Hemocyte Types and Their Primary Cultures in the Argasid Tick, Ornithodoros moubata MURRAY (Ixodoidea)

Fujisaki, Kozo; Kitaoka, Shigeo; Morii, Tsutomu

doi:10.1303/aez.10.30

Cited by 21 publications

(17 citation statements)

References 17 publications

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“…Interestingly, Drosophila SR macrophage/hemocytes are attractive candidates for insect immunity, and its expression and physiological functions have been reported (Pearson et al 1995). However, at least two types of phagocytic cells in ticks, plasmatocytes and granulocytes, have been reported (Dolp 1970;Fujisaki et al 1975;Kuhn and Haug 1994;Zhioua et al 1996;Inoue et al 2001); here, we show the mRNA expression of the HlSRB gene in hemocytes, a finding that would contribute to future study of the functional conservation on innate immunity in H. longicornis using hemocytes.…”

Section: Introductionsupporting

confidence: 60%

“…Tissues were collected from partially fed ticks by dissection. Hemolymphs were collected from the coxal-trochanteral tick joints and drawn into capillary tubes (Fujisaki et al 1975;Inoue et al 2001) containing 100 μl of phosphate-buffered saline (PBS), on ice. Hemocytes were obtained using a centrifuge at 100×g for 5 min at 4°C.…”

Section: Rna Isolation and Reverse Transcriptase-polymerase Chain Reamentioning

confidence: 99%

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Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis

et al. 2010

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Scavenger receptors (SRs) are cell-surface proteins and exhibit distinctive ligand-binding properties, recognizing a wide range of ligands that include microbial surface constituents and intact microbes. The class B scavenger receptor CD36 (SRB) is predominantly expressed by macrophages and is considered important in innate immunity. We here show the identification and characterization of SRB from the hard ixodid tick, Haemaphysalis longicornis (HlSRB). The full-length cDNA was 2,908 bp, including an ORF encoding of 1,518 amino acids with a pI value of 5.83. H. longicornis SRB contains a hydrophobic SRB domain and four centrally clustered cysteine residues for arrangement of disulfide bridges. Deduced amino acid sequence has an identity of 30-38% with the SRB of other organisms. RT-PCR analysis showed that mRNA transcripts were expressed in multiple organs of adult ticks but with a different transcript level in the developmental stages of H. longicornis ticks. His-tagged recombinant HlSRB was expressed in Escherichia coli with an expected molecular mass of 50 kDa. In Western blot analysis, mouse anti-rHlSRB serum recognized a strong reaction with a 50 kDa protein band in lysates prepared from egg and adult tick but showed a weak reaction with lysates of larva and nymph. In an indirect immunofluorescent antibody test, HlSRB antiserum recognized the protein located on the midgut, salivary glands, and ovary of partially fed H. longicornis females. Silencing of the HlSRB gene by RNAi led to a significant reduction in the engorged female body weight. It is noteworthy that more than a dozen SRB orthologs have been identified in the genomes of insect species with functions related to pheromone signaling, innate immunity, phagocytic clearance of apoptotic cells, and various aspects of the fatty acid metabolism. This is the first report of the identification and characterization of the SRB homologue in Chelicerata, including ticks, horseshoe crabs, scorpions, spiders, and mites.

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Section: Introductionsupporting

confidence: 60%

Section: Rna Isolation and Reverse Transcriptase-polymerase Chain Reamentioning

confidence: 99%

Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis

et al. 2010

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“…The Journal of Experimental Biology 216 (10) were collected as described previously (Aung et al, 2011;Fujisaki et al, 1975). RNA extraction was performed based on the manufacturer's protocol.…”

Section: Preparation Of Mouse Anti-ferritin Seramentioning

confidence: 99%

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Galay

Aung

Umemiya‐Shirafuji

et al. 2013

Journal of Experimental Biology

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SUMMARYTicks are obligate hematophagous parasites and important vectors of diseases. The large amount of blood they consume contains great quantities of iron, an essential but also toxic element. The function of ferritin, an iron storage protein, and iron metabolism in ticks need to be further elucidated. Here, we investigated the function a newly identified secreted ferritin from the hard tick Haemaphysalis longicornis (HlFER2), together with the previously identified intracellular ferritin (HlFER1). Recombinant ferritins, expressed in Escherichia coli, were used for anti-serum preparation and were also assayed for iron-binding activity. RT-PCR and western blot analyses of different organs and developmental stages of the tick during blood feeding were performed. The localization of ferritins in different organs was demonstrated through an indirect immunofluorescent antibody test. RNA interference (RNAi) was performed to evaluate the importance of ferritin in blood feeding and reproduction of ticks. The midgut was also examined after RNAi using light and transmission electron microscopy. RT-PCR showed differences in gene expression in some organs and developmental stages. Interestingly, only HlFER2 was detected in the ovary during oviposition and in the egg despite the low mRNA transcript. RNAi induced a reduction in post-blood meal body weight, high mortality and decreased fecundity. The expression of vitellogenin genes was affected by silencing of ferritin. Abnormalities in digestive cells, including disrupted microvilli, and alteration of digestive activity were also observed. Taken altogether, our results show that the iron storage and protective functions of ferritin are crucial to successful blood feeding and reproduction of H. longicornis. Supplementary material available online at

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“…Compared to insects, ticks are only poorly known in respect to their hemocytes characteristics and functions. Few studies have been published on the morphology and classification of tick circulating cells, covering less than a dozen tick species: the genera Ixodes, Dermacentor and Hyalomma representing hard ticks (Dolp 1970;Brinton and Burgdorfer 1971;Eggenberger et al 1990;Al-Khalifa and Siddiqui 1993;Kuhn and Haug 1994;Kuhn 1996;Zhioua et al 1996), and the genera Argas and Ornithodoros representing soft ticks (Dolp 1970;Fujisaki et al 1975;El Shoura 1986, 1989Inoue et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Ontogeny of tick hemocytes: a comparative analysis of Ixodes ricinus and Ornithodoros moubata

Borovičková

Hypša

2005

Exp Appl Acarol

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Hemocytes of two tick species, Ixodes ricinus and Ornithodoros moubata, were investigated with the aim to determine their ultrastructural characteristics and developmental relationships. Only a limited number of ultrastructural features was shown to be unequivocally homological across all hemocyte types. The two species, representing distant groups of ticks, differ in the composition of their circular cell populations. In I. ricinus, three groups of distinct morphological types of hemocytes could be determined according to well-defined ultrastructural features: a typical non-phagocytic granular cell with electron-dense granula and homogeneous cytoplasm (Gr II), and two different types of phagocytic hemocytes, namely plasmatocytes with a low number of granula and phagocytic granolocytes, designated as Gr I. In contrast, an additional cell type resembling insect spherulocytes was determined in O. moubata. This cell type does not seem to be homologous to any I. ricinus hemocyte and may represent a cell type typical of soft ticks only. Possible ontogenetic lineages of the hemocytes of both tick-species were inferred.

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Hemocyte Types and Their Primary Cultures in the Argasid Tick, Ornithodoros moubata MURRAY (Ixodoidea)

Cited by 21 publications

References 17 publications

Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis

Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis

Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis

Ontogeny of tick hemocytes: a comparative analysis of Ixodes ricinus and Ornithodoros moubata

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