Hemocyanin levels in Octopus vulgaris and the cuttlefish Sepia officinalis from the aegean sea

Senozan, N. M.; Avinc, A.; Unver, Zehra

doi:10.1016/0300-9629(88)90638-x

Cited by 17 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, Aguila et al (2007) obtained values of blood hemocyanin in Octopus maya between 1.65 and 2 mmol/L, with no differences between specimens fed crab and those fed most formulated diets with CSP. Senozan et al (1988) obtained a hemocyanin concentration from 58.4 to 88.9 g/L in Sepia officinalis and 47.6-66.9 g/L in Octopus vulgaris from the copper content. According to Ellerton et al (1983) the respiratory molecule contains 0.25% by weight copper.…”

Section: Discussionmentioning

confidence: 99%

Is Copper Supplementation Required in Formulated Feeds forOctopus vulgaris(Cuvier, 1797)?

Sancho¹,

Valverde²,

Sironi³

et al. 2015

Journal of Shellfish Research

View full text Add to dashboard Cite

The aim of this study was to test the effect of copper supplementation in a formulated feed on the growth, feed efficiency, and nutritional composition of subadults Octopus vulgaris, with particular reference to the differences in the content of copper in the tissues (muscle, digestive gland, and hemolymph). Two formulated feeds were supplied to subadults octopuses kept individually for 56 days: Basal diet [11.6 mgCu/kg dry weight (dw)], and another feed with a similar composition but including a copper supplementation (copper diet: 115.2 mgCu/kg dw). No significant differences (P > 0.05) were detected in the respective growth rates (0.88% body weight/day), FE (79.5%-82.1%), proximate composition, hemolymph hemocyanin (1.6-1.8 mmol/L), hemolymph copper (0.19-0.20 mgCu/mL), or muscle copper levels (22.7-23.2 mgCu/kg dw). No significant changes were detected on proximate composition, hemocyanin and hemolymph, and muscle copper levels between animals fed formulated feeds and an initial group fed natural diet based on crab and fish (P > 0.05). The only parameter which showed a significant difference (P < 0.05) was the copper level in the digestive gland: 1,797, 390, and 1,148 mgCu/kg dw for initial (natural diet), basal and copper diet, respectively (P < 0.05). Considering the experimental conditions of this study, copper supplementation is not effective in improving the performance of a formulated feed for octopus.

show abstract

Section: Discussionmentioning

confidence: 99%

Is Copper Supplementation Required in Formulated Feeds forOctopus vulgaris(Cuvier, 1797)?

Sancho¹,

Valverde²,

Sironi³

et al. 2015

Journal of Shellfish Research

View full text Add to dashboard Cite

show abstract

“…On the one hand, the fluorescence labeling method with 5-(6)-carboxyfluorescein-N-hydroxysuccinimide ester has an advantage over fluorescein isothiocyanate (FITC) in that it allows an effective labeling at pH 7.4 and in that the amide bond formed after the reaction is more stable than the thio-urea bond formed between FITC and free amino groups. As a consequence, unwanted binding of pure non-inactivated fluorescein during the control injection into the circulatory system could not be ruled out, i.e., it is quite likely that autolabeling reactions between fluorescein molecules and mainly hemocyanin take place, because the native hemolymph has a pH of 7.4 and a hemocyanin content of about 80% (D'Aniello et al 1986;Senozan et al 1988;). Finally, there were no experimental differences between the injection of the fluorescein-labeled Sepia hemocyanin and the pure fluorescein, i.e., as for the gold-labeled Sepia hemocyanin, the conjugated fluorescein molecules mask the receptor-binding sites and therefore no fluoresceinlabeled Sepia hemocyanin occurred in the rhogocytes.…”

Section: In Situ Injection Of Fluorescein-labeled Sepia Hemocyaninmentioning

confidence: 98%

Hemocyanin and the branchial heart complex of Sepia officinalis : are the hemocytes involved in hemocyanin metabolism of coleoid cephalopods?

Beuerlein

Ruth

Westermann

et al. 2002

Cell and Tissue Research

View full text Add to dashboard Cite

Cytobiological experiments using isotopic- and cytochemical-labeled Sepia hemocyanin as well as immunocytochemical localization of the respiratory pigment were carried out to investigate the function of the hemocytes in hemocyanin metabolism of the common cuttlefish Sepia officinalis. For comparison, the rhogocytes (ovoid cells) of the branchial heart complex were included in this study. Hemocyanin molecules were immunocytochemically detected in the lysosomal compartment of the rhogocytes and, at lower levels, in adhesive and circulating hemocytes. (125)I-labeled Sepia hemocyanin was taken up by the rhogocytes only, whereas gold- and/or fluorescein-labeled Sepia hemocyanin was solely taken up by the adhesive and the circulating hemocytes, even though the level of uptake is different. There are also differences in the uptake of pure gold particles and/or fluorescein between rhogocytes and hemocytes. These findings give evidence that circulating and adhesive hemocytes of the branchial heart complex are not involved in hemocyanin turnover, but are a component of the cellular defense and detoxification system of adult coleoid cephalopods.

show abstract

“…and Se-pia officinalis L. (D'Aniello et al 1986;Senozan et al 1988). The molecular weights of the two hemocyanin fragments of the unprocessed hemolymph and the purified hemocyanin after dialysis (237.9 kDa and 166.2 kDa) obtained by gel electrophoresis may be attributed to the effect of ionization and linearization when using SDS-polyacrylamide gels.…”

Section: Results From the Biochemical Studiesmentioning

confidence: 97%

Cytobiological studies on hemocyanin metabolism in the branchial heart complex of the common cuttlefish Sepia officinali s (Cephalopoda, Dibranchiata)

Beuerlein

Schimmelpfennig²,

Westermann³

et al. 1998

Cell and Tissue Research

View full text Add to dashboard Cite

The present study confirms previous investigations that demonstrated a high copper content in the branchial heart and its appendage, and that gave the first indication that this organ complex might be involved in hemocyanin metabolism in Sepia officinalis L. Immunocytochemical localization of hemocyanin molecules within the endocytotic lysosomal system of the ovoid cells and tracer experiments with 125I-labeled Sepia hemocyanin suggest its endocytotic uptake. Energy-dispersive X-ray microanalysis and histochemical methods reveal a high copper content within the ovoid cells of the branchial heart. In view of the turnover of the respiratory pigment in the branchial heart of Sepia officinalis L., we believe that the ovoid cells are a site of hemocyanin catabolism.

show abstract

Hemocyanin levels in Octopus vulgaris and the cuttlefish Sepia officinalis from the aegean sea

Cited by 17 publications

References 21 publications

Is Copper Supplementation Required in Formulated Feeds forOctopus vulgaris(Cuvier, 1797)?

Is Copper Supplementation Required in Formulated Feeds forOctopus vulgaris(Cuvier, 1797)?

Hemocyanin and the branchial heart complex of Sepia officinalis : are the hemocytes involved in hemocyanin metabolism of coleoid cephalopods?

Cytobiological studies on hemocyanin metabolism in the branchial heart complex of the common cuttlefish Sepia officinali s (Cephalopoda, Dibranchiata)

Contact Info

Product

Resources

About