Hemin-graphene oxide-gold nanoflower-assisted enhanced electrochemiluminescence immunosensor for determination of prostate-specific antigen

Liu, Gengjun; Guan, Xiaohan; Li, Binxiao; Zhou, Hong; Kong, Na; Wang, Haiyan

doi:10.1007/s00604-022-05387-2

Cited by 6 publications

(4 citation statements)

References 36 publications

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“…Hemin is an iron porphyrin derivative with two carboxyl functional groups, and also an efficient catalyst for the luminol-H 2 O 2 system, 21,27,28 which makes it a suitable candidate to construct functionalized MOFs as an organic bridging ligand. In fact, Hemin ligands have already been reported for the synthesis of MOFs.…”

Section: Introductionmentioning

confidence: 99%

Functionalized metal–organic frameworks based on multi-catalyst ordered assembly for electrochemical stripping chemiluminescent immunoassay

Liang,

Hu,

Zheng

et al. 2023

Sens. Diagn.

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Section: Introductionmentioning

confidence: 99%

Functionalized metal–organic frameworks based on multi-catalyst ordered assembly for electrochemical stripping chemiluminescent immunoassay

Liang,

Hu,

Zheng

et al. 2023

Sens. Diagn.

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“…HGNs inherit the strong peroxidase activity of hemin and are less prone to environmental variations [ 23 , 24 ]. In previous studies, graphene-based biosensors have been demonstrated to have many merits, including the composition of multiple functions, choices of detection mechanism, signal generation, and amplification, as well as easy-engineering [ 25 , 26 , 27 , 28 ]. Particularly, biosensors using aptamers selected through systematic evolution of ligands by exponential enrichment (SELEX) have been developed specifically against targets with sizes varying from metal ions to cells [ 29 , 30 , 31 ].…”

Section: Introductionmentioning

confidence: 99%

A Hemin–Graphene Nanocomposite-Based Aptasensor for Ultrasensitive Colorimetric Quantification of Leukaemia Cells Using Magnetic Enrichment

Zhang

Lai

et al. 2022

Biosensors

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Diagnostic blood cell counting is of limited use in monitoring a minimal number of leukaemia cells, warranting further research to develop more sensitive and reliable techniques to identify leukaemia cells in circulation. In this work, a hemin–graphene nanocomposite-based aptasensor was developed for ultrasensitive colorimetric detection of leukaemia cells (CEM) using magnetic enrichment. Hemin-conjugated graphene oxide nanocomposites (HGNs) were prepared by hydrazine reduction using graphene oxide nanosheets and hemins. Hence, the prepared HGNs become able to absorb single-stranded DNA and acquire peroxidase-like activity. The aptamer sgc8c, which recognizes a specific target on leukaemia cells, was absorbed onto HGNs to capture the target CEM cancer cells. The captured target cells that associated with the HGNs were then concentrated and separated by magnetic beads (MBs) coated with sgc8c aptamers, forming a HGN–cell–MB sandwich structure. These sandwich structures can be quantified via an oxidation reaction catalysed by HGNs. By utilizing dual signal amplification effects generated by magnetic enrichment and the improved peroxidase activity of HGNs, the biosensor allowed for highly sensitive detection of 10 to 105 CEM cells with an ultra-low limit of detection (LOD) of 10 cells under optimal conditions. It is expected that the proposed aptasensor can be further employed in monitoring the minimal residual disease during the treatment of leukaemia.

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“…25,26 In addition, functionalization of graphene sheets with hemin was employed for the electro(chemiluminescence)-sensing of different biomolecules. 27,28 Of interest, hemin-functionalized graphene through π−π stacking interactions produced a sensitive sensor for specific electrochemical detection of • NO in physiological environments. 29 Moreover, hemin was utilized as a main component in reaction systems for CL detection of certain biomolecules.…”

mentioning

confidence: 99%

“…For instance, coordination polymer-hemin-based nanocomposites were employed for the detection of different biological analytes via amperometry and luminescence . Moreover, multiple G-quadruplex-hemin DNAzyme and RNAzyme systems with peroxidase-mimicking activity were developed for multidisciplinary applications. , In addition, functionalization of graphene sheets with hemin was employed for the electro(chemiluminescence)-sensing of different biomolecules. , Of interest, hemin-functionalized graphene through π–π stacking interactions produced a sensitive sensor for specific electrochemical detection of ● NO in physiological environments . Moreover, hemin was utilized as a main component in reaction systems for CL detection of certain biomolecules. − …”

mentioning

confidence: 99%

Hemin as a Molecular Probe for Nitric Oxide Detection in Physiological Solutions: Experimental and Theoretical Assessment

Alsharabasy,

Farràs,

Pandit

2024

Anal. Chem.

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Given its pivotal role in modulating various pathological processes, precise measurement of nitric oxide ( • NO) levels in physiological solutions is imperative. The key techniques include the ozone-based chemiluminescence (CL) reactions, amperometric • NO sensing, and Griess assay, each with its advantages and drawbacks. In this study, a hemin/H 2 O 2 /luminol CL reaction was employed for accurately detecting • NO in diverse solutions. We investigated how the luminescence kinetics was influenced by • NO from two donors, nitrite and peroxynitrite, while also assessing the impact of culture medium components and reactive species quenchers. Furthermore, we experimentally and theoretically explored the mechanism of hemin oxidation responsible for the initiation of light generation. Although both hemin and • NO enhanced the H 2 O 2 /luminol-based luminescence reactions with distinct kinetics, hemin's interference with • NO/peroxynitrite − modulated their individual effects. Leveraging the propagated signal due to hemin, the • NO levels in solution were estimated, observing parallel changes to those detected via amperometric detection in response to varying concentrations of the • NO-donor. The examined reactions aid in comprehending the mechanism of • NO/hemin/H 2 O 2 /luminol interactions and how these can be used for detecting • NO in solution with minimal sample size demands. Moreover, the selectivity across different solutions can be improved by incorporating certain quenchers for reactive species into the reaction.

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Hemin-graphene oxide-gold nanoflower-assisted enhanced electrochemiluminescence immunosensor for determination of prostate-specific antigen

Cited by 6 publications

References 36 publications

Functionalized metal–organic frameworks based on multi-catalyst ordered assembly for electrochemical stripping chemiluminescent immunoassay

Functionalized metal–organic frameworks based on multi-catalyst ordered assembly for electrochemical stripping chemiluminescent immunoassay

A Hemin–Graphene Nanocomposite-Based Aptasensor for Ultrasensitive Colorimetric Quantification of Leukaemia Cells Using Magnetic Enrichment

Hemin as a Molecular Probe for Nitric Oxide Detection in Physiological Solutions: Experimental and Theoretical Assessment

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