Hemoglobin Variants in Patients With Microcytic Hypochromic Anemia: A Review of Indian Studies

Kharche, Kaustubh; Bhake, Arvind

doi:10.7759/cureus.38357

Cited by 3 publications

(4 citation statements)

References 17 publications

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“…The evaluation of haemoglobin abnormalities in the form of characteristics and diseases is advised for all paediatric patients with refractory anaemia. 15 F Afshin et al (2021) did a study to concluded that the examination of Hb Bart's and Hb H are direct approaches for the diagnosis of thalassemia, however only a few cases of thalassemia were found in this study, and the most of them were missed. Because of this, -thalassemia must be confirmed in Hb Bart's newborns, and molecular investigation is required to count the number of defective genes.…”

Section: Study Statusmentioning

confidence: 56%

“…13 Kamran DS et al (2023) did a study to concluded that peripheral smear can be used in high-risk locations and, although though it is not a very sensitive tool for screening for the Sickle trait, it can be supported by more advanced methods in ambiguous circumstances. 14 Kharche K et al(2023) did a study to concluded that in order to treat microcytic hypochromic anaemia with a hemolytic component that is resistant to treatment, a systematic review of 14 research from four regions and across India was conducted. The rare haemoglobin variations that the electrophoresis was unable to identify are likely best discovered by the HPLC.…”

Section: Study Statusmentioning

confidence: 99%

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Assessing the efficacy of hemoglobin electrophoresis as a diagnostic tool in moderate to severe anemia: a study protocol

Bhongade,

Vodithala,

Vagha

2023

F1000Res

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Patients with hemoglobinopathy may experience short-term benefits from nutritional support and blood transfusions, but long-term results may be better if a precise diagnosis is obtained and a precise course of treatment or safety measures is adopted. The goal of the study is to determine the prevalence of haemoglobin abnormalities in people who presented with moderate to severe anaemia using haemoglobin electrophoresis They will undergo haemoglobin electrophoresis to test for the presence of any haemoglobin abnormalities. For anaemia classification, the WHO reference range is used. The outcome and quality of life of patients with haemoglobin disorders can be improved when they are properly managed, and since a significant portion of the patients in our study have hemoglobinopathies of varying severity, it would be prudent to conduct investigations to determine the precise cause of anaemia, particularly in rural areas where supportive management remains the mainstay of treatment in most scenarios.

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Section: Study Statusmentioning

confidence: 56%

Section: Study Statusmentioning

confidence: 99%

Assessing the efficacy of hemoglobin electrophoresis as a diagnostic tool in moderate to severe anemia: a study protocol

Bhongade,

Vodithala,

Vagha

2023

F1000Res

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“…Using a preprogrammed buffer gradient that raises the ionic strength depending on ionic interactions to the analytical cartridge, hemoglobin is separated from the analytical cartridge by cation-exchange HPLC [16]. More samples can be processed faster and with fewer technicians due to the HPLC's automation.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

“…Pan India surveillance and the systematic review carried by Kharche K, Bhake over 14 studies across four regions revealed that hemoglobinopathies must be analyzed in microcytic hypochromic anemia with a hemolytic component that is resistant to treatment. The HPLC is likely the best analyzer for finding the rare hemoglobin variants that the electrophoresis was unable to find [16]. Hbs A, A2, F, S, C, and D Punjab and G Philadelphia usually remain different from each other.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Untitled

2024

J. Med. Chem. Sci.

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The most prevalent hemoglobinopathy is sickle cell disease, which is followed by β-thalassemia and thalassemia syndrome. The Mediterranean region, Middle East, Indian subcontinent, South east Asia, and the northern coast of Africa have been found to have the highest prevalence of both diseases. The majority of affected youngsters in many of these regions pass away before turning five without ever receiving a diagnosis. Our understanding of hemoglobinopathies' clinical and therapeutic features as well as their laboratory diagnosis has increased as a result of several study articles. Erythrocyte index, hemoglobin analysis by HPLC and capillary electrophoresis, and recently employed next-generation sequencing (NGS) to screen for thalassemia are the main biochemical and molecular procedures used in clinical laboratories. Preventive treatment and genetic counselling can be performed when SCD is detected and diagnosed early, which helps to lower mortality and minimize sequelae. Current diagnosis procedures are laboratory-based and call for expensive diagnostic tools and qualified employees. India has a vast population, making it impossible for them to pay for traditional diagnostics. The primary benefit of POCT is its low cost, and its effect on hemoglobinopathies may ultimately contribute to the improvement of India's health situation and other relevant fields.

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Assessment and comparison of the sensitivity and specificity of sickling and haemoglobin electrophoresis in haemolytic anaemia patients: A study protocol

Mungale,

Singh Chauhan,

Jagtap

2023

F1000Res

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The present protocol will compare haemoglobin electrophoresis with sickling test, evaluating sensitivity, specificity, positive predicted value and negative predicted value of haemolytic anaemia patients. Haemoglobin electrophoresis is the confirmation test for sickle cell anaemia. Sickle cell anaemia is a type of haemolytic anaemia where red blood cells (RBC)’s biconcave form does not enable the best possible respiratory exchange. In haemolytic anaemia, EDTA is used to examine the peripheral smear stained with Giemsa stain. Peripheral smear shows polychromatic neutrophils, nucleated RBCs (Red blood cell) and fragmented RBCs. In haemolytic anaemia, patients have decreased haemoglobin (Hb) levels. In sickle cell disease (SCD), anaemia is brought about by a change in the DNA sequence of cells in the Hb beta-globin chain. Screening of the sickling test is done by using sodium metabisulfite to assess the shape of cells. Confirmation test of Hb electrophoresis on cellulose acetate paper uses band detection. This study will aim to evaluate the sensitivity and specificity of Hb electrophoresis with the sickling test utilizing quantifiable investigation. The effectiveness of sensitivity and specificity, positive and negative predicted values, and overall course of action for the two techniques, Hb electrophoresis and sickling test, will be collected. This research will also give an overview of existing haemolytic anaemia literature.

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Hemoglobin Variants in Patients With Microcytic Hypochromic Anemia: A Review of Indian Studies

Cited by 3 publications

References 17 publications

Assessing the efficacy of hemoglobin electrophoresis as a diagnostic tool in moderate to severe anemia: a study protocol

Assessing the efficacy of hemoglobin electrophoresis as a diagnostic tool in moderate to severe anemia: a study protocol

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Assessment and comparison of the sensitivity and specificity of sickling and haemoglobin electrophoresis in haemolytic anaemia patients: A study protocol

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