Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica

Wb, Zeng; Wb, Chen; Qp, Yan; Gf, Lin; Yx, Qin

doi:10.4238/gmr.15028074

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…The presence of unbound metals in the cell must be strictly regulated since the imbalance in iron homeostasis can lead to the formation of ROS, mismetallation or other anomalies leading to the incorrect function of proteins. The relationship between hemerythrin and defense against oxidative stress was previously suggested in bacteria [36,37] and so was the role of hemerythrin-related proteins in iron homeostasis [38] or oxygen sensing [39]. One of the basic mechanisms of maintaining the proper intracellular level of metals is the regulation of their acquisition.…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 98%

Adaptive iron utilization compensates for the lack of an inducible uptake system in Naegleria fowleri and represents a potential target for therapeutic intervention

et al. 2020

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Naegleria fowleri is a single-cell organism living in warm freshwater that can become a deadly human pathogen known as a brain-eating amoeba. The condition caused by N. fowleri, primary amoebic meningoencephalitis, is usually a fatal infection of the brain with rapid and severe onset. Iron is a common element on earth and a crucial cofactor for all living organisms. However, its bioavailable form can be scarce in certain niches, where it becomes a factor that limits growth. To obtain iron, many pathogens use different machineries to exploit an iron-withholding strategy that has evolved in mammals and is important to host-parasite interactions. The present study demonstrates the importance of iron in the biology of N. fowleri and explores the plausibility of exploiting iron as a potential target for therapeutic intervention. We used different biochemical and analytical methods to explore the effect of decreased iron availability on the cellular processes of the amoeba. We show that, under iron starvation, nonessential, iron-dependent, mostly cytosolic pathways in N. fowleri are downregulated, while the metal is utilized in the mitochondria to maintain vital respiratory processes. Surprisingly, N. fowleri fails to respond to acute shortages of iron by inducing the reductive iron uptake system that seems to be the main iron-obtaining strategy of the parasite. Our findings suggest that iron restriction may be used to slow the progression of infection, which may make the difference between life and death for patients.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 98%

Adaptive iron utilization compensates for the lack of an inducible uptake system in Naegleria fowleri and represents a potential target for therapeutic intervention

et al. 2020

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“…Hemerythrin was initially described as a multimeric O 2 carrier-protein with a binuclear nonheme iron center and with a distribution that, at first, appeared to be limited to three phyla of marine invertebrates, Brachiopoda, Priapulida, and Annelida. 1 Over the past decade, however, hemerythrinlike proteins have been identified in many taxonomically distant groups including humans, 2-4 plants, 5 bacteria, [6][7][8][9][10] and archaea (PDB code: 2P0N). The function of these binuclear nonheme Fe-containing proteins (e.g., hemerythrin, myohemerytrin, bacteriohemerythrin) is tightly related to O 2 -binding and activation, [11][12][13] a trait indicative of the major evolutionary pressure exerted by atmospheric and oceanic oxygenation since the late Archaean Eon on the biosphere.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the primary structure of the Rv2633c hemerythrin-like catalase from Mycobacterium tuberculosis (Uniprot ID: P9WL59), 25 and hemerythrin homologs in Mycobacterium smegmatis (Uniprot IDs: A0QXI3, A0QV17, and A0R5J3); 26 Anabaena sp. strain PCC7120 (Uniprot ID: Q8YS92); 27 Aeromonas hydrophila (Uniprot ID: A0KMZ0); 10 Acidothermus cellulolyticus (Uniprot ID: A0LQU2); 28 Oryza sativa subsp. japonica (Uniprot IDs: V9G2Z0; and Q6AUD8) and Arabidopsis thaliana (Uniprot ID: Q8LPQ5), 5 suggests that these proteins also have differences in the first coordination-sphere of the iron centers, but there is no available confirmation from structural data.…”

