effect of hemolysis on apoptosis in both head and middle kidney through the TUNEL assay.…”

Section: Discussionmentioning

confidence: 96%

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The Oxidative Injury of Extracellular Hemoglobin Is Associated With Reactive Oxygen Species Generation of Grass Carp (Ctenopharyngodon idella)

Qin

Yang

et al. 2022

Front. Immunol.

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Intravascular hemolysis is a fundamental feature of hemorrhagic venereal infection or tissue and releases the endogenous damage-associated molecular pattern hemoglobin (Hb) into the plasma or tissues, which results in systemic inflammation, vasomotor dysfunction, thrombophilia, and proliferative vasculopathy. However, how the cytotoxic Hb affects the tissues of grass carp remains unclear. Here, we established a hemolysis model in grass carp by injecting phenylhydrazine (PHZ). The data revealed that the PHZ-induced hemolysis increased the content of Hb and activated the antioxidant system in plasma. The histopathology analysis data showed that the PHZ-induced hemolysis increased the accumulation of Hb and iron both in the head and middle kidney. The results of quantitative real-time PCR (qRT-PCR) detection suggested that the hemolysis upregulated the expressions of iron metabolism-related genes. In addition, the immunofluorescence and immunohistochemistry data revealed that the hemolysis caused an obvious deposition of collagen fiber, malondialdehyde (MDA), and 4-hydroxynonenal (4-HNE) accumulation and increased the content of oxidative-related enzymes such as β-galactosidase (β-GAL), lipid peroxide (LPO), and MDA in both the head and middle kidney. Furthermore, the PHZ-induced hemolysis significantly increased the production of reactive oxygen species (ROS), which resulted in apoptosis and modulated the expressions of cytokine-related genes. Taken together, excess of Hb released from hemolysis caused tissue oxidative damage, which may be associated with ROS and inflammation generation.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 96%

The Oxidative Injury of Extracellular Hemoglobin Is Associated With Reactive Oxygen Species Generation of Grass Carp (Ctenopharyngodon idella)

Qin

Yang

et al. 2022

Front. Immunol.

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“…Such mRNA upregulation led not only to remarkably elevated NQO1 but also HO-1 and Keap1 protein levels, as revealed by Figure . Studies have shown that HO-1 overexpression can significantly induce Keap1 and then eliminate ROS produced in cells to play an anti-inflammatory role. , Activation of the intracellular NF-κB pathway negatively regulates the Nrf2 pathway . Therefore, we examined Keap1 and Nrf2 as targets of poricoic acid GM ( 39 ).…”

Section: Resultsmentioning

confidence: 99%

New Lanostane-Type Triterpenes with Anti-Inflammatory Activity from the Epidermis of Wolfiporia cocos

Bao

Long

Wang

et al. 2022

J. Agric. Food Chem.

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A chemical study on the epidermis of cultivated edible mushroom Wolfiporia cocos resulted in the isolation and identification of 46 lanostane triterpenoids, containing 17 new compounds (1–17). An experimental determination of their anti-inflammatory activity showed that poricoic acid GM (39) most strongly inhibited NO production in LPS-induced RAW264.7 murine macrophages with an IC50 value at 9.73 μM. Furthermore, poricoic acid GM induced HO-1 protein expression and inhibited iNOS and COX2 protein expression as well as the release of PGE2, IL-1β, IL-6, TNF-α, and reactive oxygen species (ROS) in LPS-induced RAW264.7 cells. Mechanistically, poricoic acid GM suppressed the phosphorylation of the IκBα protein, which prevented NF-κB from entering the nucleus to lose transcriptional activity and inhibited the dissociation of Keap1 from Nrf2, thereby activating Nrf2 into the nucleus to regulate antioxidant genes. Furthermore, the MAPK signaling pathway may play a significant role in poricoic acid GM-induced elimination of inflammation. This work further confirms that lanostane triterpenoids are key ingredients responsible for the anti-inflammatory properties of the edible medicinal mushroom W. cocos.

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“…HO-1 is the rate limiting enzyme of heme catabolism. HO-1 and its metabolites have anti-inflammatory, anti-proliferative, antioxidant and anti-apoptotic effects in the process of maintaining intracellular homeostasis (Lu et al, 2022). Studies have shown that a small amount of H 2 O 2 can promote the slight or strong increase of HO-1, while higher H 2 O 2 concentration can significantly reduce the expression of HO-1 (Jia et al, 2019).…”

Section: Effects Of Transport Density On Antioxidant Indexes Of Whole...mentioning

confidence: 99%

Effect of Transport Density on Greater Amberjack (Seriola dumerili) Stress, Metabolism, Antioxidant Capacity and Immunity

Liu

et al. 2022

Front. Mar. Sci.

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This study sought to characterize the effect of density stress on greater amberjack (Seriola dumerili) survival to determine an optimal transport density. To achieve this, this experiment simulated the transport conditions of fish (body length: 4.09 ± 1.00 cm; weight: 0.9 ± 0.05 g) using closed oxygen transport at 5 different densities (D1 = 1.125 kg/m3, D2 = 2.25 kg/m3, D3 = 3.375 kg/m3, D4 = 4.5 kg/m3, and D5 = 6.75 kg/m3) for 8 hours, after which biochemical indicators, stress, metabolism, and antioxidant capacity were evaluated. After 8h, only the D1 and D3 groups exhibited survival rates above 90%. The pH of the water decreased with density, whereas the ammonia nitrogen and nitrite increased with density. Cortisol (COR) levels were not significantly different among all the groups, but tended to increase with increasing density. In this study, COR is a relatively stable index with the increase in density, but in D3 group, the change of COR will not cause the change of immune-related genes, so COR can inhibit the expression of immune genes within a certain density. The D3 density group exhibited the highest catalase (CAT) and glutathione peroxidase glutathione peroxidase (GSH‐PX) levels. In addition, the expression levels of immune related factor interleukin-1β (IL-1β), tumor necrosis factor (TNF-α), major histocompatibility complex (MHC-1) and nuclear factor kappa-B (NF-KB1) were the lowest in D3 density group. The density of fish during transportation significantly affects water quality, metabolism, and immunity. During transportation, assuming that the plastic bags are airtight to ensure aerobic conditions and that the transportation time is within 8 h, transport density should be maintained at approximately 3.375 kg/m3.

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Heme oxygenase-1 protects against inflammatory and apoptosis induced by hemeproteins in Ctenopharyngodon Idellus kidney cells

Cited by 5 publications

References 70 publications

The Oxidative Injury of Extracellular Hemoglobin Is Associated With Reactive Oxygen Species Generation of Grass Carp (Ctenopharyngodon idella)

The Oxidative Injury of Extracellular Hemoglobin Is Associated With Reactive Oxygen Species Generation of Grass Carp (Ctenopharyngodon idella)

New Lanostane-Type Triterpenes with Anti-Inflammatory Activity from the Epidermis of Wolfiporia cocos

Effect of Transport Density on Greater Amberjack (Seriola dumerili) Stress, Metabolism, Antioxidant Capacity and Immunity

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