Hemoglobin Recognition by Imprinting in Semi-Interpenetrating Polymer Network Hydrogel Based on Polyacrylamide and Chitosan

Xia, Yongqing; Guo, Tianying; Song, Mou-Dao; Zhang, Banghua; Zhang, Baolong

doi:10.1021/bm050324l

Cited by 117 publications

(84 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Because of the properties of chitosan and polyacrylamide, several works have explored the feasibility of a polyacrylamide/chitosan blend. These studies have reported different aspects of the characterization, biocompatibility, and release properties of polyacrylamide/chitosan hydrogels [25][26][27]. Synthesis method, swelling kinetics, and Young's moduli for a polyacrylamide/chitosan hybrid polymeric network at various chitosan concentrations, and different swelling media are reported in this work.…”

Section: Introductionmentioning

confidence: 94%

Swelling characterization and drug delivery kinetics of polyacrylamide-co-itaconic acid/chitosan hydrogels

Martínez-Ruvalcaba¹,

Sánchez-Díaz²,

Becerra³

et al. 2009

Express Polym. Lett.

116

View full text Add to dashboard Cite

Abstract. Hybrid polymeric networks composed of polyacrylamide and chitosan were developed to determine their swelling and ascorbic acid delivery kinetics at various chitosan concentrations. The hybrid acrylamide/chitosan hydrogels were synthesized in aqueous itaconic acid solution (1% w/w). Young's modulus was also evaluated for the hydrogels, and the results were correlated with the swelling properties. Swelling experiments were carried out using three different pH solutions: acidic (pH 4 buffer solution), neutral (distilled water) and basic (pH 10 buffer solution). The results of the swelling study showed that the swelling properties of the network varied with the changes of the pH in the swelling solution, as well as concentration of chitosan. When chitosan concentration increased, the swelling capacity diminished, and therefore Young's modulus increased. The results indicated that the swelling process followed a second order kinetics. The ascorbic acid diffusion inside the hydrogel follows a Fickian mechanism. The ascorbic acid diffusion coefficients are reported as a function of chitosan concentration.

show abstract

Section: Introductionmentioning

confidence: 94%

Swelling characterization and drug delivery kinetics of polyacrylamide-co-itaconic acid/chitosan hydrogels

Martínez-Ruvalcaba¹,

Sánchez-Díaz²,

Becerra³

et al. 2009

Express Polym. Lett.

116

View full text Add to dashboard Cite

show abstract

“…A frequently used approach to evaluate the selectivity is to calculate the ratio of partition coefficients of the template to that of a template analog. The selectivity is usually calculated from re-binding experiments involving either the template or the analog; data from competition assays is very rare [16,17]. Another quality criterion is the imprinting factor (IF), defined as the ratio of maximum binding capacity values of the MIH to the NIH.…”

Section: Characterization Of Mihmentioning

confidence: 99%

“…Recently, Chen et al [12] imprinted lysozyme within stimuli-responsive network and has shown that the molecular recognition process can trigger changes in molecular interactions that can translate into macroscopic response. However, MIHs imprinted with lysozyme as well as with other proteins often display very low binding capacity of no more than 20 mg protein per gram polymer [12,17,18]. Furthermore, the experimental data presented in the previous publications demonstrate that formulations displaying higher binding capacities tend to be less selective.…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted hydrogel displaying reduced non‐specific binding and improved protein recognition

Tov

Luvitch

Bianco‐Peled

2010

J of Separation Science

View full text Add to dashboard Cite

A novel approach for enhancing protein recognition in molecularly imprinted hydrogel (MIH) is presented. This approach was developed based on the hypothesis that the number of specific binding sites created in the previously described MIH is very small, thus attempts to enhance the capacity result in most cases in additional non-specific binding and loss of selectivity. Thus, blocking the non-specific binding sites could lead to higher capacities and better selectivity. To test this hypothesis, MIH interpenetrating networks designed to block non-specific binding sites were synthesized using two separate stages of polymerization. Re-binding of the template protein (lysozyme) and a competitor protein (cytochrome C) was measured, and the results were compared with the similar experiment performed using a control non-imprinted hydrogel and a "conventional" MIH. The imprinting efficacy of the MIH interpenetrating network was found to be much higher than that of the controls. Furthermore, competitive adsorption assays have demonstrated the superiority of the new formulation.

show abstract

“…Chitosan (CS), poly(b-(1,4)-2-amino-2-deoxy-D-glucose), is a hydrophilic polyelectrolyte obtained by the deacetylation of chitin, which is very abundant in nature [19]. CS is a class of cationic polysaccharides containing large number of amine groups together with hydroxyl groups under acidic conditions [20,21]. GPTMS is one of the silane-coupling agents with an epoxy group and three methoxysilane groups [16,17].…”

Section: Introductionmentioning

confidence: 99%

Chitosan–silica hybrid‐coated open tubular column for hydrophilic interaction capillary electrochromatography

Yin

2011

J of Separation Science

View full text Add to dashboard Cite

A novel and convenient protocol for the preparation of an open-tubular column coated with chitosan-silica hybrid using chitosan and silane-coupling agent (γ-glycidoxy-propyltrimethoxysilane) was developed for CEC, in which, chitosan was covalently bonded to the inner wall of a fused-silica capillary using γ-glycidoxy-propyltrimethoxysilane as a cross-linking agent. The stationary phase was hydrophilic due to the chitosan-silica hybrid with abundant amine and hydroxyl functional groups. The chromatographic characteristics of the column were evaluated by the separation of some organic acids and inorganic anions. The column showed good selectivity for nucleotides, aromatic acids, and inorganic anions. The mechanism for the separation of these compounds was primarily based on the hydrophilic and electrostatic interactions combined with the electrophoretic mechanism. The CEC method on the column for the separation of these compounds was compared with CE method in a bare capillary.

show abstract

Hemoglobin Recognition by Imprinting in Semi-Interpenetrating Polymer Network Hydrogel Based on Polyacrylamide and Chitosan

Cited by 117 publications

References 29 publications

Swelling characterization and drug delivery kinetics of polyacrylamide-co-itaconic acid/chitosan hydrogels

Swelling characterization and drug delivery kinetics of polyacrylamide-co-itaconic acid/chitosan hydrogels

Molecularly imprinted hydrogel displaying reduced non‐specific binding and improved protein recognition

Chitosan–silica hybrid‐coated open tubular column for hydrophilic interaction capillary electrochromatography

Contact Info

Product

Resources

About