Hemicellulolytic enzymes of the ligninolytic white‐rot fungus <i>phlebia radiata</i>. determination of enzyme activities

Rogalski, Jerzy; Hatakka, Annele; Longa, B.; Leonowicz, Andrzej

doi:10.1002/abio.370130110

Cited by 9 publications

(4 citation statements)

References 15 publications

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“…Furthermore, expression of certain CAZyme gene families such as various hemicellulases [156,157] and feruloyl esterases [158] may promote wood lignin reactivity and xylem cell wall accessibility for the fungal LMEs.…”

Section: Oxalic Acid and H 2 O 2 In Wood Decaymentioning

confidence: 99%

Lignin‐modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review

Lundell

Mäkelä

Hildén

2010

J. Basic Microbiol.

398

236

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Filamentous fungi owe powerful abilities for decomposition of the extensive plant material, lignocellulose, and thereby are indispensable for the Earth's carbon cycle, generation of soil humic matter and formation of soil fine structure. The filamentous wood-decaying fungi belong to the phyla Basidiomycota and Ascomycota, and are unique organisms specified to degradation of the xylem cell wall components (cellulose, hemicelluloses, lignins and extractives). The basidiomycetous wood-decaying fungi form brackets, caps or resupinaceous (corticioid) fruiting bodies when growing on wood for dissemination of their sexual basidiospores. In particular, the ability to decompose the aromatic lignin polymers in wood is mostly restricted to the white rot basidiomycetes. The white-rot decay of wood is possible due to secretion of organic acids, secondary metabolites, and oxidoreductive metalloenzymes, heme peroxidases and laccases, encoded by divergent gene families in these fungi. The brown rot basidiomycetes obviously depend more on a non-enzymatic strategy for decomposition of wood cellulose and modification of lignin. This review gives a current ecological, genomic, and protein functional and phylogenetic perspective of the wood and lignocellulose-decaying basidiomycetous fungi.

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Section: Oxalic Acid and H 2 O 2 In Wood Decaymentioning

confidence: 99%

Lignin‐modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review

Lundell

Mäkelä

Hildén

2010

J. Basic Microbiol.

398

236

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“…It has been reported that the glycosylation in fungal laccase plays a role in secretion, copper retention, susceptibility to proteolytic degradation, and thermal stability [49,50]. The growth conditions of fungi and their physiological states are responsible for the expression of different laccase isoenzymes, which are coded by gene families and differentially regulated [4,7,41,51,52]. In P. ostreatus, 12 possible genes encoding laccases have been reported and only described and characterized 7 isoenzymes laccase: lacc2 [47], lacc4 [53], lacc6 [54], lacc9 [55], lacc10 [56,57], lacc12 [58] and lacP83 [7].…”

Section: Laccasesmentioning

confidence: 99%

Characterization of the Solid-State and Liquid Fermentation for the Production of Laccases of Pleurotus ostreatus

Díaz‐Godínez¹,

Téllez‐Téllez²,

Sánchez³

et al. 2017

Fermentation Processes

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In this chapter, the activity and isoenzymes number of laccases of Pleurotus ostreatus grown in solid-state and liquid fermentations are reported. "n atypical behavior of this fungus with relation on enzyme production was observed, since the major laccase activity levels were observed in liquid fermentation, whereas the solid-state fermentation has been recognized as beter system for enzyme production.

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“…The presence of hydrolases in this study, as expressed in the secretome of fungi, supports this observation. Past research indicated that P. radiata, as well as other white rot fungi, can produce enzymes such as endo-1, 4-β-xylanase and ferulic acid esterase (Rogalski et al, 1993, Pérez et al, 2002, enabling them to efficiently dissolve hemicellulose linkages as well as break the bond between ferulic acid in the wheat straw and the lignin structure (Jung and Himmelsbach, 1989). Figure 2 shows that in the C NMR spectra, there is a significant amount of degradation across the whole spectrum.…”

Section: H and 13 C Nmr Analysismentioning

confidence: 99%

Effects of extracellular proteome on wheat straw pretreatment during solid-state fermentation of Phlebia radiata ATCC 64658

Bule

Chaudhary

Gao

et al. 2016

International Biodeterioration & Biodegradation

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Biological pretreatment of lignocellulosic biomass potentially offers a less energy intensive alternative to chemical pretreatment for the reduction of recalcitrance towards cellulolytic enzymes, but possesses some disadvantages, including a slow rate and loss of polysaccharides. This study characterizes the biodegradation of wheat straw by Phlebia radiata, a white rot fungus capable of secreting three major classes of ligninolytic enzymes: lignin peroxidase, manganese peroxidase, and laccase under natural conditions. We investigated the correlation between synergistic action of fungal enzymes and characteristics of the pretreated biomass. The results showed a sequential expression of enzymes over the course of a three-week pretreatment, with members of the peroxidase family being expressed in week one, followed by laccase expression starting in week two which continued until the course of pretreatment. This highlights the synergy of ligninolytic enzymes in the selective degradation pattern for wheat straw. 1 H-13 C HSQC NMR spectroscopy results demonstrated reduced amounts of syringyl (S) and hydroxyphenyl (H) lignin after pretreatment. Moreover, the reduction in H lignin was also seen in Pyrolysis-GC/MS and FT-IR results. This strongly suggests that this unique lignin modification pattern is associated with Phlebia radiata extracellular proteome, as expressed during the solid-state fermentation (pretreatment) of wheat straw.

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Hemicellulolytic enzymes of the ligninolytic white‐rot fungus phlebia radiata. determination of enzyme activities

Abstract: The ligninolytic fungus Phfebiu rudiutu growing in a low-nitrogen medium, with wheat bran as the sole carbon source, produces a wide spectrum of hemicellulolytic enzymes. During the growth of the fungal culture, some properties of these enzymes were investigated.

Cited by 9 publications

References 15 publications

Lignin‐modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review

Lignin‐modifying enzymes in filamentous basidiomycetes – ecological, functional and phylogenetic review

Characterization of the Solid-State and Liquid Fermentation for the Production of Laccases of Pleurotus ostreatus

Effects of extracellular proteome on wheat straw pretreatment during solid-state fermentation of Phlebia radiata ATCC 64658

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