Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)

Tkachenko, Anton

doi:10.1016/j.tiv.2024.105814

Cited by 3 publications

(3 citation statements)

References 287 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have selected L929 cells, which are broblast-like cells considered one of the most abundant cell types in the connective tissue, and mature erythrocytes, which are the most abundant cells in the circulation. Notably, due to the lack of organelles erythrocytes have been reported to be a convenient model in nanotoxicology to examine the mechanisms of cytotoxicity of nanoscale materials (Tkachenko 2024b). 2023).…”

Section: Discussionmentioning

confidence: 99%

“…of hemocompatibility is an important step in the overall assessment of safety and biocompatibility of nanomaterials (de la Harpe et al 2019),(Yedgar et al 2022),(Tkachenko 2024b). In the current study, hemolytic and osmotic fragility assays have revealed that ZnCH are characterized by good hemocompatibility and low toxicity against erythrocytes.…”

mentioning

confidence: 99%

“…Additionally, eryptosis assays have been used to supplement data of hemolysis assay and osmotic fragility test. Notably, eryptosis has been shown to be more sensitive in nanotoxicological studies than hemolysis(Lau et al 2012),(Ran et al 2015),(Xu et al 2016),(Ferdous et al 2018),(Tkachenko 2024b). Indeed, our ndings are consistent with this conclusion and indicate that eryptosis can be induced by much lower concentrations of ZnCH micro akes than hemolysis.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Prokopiuk,

Onishchenko,

Pazura

et al. 2024

Preprint

View full text Add to dashboard Cite

Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity of the microflakes was assessed against murine L929 cell line and rat mature erythrocytes. MTT and neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, Fura 2-AM staining, staining for reactive nitrogen species (RNS), detection of caspases, and lipid order-sensitive NR12S probe staining were employed to evaluate cell viability, motility, cell death pathways, implication of Ca2+, reactive oxygen species (ROS) and RNS signaling, caspases, and alterations of cell membranes following exposure to the microflakes, respectively. To assess hemocompatibility of the Zn-containing microflakes, osmotic fragility assay and hemolysis assay were performed, as well as multiple eryptosis parameters were evaluated. Results. Our findings indicate a dose-response cytotoxicity of ZnCH microflakes against L929 cells with no toxicity observed for low concentrations (10 mg/L and below). At high concentrations, ZnCH microflakes promoted oxidative stress, Ca2+- and caspase-dependent apoptosis and altered lipid membranes. Surprisingly, toxicity to enucleated mature erythrocytes was found to be lower compared to L929 cells. ZnCH microflakes induced eryptosis associated with oxidative stress, Ca2+ signaling and recruitment of caspases. Eryptosis assays were found to be more sensitive than evaluation of hemolysis. Conclusions. Zn5(CO3)(OH)6 microflakes show no cytotoxicity at low concentrations indicating their potential as a source of zinc for livestock animals.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Prokopiuk,

Onishchenko,

Pazura

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

Exploring the effect of hydroxyapatite nanoparticle shape on red blood cells and blood coagulation

Sun,

Li,

Zhong

et al. 2024

Nanomedicine

View full text Add to dashboard Cite

Nanostructured zinc carbonate hydroxide microflakes: assessing the toxicity against erythrocytes and L929 cells in vitro

Prokopiuk,

Onishchenko,

Pazura

et al. 2024

Nanotechnology

View full text Add to dashboard Cite

Introduction. Nanostructured materials have been suggested to be used as a source of dietary zinc for livestock animals. In this study, we assessed the cytotoxicity of newly synthesized nanostructured zinc carbonate hydroxide (ZnCH) Zn5(CO3)(OH)6 microflakes. Materials and methods. Cytotoxicity of the microflakes was assessed against murine L929 cell line and rat mature erythrocytes. MTT and neutral red uptake assays, scratch assay, Annexin V-FITC/7-aminoactinomycin D staining, 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining, Fura 2-AM staining, staining for reactive nitrogen species (RNS), detection of caspases, and lipid order-sensitive NR12S probe staining were employed to evaluate cell viabilityViability, motility, cell death pathways, implication of Ca2+, reactive oxygen species (ROS) and reactive nitrogen species (RNS) signaling, caspases, and alterations of cell membranes following exposure of L929 cells to the microflakes, respectively were assessed. To assess hemocompatibility of the Zn-containing microflakes, osmotic fragility assay and hemolysis assays were performed, as well as multiple eryptosis parameters were evaluated.Results. Our findings indicate a dose-response cytotoxicity of ZnCH microflakes against L929 cells with no toxicity observed for low concentrations (10 mg/L and below). At high concentrations (25 mg/L and above), ZnCH microflakes promoted oxidative stress,nitrosyl stress, Ca2+- and caspase-dependent apoptosis, and altered lipid order of cell membranes in a dose-dependent manner, evidenced by up to 7-fold elevation of RNS-dependent fluorescence, 2.9-fold enhancement of Fura 2-dependent fluorescence, over 20-fold elevation of caspases-dependent fluorescence (caspase-3, caspase-8, and caspase-9), and up to 4.4-fold increase in the ratiometric index of the NR12S probe. Surprisingly, toxicity to enucleated mature erythrocytes was found to be lower compared to L929 cells. ZnCH microflakes induced eryptosis associated with oxidative stress, nitrosyl stress, Ca2+ signaling and recruitment of caspases at 25-50-100 mg/L. Eryptosis assays were found to be more sensitive than evaluation of hemolysis. Conclusions. Zn5(CO3)(OH)6 microflakes show no cytotoxicity at low concentrations indicating their potential as a source of zinc for livestock animals.

show abstract

Hemocompatibility studies in nanotoxicology: Hemolysis or eryptosis? (A review)

Cited by 3 publications

References 287 publications

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Nanostructured Zinc Carbonate Hydroxide Microflakes: Assessing the Toxicity Against Erythrocytes and L929 Cells in Vitro

Exploring the effect of hydroxyapatite nanoparticle shape on red blood cells and blood coagulation

Nanostructured zinc carbonate hydroxide microflakes: assessing the toxicity against erythrocytes and L929 cells in vitro

Contact Info

Product

Resources

About