Hemolymph: Composition

Florkin, Marcel; Jeuniaux, Charles

doi:10.1016/b978-0-12-591605-9.50013-5

Cited by 85 publications

(51 citation statements)

References 184 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…females will not usually both feed and lay an egg in the same host), hosts are found in patches (several hosts per infected bean, groups of beans) and thus feeding and reproductive opportunities are likely to be correlated. The nutritional ecology of this species allows us to make two speci¢c predictions about its pattern of allocation of larval reserves: ¢rst, given the delay in the appearance of nutrients in the eggs after the initial host meal, larval reserves should be the main source of nutrients for egg production for four or ¢ve days after emergence, and second, given the frequent host-feeding events, the high nutrient content of a haemolymph meal (Florkin & Jeuniaux 1964;Mullins 1985) and the ability of the female to store amino acids for future reproductive purposes (Rivero & Casas 1999b), larval reserves should not be invested in egg production at any other point of the female life span but should be saved to prolong survival in the event of host deprivation. We discuss the use of larval reserves in the context of the speci¢c life history and nutritional requirements of this species and compare our results with those found in butter£ies and moths (Boggs 1997a;O'Brien et al 2000).…”

Section: Introductionmentioning

confidence: 99%

Lifetime allocation of juvenile and adult nutritional resources to egg production in a holometabolous insect

Rivero

Giron

Casas

2001

Proc. R. Soc. Lond. B

View full text Add to dashboard Cite

In holometabolous insects reproductive success is strongly determined by the nutritional resources available to the females. In addition to nutrients derived from adult feeding, resources for egg production may come from the limited reserves accumulated during the larval stages. The pattern of allocation of these larval reserves to egg production is expected to be strongly linked to the nutritional ecology of the adult. We investigate the temporal pattern of allocation of larval reserves to reproduction in a hostfeeding parasitoid wasp. As predicted by the dynamics of allocation of an adult meal, larval reserves are the main source of nutrients for four or ¢ve days after emergence. However, despite the high frequency of host feeding and the high nutrient content of a haemolymph meal, which we predicted would lead to larval reserves being conserved in the event of host deprivation, larval reserves contribute to egg production throughout the lifetime of the female. We propose several mechanistic and adaptive explanations for our results, including the possible existence of a limiting or key nutrient for egg production of exclusively larval origin. We make further predictions concerning the pattern of allocation of larval resources in parasitoids with di¡erent adult nutritional requirements.

show abstract

Section: Introductionmentioning

confidence: 99%

Lifetime allocation of juvenile and adult nutritional resources to egg production in a holometabolous insect

Rivero

Giron

Casas

2001

Proc. R. Soc. Lond. B

View full text Add to dashboard Cite

show abstract

“…INSECTS are known to contain high levels of free amino acids in their haemolymph and tissues (FLORKIN and JEUNIAUX, 1974;SACKTOR, 1965). The physiological function of most of these amino acids is still not fully understood.…”

Section: Introductionmentioning

confidence: 99%

Proline synthesis in fat body of Leptinotarsa decemlineata

Weeda

Koopmanschap

Kort

et al. 1980

Insect Biochemistry

View full text Add to dashboard Cite

show abstract

“…In spite of its inability to import these two sugars, the crr-disrupted mutant GII3-gt1 multiplied in the leafhopper vector and was transmitted to periwinkle plants, in which it induced symptoms. In insects, trehalose is the main sugar in the haemolymph but glucose and fructose also are present (Florkin & Jeuniaux, 1974). Therefore, it is likely that multiplication of GII3-gt1 in the leafhopper vector mainly relies on the use of fructose.…”

Section: Discussionmentioning

confidence: 99%

Specific gene targeting in Spiroplasma citri: improved vectors and production of unmarked mutations using site-specific recombination

2005

View full text Add to dashboard Cite

In Spiroplasma citri, where homologous recombination is inefficient, specific gene targeting could only be achieved by using replicative, oriC plasmids. To improve the probability of selecting rare recombination events without fastidious, extensive passaging of the transformants, a new targeting vector was constructed, which was used to inactivate the crr gene encoding the IIA component of the glucose phosphotransferase system (PTS) permease. Selection of recombinants was based on a two-step strategy using two distinct selection markers, one of which could only be expressed once recombination had occurred through one single crossover at the target gene. According to this strategy, spiroplasmal transformants were screened and multiplied in the presence of gentamicin before the crr recombinants were selected for their resistance to tetracycline. In contrast to the wild-type strain GII-3, the crr-disrupted mutant GII3-gt1 used neither glucose nor trehalose, indicating that in S. citri the glucose and trehalose PTS permeases function with a single IIA component. In addition, the feasibility of using the transposon cd TnpR/res recombination system to produce unmarked mutations in S. citri was demonstrated. In an arginine deiminase (arcA-disrupted) mutant, the tetM gene flanked by the res sequences was efficiently excised from the chromosome through expression of the TnpR resolvase from a replicative oriC plasmid. Due to oriC incompatibility, plasmid loss occurred spontaneously when selection pressure was removed. This approach will be helpful for constructing unmarked mutations and generating multiple mutants with the same selection marker in S. citri. It should also be relevant to other species of mollicutes.

show abstract

Hemolymph: Composition

Cited by 85 publications

References 184 publications

Lifetime allocation of juvenile and adult nutritional resources to egg production in a holometabolous insect

Lifetime allocation of juvenile and adult nutritional resources to egg production in a holometabolous insect

Proline synthesis in fat body of Leptinotarsa decemlineata

Specific gene targeting in Spiroplasma citri: improved vectors and production of unmarked mutations using site-specific recombination

Contact Info

Product

Resources

About