Section: Introductionmentioning

confidence: 99%

Structure, function and evolution of the hemerythrin‐like domain superfamily

et al. 2018

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Hemerythrin-like proteins have generally been studied for their ability to reversibly bind oxygen through their binuclear nonheme iron centers. However, in recent years, it has become increasingly evident that some members of the hemerythrin-like superfamily also participate in many other biological processes. For instance, the binuclear nonheme iron site of YtfE, a hemerythrin-like protein involved in the repair of iron centers in Escherichia coli, catalyzes the reduction of nitric oxide to nitrous oxide, and the human F-box/LRR-repeat protein 5, which contains a hemerythrin-like domain, is involved in intracellular iron homeostasis. Furthermore, structural data on hemerythrin-like domains from two proteins of unknown function, PF0695 from Pyrococcus furiosus and NMB1532 from Neisseria meningitidis, show that the cation-binding sites, typical of hemerythrin, can be absent or be occupied by metal ions other than iron. To systematically investigate this functional and structural diversity of the hemerythrin-like superfamily, we have collected hemerythrin-like sequences from a database comprising fully sequenced proteomes and generated a cluster map based on their all-against-all pairwise sequence similarity. Our results show that the hemerythrin-like superfamily comprises a large number of protein families which can be classified into three broad groups on the basis of their cation-coordinating residues: (a) signal-transduction and oxygen-carrier hemerythrins (H-HxxxE-HxxxH-HxxxxD); (b) hemerythrin-like (H-HxxxE-H-HxxxE); and, (c) metazoan F-box proteins (H-HExxE-H-HxxxE). Interestingly, all but two hemerythrin-like families exhibit internal sequence and structural symmetry, suggesting that a duplication event may have led to the origin of the hemerythrin domain.

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Section: Resultsmentioning

confidence: 99%

“…Two of these genes, encoding for a hemerythrin and a catalase, were confidently found in the genomes of JF2480, Y567, and Y47, which are virulent against D. discoideum ( Table 2). Homologs of these two genes were already listed to be involved in the virulence of other bacteria [48,49]. Given the novelty of the host, it was relevant to investigate the genes unique to JF2480 to have clues about the genetic determinants that may be involved in its success of colonization.…”

Section: Resultsmentioning

confidence: 99%

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

et al. 2019

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Aeromonas salmonicida is a Gram-negative bacterium, known as a fish pathogen since its discovery. Although the species was initially considered psychrophilic, a mesophilic subspecies (pectinolytica) and many other mesophilic strains still not attributed to subspecies have been described in the last two decades. These mesophilic strains were sampled from various sources, including humans, and some of them are known to be pathogenic. In this study, we describe a strain, JF2480, which was isolated from the spleen, and also found the kidney and liver of a dead pied avocet (Recurvirostra avosetta), a type of migratory bird inhabiting aquatic environments. A core genome phylogenomic analysis suggests that JF2480 is taxonomically distant from other known A. salmonicida subspecies. The genome sequence confirms that the strain possesses key virulence genes that are present in the typical A. salmonicida psychrophilic subspecies, with the exception of the genes encoding the type three secretion system (T3SS). Bacterial virulence assays conducted on the surrogate host Dictyostelium discoideum amoeba confirmed that the strain is virulent despite the lack of T3SS. Bacterial growth curves showed that strain JF2480 grow well at 40 °C, the body temperature of the pied avocet, and even faster at 41 °C, compared to other mesophilic strains. Discovery of this strain further demonstrates the extent of the phylogenomic tree of this species. This study also suggests that A. salmonicida can infect a wider array of hosts than previously suspected and that we need to rethink the way we perceive A. salmonicida’s natural environment.

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Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica

Cited by 7 publications

References 32 publications

Adaptive iron utilization compensates for the lack of an inducible uptake system in Naegleria fowleri and represents a potential target for therapeutic intervention

Adaptive iron utilization compensates for the lack of an inducible uptake system in Naegleria fowleri and represents a potential target for therapeutic intervention

Structure, function and evolution of the hemerythrin‐like domain superfamily

A Mesophilic Aeromonas salmonicida Strain Isolated from an Unsuspected Host, the Migratory Bird Pied Avocet

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