Hemocompatibility tuning of an innovative glutaraldehyde-free preparation strategy using riboflavin/UV crosslinking and electron irradiation of bovine pericardium for cardiac substitutes

Dittfeld, Claudia; Welzel, Cindy; König, Ulla; Jannasch, Anett; Alexiou, Konstantin; Blum, Ekaterina; Bronder, Saskia; Sperling, Claudia; Maitz, Manfred F.; Tugtekin, Sems Malte

doi:10.1016/j.bioadv.2023.213328

Cited by 2 publications

(3 citation statements)

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“…35 As the SULEEI procedure is a novel GA-free strategy to produce xenogenic pericardial material for biological aortic valve (AV) prostheses and patch material, 28 this is the first report about the cellular response after implantation of SULEEI-pericardium into a living vertebrate. As recently shown, the SULEEI protocol incorporates efficient decellularization, resulting in the elimination of α-Gal epitopes and DNA fragments to less than 300 bp, 30 which is reported to be nonimmunogenic. 36 Our results demonstrate that SULEEI-pericardium evokes a similar immune reaction and tissue response as the clinically used control standard bovine patch material.…”

Section: A Cellular and Tissue Responsementioning

confidence: 99%

“…28 This protocol, developed for porcine tissue, was adapted for thicker bovine tissue. [29][30][31] Pericardial tissue was stored in 5 mM Tris(hydroxymethyl)aminomethane (Tris, pH = 8) for 60 h, containing 2% phenoxyethanol to inhibit bacterial growth. Within the first sterilization step, tissue was incubated in 1% Triton X-100 in 5 mM Tris (pH = 8) at 4 ○ C for 8 h, followed by DNAse (0.1 mg/ml), RNAse (0.02 mg/ml), and trypsin (0.004 μg/ml) in HBSS at 37 ○ C for 15 h. Subsequently, again, an incubation in 1% Triton X-100 in 5 mM Tris (pH = 8) at 4 ○ C for 7 h took place.…”

Section: Experimental a Preparation Of Pericardial Implantsmentioning

confidence: 99%

“…28 The resulting pericardial tissue exhibits improved cytotoxicity, comparable hemocompatibility, and mechanical properties to GA-treated tissue and shows a retained α-helical structure, which is supposed to be less prone to deterioration. [28][29][30][31] The herein presented comprehensive testing of SULEEI-pericardium for biocompatibility and calcification potential after subcutaneous implantation in vivo is an essential requirement for ongoing large animal testing in aortic valve position and further clinical application.…”

Section: Introductionmentioning

confidence: 99%

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In vivo application of a glutaraldehyde-free, UVA/riboflavin cross-linked bovine pericardium confirms suitability for cardiovascular substitutes

Jannasch,

Welzel,

Pablik

et al. 2024

APL Materials

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Glutaraldehyde (GA)-treated bovine pericardium is still the gold standard for the fabrication of bioprostheses needed for the surgical treatment of valvular malfunction. Although excellent stability and low immunogenicity are accomplished, the application of GA is considered to be causal for structural valve deterioration, diminishing the long-term durability of bioprosthetic tissue. The novel GA-free SULEEI-treatment of bovine pericardium combines decellularization, riboflavin/UVA-cross-linking, and low-energy electron beam irradiation. In the present study, we initiated an in vivo application. We used a subcutaneous rat model to compare the immune and tissue responses, calcification propensity, and biomechanical properties of the alternatively prepared SULEEI bovine pericardial tissue with standard glutaraldehyde-fixed and industrially produced bovine pericardial patch material. SULEEI pericardium evokes a similar immune reaction and tissue response as the control standard bovine patch material. The calcification propensity of SULEEI tissue was low, and biomechanical analysis revealed a heterogeneous but similar pattern in tissue stiffness compared to the control patch. The results of this study highlight the potential of SULEEI-treated bovine pericardial tissue as a candidate for cutting-edge cardiovascular and valvular biomaterials in reconstructive surgery.

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Section: A Cellular and Tissue Responsementioning

confidence: 99%

Section: Experimental a Preparation Of Pericardial Implantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vivo application of a glutaraldehyde-free, UVA/riboflavin cross-linked bovine pericardium confirms suitability for cardiovascular substitutes

Jannasch,

Welzel,

Pablik

et al. 2024

APL Materials

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Deciphering UVA/Riboflavin Collagen Crosslinking: A Pathway to Improve Biomedical Materials

Fan,

Jung,

Herrmann

et al. 2024

Adv Funct Materials

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Collagen crosslinking employing ultraviolet A rays and riboflavin (UVA/R) has emerged as a pivotal technique in clinical therapies, especially in ophthalmology since the 1990s. Despite its clinical adoption, the lack of clarity of the detailed mechanism and the imperative for a refined manufacturing process necessitates further investigation. This study advances the understanding of UVA/R crosslinked collagen, concentrating on identifying the primary crosslinking sites using seven synthetic peptides and exploring the pathways of riboflavin‐mediated crosslinking. The results demonstrate that tyrosine residues are key crosslinking sites, and riboflavin plays a dual role as both a catalyst and a competitive inhibitor in the crosslinking process. Furthermore, the UVA/R crosslinked collagen matrix exhibits a more harmonious balance between stability and degradability compared with chemically crosslinked collagen matrices, coupled with superior mechanical properties and augmented biocompatibility. In vivo experiments further validate its excellent biocompatibility, reduced tissue inflammation, and promotion of tissue regeneration. The research provides crucial insights into collagen crosslinking mechanisms, paving the way for the development of sophisticated collagen‐based biomaterials tailored for biomedical applications.

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Hemocompatibility tuning of an innovative glutaraldehyde-free preparation strategy using riboflavin/UV crosslinking and electron irradiation of bovine pericardium for cardiac substitutes

Cited by 2 publications

References 75 publications

In vivo application of a glutaraldehyde-free, UVA/riboflavin cross-linked bovine pericardium confirms suitability for cardiovascular substitutes

In vivo application of a glutaraldehyde-free, UVA/riboflavin cross-linked bovine pericardium confirms suitability for cardiovascular substitutes

Deciphering UVA/Riboflavin Collagen Crosslinking: A Pathway to Improve Biomedical Materials

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