Hemicellulosic Ethanol Production in Fluidized Bed Reactor from Sugar Cane Bagasse Hydrolysate: Interplay among Carrier Concentration and Aeration Rate

Antunes, Felipe Antônio Fernandes; Chandel, Anuj K.; Santos, Júlio César dos; Milessi, Thais S.; Peres, Guilherme; Silva, Sílvio Silvério da

doi:10.1021/acssuschemeng.7b01916

Cited by 15 publications

(6 citation statements)

References 34 publications

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“…However, the low sugar content led to a low ethanol titer. Antunes et al [40], using the yeast S. shehatae UFMG-HM 52.2 immobilized in Ca-alginate gel, performed five repeated batches with similar performance in a fluidized bed reactor using detoxified sugarcane bagasse hemicellulose hydrolysate (31 g/L xylose, 0.3 g/L glucose and 1.6 g/L acetic acid). However, the yield and productivity dropped significantly from the sixth cycle.…”

Section: Discussionmentioning

confidence: 99%

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

Milessi

Perez

Zangirolami

et al. 2020

Biotechnol Biofuels

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Background: The search for sustainable energy sources has become a worldwide issue, making the development of efficient biofuel production processes a priority. Immobilization of second-generation (2G) xylose-fermenting Saccharomyces cerevisiae strains is a promising approach to achieve economic viability of 2G bioethanol production from undetoxified hydrolysates through operation at high cell load and mitigation of inhibitor toxicity. In addition, the use of a fixed-bed reactor can contribute to establish an efficient process because of its distinct advantages, such as high conversion rate per weight of biocatalyst and reuse of biocatalyst. Results: This work assessed the influence of alginate entrapment on the tolerance of recombinant S. cerevisiae to acetic acid. Encapsulated GSE16-T18SI.1 (T18) yeast showed an outstanding performance in repeated batch fermentations with cell recycling in YPX medium supplemented with 8 g/L acetic acid (pH 5.2), achieving 10 cycles without significant loss of productivity. In the fixed-bed bioreactor, a high xylose fermentation rate with ethanol yield and productivity values of 0.38 g ethanol /g sugars and 5.7 g/L/h, respectively were achieved in fermentations using undetoxified sugarcane bagasse hemicellulose hydrolysate, with and without medium recirculation. Conclusions: The performance of recombinant strains developed for 2G ethanol production can be boosted strongly by cell immobilization in alginate gels. Yeast encapsulation allows conducting fermentations in repeated batch mode in fixed-bed bioreactors with high xylose assimilation rate and high ethanol productivity using undetoxified hemicellulose hydrolysate.

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Section: Discussionmentioning

confidence: 99%

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

Milessi

Perez

Zangirolami

et al. 2020

Biotechnol Biofuels

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“…In this figure, soy molasses with the studied concentrations showed to be promising for the production of the ACPase when subjected to the treatment process. The clarification protocols are also used to reduce toxic effects in detoxification processes commonly performed for partial removal of interferents [20,21].…”

Section: Resultsmentioning

confidence: 99%

Acid Phosphatase Immobilization and Production Study by Trichoderma spp. in Soybean Molasses

et al. 2022

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Acid phosphatase production was carried out by Trichoderma spp. using treated soybean molasses at different concentrations. Results showed higher acid phosphatase activity when the soybean molasses concentration increased for the microorganisms Trichoderma harzianum and Trichoderma reesei. The acid phosphatase activity performance of Trichoderma asperellum was also evaluated. In general, the polyethyleneimine glyoxyl agarose support showed much better performance and final recovery of activity than DEAE Toyopearl 650s.

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“…It has the advantages of short fermentation time, controllable working environment and a high degree of mechanization when compared with the traditional fermentation method. In recent years, with the in-depth study of SSF bioreactor, it has been applied in the cultivation of animal and plant cell lines, and production of enzymes (14), ethanol (15), organic acids, pigments (16), and beer.…”

Section: Introductionmentioning

confidence: 99%

Changes in vinegar quality and microbial dynamics during fermentation using a self-designed drum-type bioreactor

et al. 2023

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The bioreactor based on solid-state fermentation technology has been developed for vinegar production, standardization of fermentation process and stabilization of vinegar quality. The microbial community diversity, and volatile compounds of six cultivars of vinegar samples fermented in a self-designed solid-state fermentation bioreactors were investigated using Illumina MiSeq platform and gas chromatography mass spectrometry (GC-MS) technology. The correlations between the richness and diversity of microbiota and volatile profiles, organic acids, as well as physicochemical indicators were explored by R software with the coplot package. The findings indicated that Acetobacter, norank-c-Cyanobacteria, and Weissella played key roles during fermentation process. Norank-f-Actinopolyporaceae, norank-c-Cyanobacteria, Pediococcus, and Microbacterium had significant correlations with the physicochemical characteristics. The most common bacterial species were associated with a citric acid content, whereas the least number of bacterial species correlated with malic acid content. Findings could be helpful for the bioreactor optimization, and thus reaching the level of pilot scale and industrialization.

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Hemicellulosic Ethanol Production in Fluidized Bed Reactor from Sugar Cane Bagasse Hydrolysate: Interplay among Carrier Concentration and Aeration Rate

Cited by 15 publications

References 34 publications

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

Repeated batches as a strategy for high 2G ethanol production from undetoxified hemicellulose hydrolysate using immobilized cells of recombinant Saccharomyces cerevisiae in a fixed-bed reactor

Acid Phosphatase Immobilization and Production Study by Trichoderma spp. in Soybean Molasses

Changes in vinegar quality and microbial dynamics during fermentation using a self-designed drum-type bioreactor

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