Hemin with Peroxidase Activity Can Inhibit the Oxidative Damage Induced by Ultraviolet A

Abstract

Excessive reactive oxygen species (ROS), a highly reactive substance that contains oxygen, induced by ultraviolet A (UVA) cause oxidative damage to skin. We confirmed that hemin can catalyze the reaction of tyrosine (Tyr) and hydrogen peroxide (H2O2). Catalysis was found to effectively reduce or eliminate oxidative damage to cells induced by H2O2 or UVA. The scavenging effects of hemin for other free-radical ROS were also evaluated through pyrogallol autoxidation, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH·)-scavenging assays, and phenanthroline–Fe2+ assays. The results show that a mixture of hemin and tyrosine exhibits strong scavenging activities for H2O2, superoxide anion (O2−·), DPPH·, and the hydroxyl radical (·OH). Furthermore, the inhibition of oxidative damage to human skin keratinocyte (HaCaT) cells induced by H2O2 or UVA was evaluated. The results show that catalysis can significantly reduce the ratio of cell apoptosis and death and inhibit the release of lactate dehydrogenase (LDH), as well as accumulation of malondialdehyde (MDA). Furthermore, the resistance to apoptosis was found to be enhanced. These results show that the mixture of hemin and tyrosine has a significantly protective effect against oxidative damage to HaCaT cells caused by UVA, suggesting it as a protective agent for combating UVA damage.

FeTOMPP with Peroxidase-Like Activity as Peroxidase Mimics for Colorimetric Sensing of H2O2 and Ethanol

“…It has been observed that hemerelated compounds, such as haptoglobin, hemin, and hematin, exhibit antioxidative effects against hydrogen peroxide, which is a major oxidant [23]. In a study conducted by Hui et al (2022), it was reported that hemin, a porphyrin ring compound containing iron, displayed peroxidase activity [24]. These findings highlight the potential of porphyrins as peroxidase mimetics due to their structural similarity to natural porphyrins.…”

“…The reaction is incubated at 30 °C for 10 min. The concentration of the oxidized TMB was quantified by UV-Vis absorption spectroscopy at 652 nm by using a molar extinction coefficient of  = 3.9×104 M −1 cm −1 [25].…”

“…It has been reported that heme-related compounds, such as haptoglobin, hemin and hematin exert anti-oxidative effect that hydrogen peroxide is one of the major oxidants [23]. Hui et al (2022) have been reported that hemin, a porphyrin ring compound containing iron, had the peroxidase activity [24]. Therefore, porphyrins are interesting compounds to be tested as peroxidase mimetics because of their structural analogy with those of natural porphyrins.…”

“…In previous studies, researchers have explored the functionalization of porphyrins with materials like NiO, Fe3O4, and Co3O4 [25][26][27]. These functionalized porphyrins have exhibited enhanced peroxidase activity and have been capable of catalyzing reactions between dye substrates and H2O2, leading to color reactions that can be visually observed.…”