Hemodynamic response of ovarian artery after hCG injection

Waseda, T.; Makinoda, S.; Watanabe, Yukio; Sasakura, Chihaya; Imafuku, Noriaki; Hirosaki, N; Inoue, Haruhiro; Ohshima, K; Fujii, Ryota; Iura, T.

doi:10.1016/s0303-7207(03)00065-0

Cited by 7 publications

(5 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was reported that FBF, measured with colour flow Doppler, increased 1 h after administering hCG. It has been found that ovarian blood flow increases 1–2 h after administration of hCG in humans (Waseda et al. 2003).…”

Section: Discussionmentioning

confidence: 99%

“…It was reported that FBF, measured with colour flow Doppler, increased 1 h after administering hCG. It has been found that ovarian blood flow increases 1-2 h after administration of hCG in humans (Waseda et al 2003). Furthermore, blood flow in the ovaries rapidly increased up to 30-75% in rabbits following hCG administration (Janson 1975;Makinoda 1980).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of Induction of Ovulation with GnRH or hCG on Follicular and Luteal Blood Flow in Holstein–Friesian Heifers

Aslan

Arslanbas

Beindorff

et al. 2011

Reprod Domestic Animals

View full text Add to dashboard Cite

The objective of this study was to compare the effects of gonadotropin-releasing hormone (GnRH) and human chorionic gonadotropin (hCG) on follicular blood flow (FBF) during the pre-ovulatory period and on luteal blood flow (LBF) during dioestrus in Holstein-Friesian heifers. Twelve animals were randomly divided into two groups and treated with either intramuscular injection of 100 μg GnRH or intravenous (IV) injection of 5000 IU hCG on Day 0 (oestrus, 48 h after administration of PGF(2α) ) to induce ovulation. Follicular size (FS), FBF and time of ovulation were recorded with colour Doppler sonography at 0, 1, 3, 6, 12 and 24 h after GnRH and hCG treatment. Luteal size (LS) and LBF were investigated on Day 9 and 12 after ovulation. Plasma samples were taken to determine total oestrogens (E(tot) ) and progesterone (P(4) ) after each examination. Ovulation occurred between 24 and 48 h after treatment in all animals. No difference (p > 0.05) was observed in FS between the two treatment groups. Follicular blood flow was higher in the hCG group than that was in GnRH group at 1 h after treatment (p < 0.01). Total oestrogens were also higher (p < 0.01) in the hCG group than GnRH group; however, this difference was only obvious at 12 h after treatment. No difference (p > 0.05) was observed in LS, LBF or P(4) levels on Day 9 and 12 between treatment groups. In conclusion, the results suggest that induction of ovulation with hCG and GnRH has a temporary effect on FBF and oestrogen levels while no effect on the size of corpora lutea, LBF and P(4) levels was observed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of Induction of Ovulation with GnRH or hCG on Follicular and Luteal Blood Flow in Holstein–Friesian Heifers

Aslan

Arslanbas

Beindorff

et al. 2011

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…The data recorded in this study do reveal an increase in P4 concentration but not in luteal morphology, as observed by Gamboni et al (1984) and Vergani et al (2020). Likewise, the treatment did not affect ovarian perfusion, which is contrary to expectations due to the angiogenic effects of hCG (Waseda et al, 2003). Both groups displayed the same luteal characteristics (morphology, perfusion, and echogenicity) even during structural luteolysis.…”

