Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells

Jahn, Markus; Shukoor, Ibrahim; Tremel, Wolfgang; Wolfrum, Uwe; Kolb, Ute; Nawroth, Thomas; Langguth, Peter

doi:10.1111/j.2042-7158.2011.01356.x

Cited by 16 publications

(9 citation statements)

References 45 publications

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“…There are many articles about uptake and translocation of NPs through monolayers of Caco-2 cells (Jahn et al, 2011;Zariwala et al, 2013) or co-culture that produce mucus (Kenzaoui et al, 2012). Brun et al (2014) described titanium oxide NPs uptake in Caco-2 mono and co-cultures and in mice, studying the PC composition of NPs incubated in 10% serum proteins and subjected to simulated digestion.…”

Section: Translocation and Uptake Of Pc Complexesmentioning

confidence: 98%

“…This is mainly due to the experimental difficulty in isolating PC NPs from such a dynamic environment without modifying their properties. For example, Jahn et al (2011) studied the uptake of hemin-coupled iron hydroxide NPs on Caco-2 cells and they speculated a strong effect due to the colloidal stability of such NPs in the medium, but they did not perform any stability study. Pereira et al (2013) found that Fe(III)-oxo hydroxide NPs forms ferrihydrite-like complexes after a simulated in vitro digestion.…”

Section: Stability Of Nps In Simulated Gastrointestinal Fluidsmentioning

confidence: 98%

“…Multiple endocytic energy-dependent routes (e.g. clathrin, calveolin mediated endocytosis) can be speculated, given the differences in size, density and corona composition of the PC complexes (Bannunah et al, 2014;Jahn et al, 2011). More images for digested NPs interacting with the cell are provided in the SI, Figs.…”

Section: Translocation and Uptake Of Pc Complexesmentioning

confidence: 98%

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Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Silvio

Rigby

Bajka

et al. 2016

The International Journal of Biochemistry & Cell Biology

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Nanoparticles (NPs) in biological fluids immediately interact with proteins forming a biomolecular corona (PC) that imparts their biological identity. While several studies on the formation of the PC in human plasma have been reported, the PC of orally administrated NPs has been less investigated, mostly in the presence of a food matrix. In fact, food matrixes when digested are subject of several dynamic changes that will certainly affect the PC formed on the NPs. The lack of studies on this topic is clearly related to the difficulty in isolating representative PC NPs from such a complex environment. In this work magnetite NPs were added to in vitro simulated digestion simultaneously with bread and PC NPs were isolated after gastric and duodenal phases by sucrose gradient ultracentrifugation (UC). The PC NPs were characterized in terms of size and protein composition. Translocation studies were then performed on Caco-2 monolayers in a serum free environment and cell morphology was characterized by confocal microscopy. PC NPs isolated from gastric and duodenal phases were different in size, surface charge and protein corona composition. NP cellular uptake was enhanced by the digestive PC inducing morphology changes in the cell monolayer. Overall, in this work we were able to isolate PC NPs from digested fluids in the presence of a food matrix and study their biological response on Caco-2 cells.

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Section: Translocation and Uptake Of Pc Complexesmentioning

confidence: 98%

Section: Stability Of Nps In Simulated Gastrointestinal Fluidsmentioning

confidence: 98%

Section: Translocation and Uptake Of Pc Complexesmentioning

confidence: 98%

See 1 more Smart Citation

Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Silvio

Rigby

Bajka

et al. 2016

The International Journal of Biochemistry & Cell Biology

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“…Caco-2 cells confirmed intestinal absorption of well characterized hemin-coupled iron (III)-hydroxide NPs and of chitosan coated, iron loaded solid lipid NPs, as possible alternatives to oral ferrous salt supplements in iron mineral deficiency (Jahn et al, 2011;Zariwala et al, 2013). The permeability of 40 nm titanium dioxide NMs was also determined with Caco-2 cells using a dual chamber approach (Koeneman et al, 2010).…”

Section: In Vitro Cell Culture Modelsmentioning

confidence: 99%

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Venema²,

et al. 2014

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Engineered metal/mineral, lipid and biochemical macromolecule nanomaterials (NMs) have potential applications in food. Methodologies for the assessment of NM digestion and bioavailability in the gastrointestinal tract are nascent and require refinement. A working group was tasked by the International Life Sciences Institute NanoRelease Food Additive project to review existing models of the gastrointestinal tract in health and disease, and the utility of these models for the assessment of the uptake of NMs intended for food. Gastrointestinal digestion and absorption could be addressed in a tiered approach using in silico computational models, in vitro non-cellular fluid systems and in vitro cell culture models, after which the necessity of ex vivo organ culture and in vivo animal studies can be considered. Examples of NM quantification in gastrointestinal tract fluids and tissues are emerging; however, few standardized analytical techniques are available. Coupling of these techniques to gastrointestinal models, along with further standardization, will further strengthen methodologies for risk assessment.

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“…9 Briefly, high-resolution transmission electron microscopy operated at accelerating voltages of 200 KV were used to determine morphology of NPs. DLS was applied to analyze the hydrodynamic diameter and zeta potentials on a ZetaPALS Analyzer (Brookhaven, NY).…”

Section: Materials and Methods Synthesis Of Fe 3 O 4 Nanoparticlesmentioning

confidence: 99%

Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the Beclin 1/Bcl-2/VPS34 complex

Shi¹,

Cheng²,

Zhang³

et al. 2014

IJN

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Magnetic iron oxide nanoparticles (NPs) are emerging as novel materials with great potentials for various biomedical applications, but their biological activities are largely unknown. In the present study, we found that ferroferric oxide nanoparticles (Fe 3 O 4 NPs) induced autophagy in blood cells. Both naked and modified Fe 3 O 4 NPs induced LC3 lipidation and degraded p62, a monitor of autophagy flux. And this change could be abolished by autophagy inhibitors. Mechanistically, Fe 3 O 4 NP-induced autophagy was accompanied by increased Beclin 1 and VPS34 and decreased Bcl-2, thus promoting the formation of the critical complex in autophagy initiation. Further studies demonstrated that Fe 3 O 4 NPs attenuated cell death induced by anticancer drugs bortezomib and doxorubicin. Therefore, this study suggested that Fe 3 O 4 NPs can induce prosurvival autophagy in blood cells by modulating the Beclin l/Bcl-2/VPS34 complex. This study suggests that caution should be taken when Fe 3 O 4 NPs are used in blood cancer patients.

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Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells

Cited by 16 publications

References 45 publications

Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the Beclin 1/Bcl-2/VPS34 complex

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Hemin-coupled iron(III)-hydroxide nanoparticles show increased uptake in Caco-2 cells

Cited by 16 publications

References 45 publications

Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Effect of protein corona magnetite nanoparticles derived from bread in vitro digestion on Caco-2 cells morphology and uptake

Utility of models of the gastrointestinal tract for assessment of the digestion and absorption of engineered nanomaterials released from food matrices

Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the&nbsp;Beclin 1/Bcl-2/VPS34 complex

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Ferroferric oxide nanoparticles induce prosurvival autophagy in human blood cells by modulating the Beclin 1/Bcl-2/VPS34 complex