Hemolytic Activity of a Togavirus, Getah*

Abstract

A purified toga-alphavirus, Getah (GE T), showed optimal hemolytic activity for one-day-old chick red blood cells when incubated at 37 C for 120 min at pH 6.2. Experimental data obtained from various angles, such as pH dependency, inhibition by virus-specific antiserum and by concanavalin A, indicated that the hemolysis was a property of the virus particle itself. Although the mechanism of hemolysis by togaviruses has not been known, our results indicated that viral lipids may participate in this activity since the hemolytic activity was impaired by delipidation procedures.

Monoclonal Antibodies to Sindbis Virus Glycoprotein E1 can Neutralize, Enhance Infectivity, and Independently Inhibit Haemagglutination or Haemolysis

“…Biological functions associated with the surface of the virus include neutralization of infectivity, haemagglutination (Clarke & Casals, 1958) and haemolysis (Karabatsos, 1963(Karabatsos, , 1965(Karabatsos, , 1973Goto et al, 1979;V~i~in~inen & K~i~iri~iinen, 1980;Yoshinaka et al, 1978).…”

Hemolysis-inhibition test for detection of rubella antibody

“…They include Newcastle disease (6), SendM (2), measles (12), Getah (17), Semliki Forest (15,16), Sindbis (i5, 16), Akabane (3) and eastern and western equine encephalitis viruses (5). One of the present authors, KO~AYASHI (7), demonstrated hemolytic activity for rubella virus by chelating calcium ion with ethylene-diamine-tetraacetie acid (EDTA) after adsorption of the virus to chicken erythroeytes in the presence of the ion.…”