Section: Discussioncontrasting

confidence: 79%

Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

Dias¹

View full text Add to dashboard Cite

Multiple ovulation and embryo transfer (MOET) play a key role in accelerating sheep farming, but it is still limited by obstacles such as embryo recovery technique and variability in the superovulatory (SOV) response. This study evaluates alternatives to these limitations, aiming to increase in vivo embryo production in sheep. The first chapter reviews some relevant points of non-surgical embryo recovery (NSER): ovine cervical anatomy, physiological mechanisms of cervical relaxation, and current cervical relaxation protocols (PRC). The second and third chapters evaluate the efficiency of PRC using zero (0.0mg), half (0.5mg), or full dose (1.0mg) of estradiol benzoate (EB) in synchronized and/or superovulated ewes and its effects on NSER feasibility and luteal function. Sheep were randomly assigned to receive 37.5 µg of d-cloprostenol i.m. and 0.0 mg (0EB group; n=12); 0.5 mg (0.5EB group; n=12) or 1.0 mg EB (1.0EB group, n=12) 16 h before and 50 IU oxytocin i.v. 20 minutes before the NSER. In synchronized sheep, NSER was performed in 91.7% (33/36) with a longer transposition duration with Hegar dilator and longer catheter-mandrel (P<0.05) in 0.0EB. The treatments did not differ (P>0.05) in the duration of uterine flushing or the rate of structure recovery. In superovulated ewes, there was no treatment or category effect (P>0.05) concerning NSER success. There was a reduction (P<0.05) in ovarian biometry and progesterone (P4) concentration and an increase in estradiol (E2) concentration on D17. The fourth chapter assesses the effectiveness of hCG administration after ovulation. Ovarian function, P4 profile, and embryo yield were evaluated. Superovulated ewes received 300 IU of hCG i.m. (GhCG, n=24) or not (GControl, n=25) 96 h after P4 removal. NSER efficiency, SOV response, and luteal tissue area were similar between groups (P>0.05). The hCG increased (P<0.05) P4 levels and tended to prevent structural luteolysis (P=0.07) but had no effect (P>0.05) on functional luteolysis. The fifth chapter studies the effect of 250, 333, or 400 IU of a commercial pFSH (1FSH: 1LH) on follicular growth, SOV response, and embryo yield. Superovulated ewes with decreasing doses of 250 (G250), 333 (G333), or 400 IU (G400) of pFSH were submitted to NSER. Estrus response, estrus interval, CL and LUF counts and follicular growth did not differ (P>0.05) between groups. The treatments did not differ (P>0.05) in embryo yield, but G333 and G400 showed a tendency (P=0.06) to have a higher mean of recovered structures compared to G250. This study concludes that: 1) NSER can be successfully performed in different sheep breeds, regardless of category, using a cervical relaxation protocol without estradiol benzoate; 2) the use or not of EB affected the luteal function, interfering mainly in estradiol levels; 3) hCG treatment increased circulating P4 before NSER and may prevent early luteal regression; 4) protocols using 250, 333 and 400 IU of pFSH are efficient in promoting follicular growth and superovulatory responses, but 333 IU appears to be more efficient in increasing embryo yield. Keywords: Biotechnologies. Embryos. MOET. Transcervical.

show abstract

“…The V max increased and flow index reduced in the ovarian artery from 15 minutes after hCG injection in the case of ovulation, while nonovulatory cycle demonstrated no significant change. 43 Intraovarian blood flow impedance should be low before the periovulatory phase of a natural cycle to achieve pregnancy, because PI values determined by transvaginal Doppler flow study severely decreased in pregnant cycles. 44 In addition, ovarian follicular vascularity was studied and many significant results were obtained.…”

Section: Monitoring Folliclesmentioning

confidence: 99%

Ultrasonic Assessment of Infertility

Honda¹

2007

Donald School Journal of Ultrasound in Obstetrics and Gynecology

View full text Add to dashboard Cite

Ultrasonographic changes of endometrium, ovaries and salpinges detected by realtime B-mode and 3D ultrasound indicate effective treatment of infertility not only in the Assisted Reproduction Technology (ART) but also in the conventional management where essential plan for the therapy is indicated. The articles concerning ultrasonographic assessments of fecundity and infertility are reviewed. Although the ART has become ordinary treatment of infertility, the pregnancy rate is yet unsatisfactory and it is important to select cycles where favorable conception is predicted in order to increase pregnancy rate in ART. Although antral follicle count (AFC) is the index for predicting ovarian response to human menopausal gonadotropin (hMG) stimulation, it is able to select stimulation options for a poor responder of low AFC value. Since three-dimensional (3D) ultrasonography with power Doppler angiography (PDA) indicates an organ blood flow as a whole, they are important tools for the evaluation of perfusion. The assessment of perifollicular blood flow in preovulatory follicles may be useful for predicting conception and cancelling in vitro fertilization (IVF) in poor vascularity cycle. Since rich endometrial vascularity assessed by 2D and 3D flow signs and the low impedance to flow suggests well perfused endometrium and the high receptivity in the embryo transfer (ET), and vice versa, it would be recommended to limit the number of embryos for the ET in the high receptivity, while to cryopreserve the embryo in case of bad flow signs and wait the cycle of favorable perfusion in order to successfully transfer the embryo.

show abstract

Hemodynamic response of ovarian artery after hCG injection

Cited by 7 publications

References 11 publications

Effects of Induction of Ovulation with GnRH or hCG on Follicular and Luteal Blood Flow in Holstein–Friesian Heifers

Effects of Induction of Ovulation with GnRH or hCG on Follicular and Luteal Blood Flow in Holstein–Friesian Heifers

Strategies to improve cervical relaxation, ovarian response, and embryo yield in sheep undergoing non-surgical embryo recovery

Ultrasonic Assessment of Infertility

Contact Info

Product

Resources

About