Hemolysis and zoological relationship. Comparative studies with four penetrating non‐electrolytes

Jacobs, M. H.; Glassman, Harold N.; Parpart, Arthur K.

doi:10.1002/jez.1401130203

Cited by 66 publications

(13 citation statements)

References 15 publications

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“…They reported an average value for the thermodynamic form of the permeability coefficient, w, of 13 x 10~1 5 moles/ dyne cm" 1 , which corresponds to a &,' value of 320 x 10~6 cm/sec. This value is of the same order of magnitude as that which we found for dog red cells, as expected from the similar hemolysis times in these species (5). The ratio of the permeability of the dog red cells to urea and thiourea is also of the order expected on the basis of their differing hemolysis times (5).…”

Section: Conversion Of Our Rate Constants For Red Cellsupporting

confidence: 86%

“…This value is of the same order of magnitude as that which we found for dog red cells, as expected from the similar hemolysis times in these species (5). The ratio of the permeability of the dog red cells to urea and thiourea is also of the order expected on the basis of their differing hemolysis times (5). The permeability coefficient that we obtained for chloride can be compared with that which arises from the data obtained by Tosteson (10).…”

Section: Conversion Of Our Rate Constants For Red Cellsupporting

confidence: 84%

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Red cell carriage of label: its limiting effect on the exchange of materials in the liver.

Goresky¹,

Bach²,

Nadeau³

1975

Circulation Research

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The red cell membrane is a permeability barrier that limits the equilibration of a variety of solutes between red cell and plasma water. We utilized the multiple indicator dilution technique to investigate the effect of this barrier on the exchange in the liver of a group of tracer substances that are not removed in net fashion from the hepatic circulation: thiourea, urea, and chloride. We demonstrated that, after preequilibration of the label with red cells, a red cell carriage effect appeared (the trapping and translocation of label in the red cells), that this effect was most marked when the permeability of the red cell was relatively low for the substance under consideration (thiourea), and that the effect became small when the permeability of the red cells was large for the exchanging substance (urea and chloride). We developed a theoretical description of the retarding effect of the red cell permeability barrier on the extravascular exchange of label and were able to use this description to obtain estimates of the red cell permeability from the in vivo dilution curves. We examined the effect of plasma injection, of changing the input in such a fashion that the label was not preequilibrated with red cells, and found, both experimentally and theoretically, that for substances of low permeability the transit time from these experiments, if multiplied by the total water flow or solute flux, gave an overestimate of both the apparent total volume of distribution and the mass of traced material in the system. This last effect is of great importance for the practical design of many biological experiments. Reliable volume and mass estimates can be made only when the labeled material has been preequilibrated with red cells. KEY WORDSred cell permeability thiourea urea and chloride multiple indicator dilution studies hepatic microcirculation theoretical estimates of volume and mass dog red blood cells permeability barrier of the red cell membrane• The membrane of the red blood cell constitutes a permeability barrier that is significant for a variety of low molecular weight substances. When tracer is added to blood, this barrier limits the rate of equilibration of label between the red cells and the plasma. Through this effect, the barrier changes the manner in which the label is distributed into tissue during the blood's passage through the microcirculatory bed of an organ. In similar fashion, if there is net removal of material by the organ, the presence of the red cell permeability barrier limits the accessibility of the material within the red cells to the removal mechanism. The phenomena which result from limited permea- 328bility across the red cell membrane have not been extensively explored in a quantitative fashion in mammalian systems. Our purpose is to provide such an exploration.We selected the liver as the organ most suitable for this exploration. The hepatic parenchymal cells are rather more permeable to low molecular weight substances than are the red cells, and this permeability difference pro...

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Section: Conversion Of Our Rate Constants For Red Cellsupporting

confidence: 86%

Section: Conversion Of Our Rate Constants For Red Cellsupporting

confidence: 84%

Red cell carriage of label: its limiting effect on the exchange of materials in the liver.

Goresky¹,

Bach²,

Nadeau³

1975

Circulation Research

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“…This hypothesis has been strengthened by Kdgl, De Gier, Mulder, and van Deenen (55) and by De Gier and van Deenen (26), who demonstrated differences in the fatty acid composition and phospholipid distribution of erythrocyte membranes in various mammalian species. These variations could be correlated with the species differences in erythrocyte permeability to ethylene glycol and glycerol previously reported by Jacobs, Glassman, and Parpart (56). It was concluded, therefore, that membrane lipid composition might be an important determinant of membrane permeability (26,55).…”

Section: Discussionsupporting

confidence: 57%

Human Fetal Erythrocyte and Plasma Lipids*

Crowley¹,

Ways²,

Jones³

1965

J. Clin. Invest.

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“…Inspired by earlier works (Jacobs, 1931;Jacobs et al, 1950), the present study, in combination with previous studies Brahm, 1977;Brahm, 1982;Brahm, 1983b), compares chloride, urea and water permeability of RBC from chicks, ducks, salamanders (Amphiuma means), dogs and humans. Both previous and present results were obtained with the same techniques and are, therefore, directly comparable.…”

Section: Introductionmentioning

confidence: 93%

“…The Journal of Experimental Biology 216 (12) different species transport solutes differently (Jacobs, 1931;Jacobs et al, 1950), and a proper selection of RBC species may reveal whether urea and water share a pathway in common in RBC. The selection of RBC species reflects that chicks and ducks, like other birds, excrete uric acid and that their RBC have no UT-B.…”

Section: Do Urea and Water Share A Pathway In Common In Rbc?mentioning

confidence: 99%

The permeability of red blood cells to chloride, urea, and water

Brahm

2013

Journal of Experimental Biology

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SUMMARYThis study extends permeability (P) data on chloride, urea and water in red blood cells (RBC), and concludes that the urea transporter (UT-B) does not transport water. cms -1 (human). DIDS, DNDS and phloretin inhibit P Cl by >99% in all RBC species. PCMBS, PCMB and phloretin inhibit P urea by >99% in Amphiuma, dog and human RBC, but not in chick and duck RBC. PCMBS and PCMB inhibit P d in duck, dog and human RBC, but not in chick and Amphiuma RBC. Temperature dependence, as measured by apparent activation energy, E A , of P Cl is 117.8 (duck), 74.9 (Amphiuma) and 89.6kJmol −1 (dog). The E A of P urea is 69.6 (duck) and 53.3kJmol −1 (Amphiuma), and that of P d is 34.9 (duck) and 32.1kJmol −1 (Amphiuma). The present and previous RBC studies indicate that anion (AE1), urea (UT-B) and water (AQP1) transporters only transport chloride (all species), water (duck, dog, human) and urea (Amphiuma, dog, human), respectively. Water does not share UT-B with urea, and the solute transport is not coupled under physiological conditions.

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Hemolysis and zoological relationship. Comparative studies with four penetrating non‐electrolytes

Cited by 66 publications

References 15 publications

Red cell carriage of label: its limiting effect on the exchange of materials in the liver.

Red cell carriage of label: its limiting effect on the exchange of materials in the liver.

Human Fetal Erythrocyte and Plasma Lipids*

The permeability of red blood cells to chloride, urea, and water

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