Hemodynamic and blood biochemical profile during endotoxemia and after therapeutic intervention in buffalo calves.

Abstract

Five apparently healthy male buffalo calves aged between 6-12 months were infused with Escherichia coli endotoxin @5μg/kg BW/hr for 3 hours to induce the symptoms of endotoxemia like corneal opacity, increased respiration rate, hypoproteinemia, hypoalbuminemia, hypoglycemia, decrease in mean systolic, diastolic, pulse and mean arterial and central venous pressure along with inconsistent changes in heart rate in the present investigation. The endotoxemic animals were then treated with hypertonic saline solution (HSS) @ 4 ml/Kg BW, Dextran-40 @ 10 ml/Kg BW, Flunixin Meglumine 1.1 mg/Kg BW and blood @ 20 ml/Kg BW as a single infusion which raised the systolic, diastolic, pulse, mean arterial and central venous pressure along with hematocrit and hemoglobin to the levels either close or even higher than the normal pre-infusion levels. Respiration rate and body temperature showed non-significant alterations during endotoxin infusion and after therapeutic intervention. A non-significant increase in total protein was observed after treatment which reached close to pre-infusion level. Mean plasma fibrinogen showed non-significant rise throughout the period of observation while plasma albumin, globulin and creatinine varied non-significantly. A significant increase was seen in plasma glucose level at 5th, 6th and 7th hour as compared to pre-infusion normal values. Overall, treatment given to the animals was found to be effective.