Hemolytic-uremic syndrome and enterohemorrhagic Escherichia coli

Lk, Pickering; Tg, Obrig; Fb, Stapleton

doi:10.1097/00006454-199406000-00001

Cited by 86 publications

(77 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Epidemiologic data suggest that isolates producing Stx2 alone are more likely to cause severe disease than those producing only Stx1 or a combination of Stx1 and Stx2 (3,41,44). Evidence of a role for Stx in the development of severe EHECrelated disease comes from several studies using animal models.…”

mentioning

confidence: 99%

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

et al. 2003

View full text Add to dashboard Cite

Enterohemorrhagic Escherichia coli (EHEC) is a group of food-borne pathogens that can cause diarrhea, colitis, and the hemolytic uremic syndrome (HUS). The importance of several of the proposed EHEC virulence factors lacks experimental verification in animal models. The limitations of current animal models led us to reexamine the infant rabbit model for the study of EHEC pathogenicity. Here, we report that intragastric inoculation of a Shiga toxin 2 (Stx2)-producing E. coli O157:H7 clinical isolate into infant rabbits led to severe diarrhea and intestinal inflammation but no signs of HUS. We constructed a set of isogenic derivatives of this isolate with deletions in several putative virulence genes, including stx 2 , eae, tir, and ehxA, to investigate the contribution of individual virulence factors to EHEC pathogenicity. stx 2 increased the severity and duration of EHEC-induced diarrhea. Furthermore, although stx 2 had no role in EHEC intestinal colonization nor was it required for EHEC-induced inflammation, stx 2 altered how the host responded to EHEC infection by promoting heterophilic infiltration of the colonic epithelium and lamina propria. Intragastric inoculation of purified Stx2 also induced inflammation and diarrhea in this model. Diarrhea and intestinal inflammation were also dependent on EHEC colonization, as EHEC derivatives with deletions in eae and tir did not colonize, form attaching and effacing lesions, or develop clinical signs of disease. Our studies indicate that infant rabbits are a useful model for investigation of the intestinal stage of EHEC pathogenesis and suggest that Shiga toxin may play a critical role in causing diarrhea and inflammation in patients infected with EHEC.

show abstract

mentioning

confidence: 99%

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Tout d'abord, les infections à E. coli entéro-hémorragique (sérotype 0157:H7) et à Shigella dysenteriae peuvent provoquer des flambées, des sortes de petites épidémies, de SHU chez les enfants en bas âge [18,19]. Ensuite, d'autres germes comme le Streptococcus pneumoniae peuvent aussi être incriminés.…”

Section: Purpura Thrombopénique Thrombotique (Ptt) Et Syndrome Hémolyunclassified

Diagnostic étiologique des anémies, 2e partie

Peghini¹,

Fehr²

2002

Forum Med Suisse

View full text Add to dashboard Cite

“…Shiga toxin-producing Escherichia coli (STEC) is an emerging bacterial pathogen responsible for food-borne outbreaks of disease around the world, resulting in significant morbidity and mortality, including hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS) (Pickering et al 1994;Paton and Paton 1998). Shiga toxins and ricin, produced by the castor bean (Ricinus communis) are of major concern for public health and bioterrorism and have been classified as category B select agents.…”

Section: Introductionmentioning

confidence: 99%

Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model

Pierce

Kahn²,

Chiou³

et al. 2010

RNA

View full text Add to dashboard Cite

Ricin produced by the castor bean plant and Shiga toxins produced by pathogenic Escherichia coli (STEC) and Shigella dysenteriae are type II ribosome inactivating proteins (RIPs), containing an enzymatically active A subunit that inhibits protein synthesis by removing an adenine from the a-sarcin/ricin loop (SRL) of the 28S rRNA. There are currently no known antidotes to Shiga toxin or ricin, and the ability to screen large chemical libraries for inhibitors has been hindered by lack of quantitative assays for catalytic activity that can be adapted to a high throughput format. Here, we describe the development of a robust and quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay that can directly measure the toxins' catalytic activity on ribosomes and can be used to examine the kinetics of depurination in vivo. The qRT-PCR assay exhibited a much wider dynamic range than the previously used primer extension assay (500-fold vs. 16-fold) and increased sensitivity (60 pM vs. 0.57 nM). Using this assay, a 400-fold increase in ribosome depurination was observed in yeast expressing ricin A chain (RTA) relative to uninduced cells. Pteroic acid, a known inhibitor of enzymatic activity, inhibited ribosome depurination by RTA and Shiga toxin 2 with an IC 50 of~100 mM, while inhibitors of ricin transport failed to inhibit catalytic activity. These results demonstrate that the qRT-PCR assay would enable refined kinetic studies with RIPs and could be a powerful screening tool to identify inhibitors of catalytic activity.

show abstract

Hemolytic-uremic syndrome and enterohemorrhagic Escherichia coli

Cited by 86 publications

References 0 publications

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

Diagnostic étiologique des anémies, 2e partie

Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model

Contact Info

Product

Resources

About

Hemolytic-uremic syndrome and enterohemorrhagic Escherichia coli

Cited by 86 publications

References 0 publications

Critical Roles for stx 2 , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

Critical Roles for stx 2 , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

Diagnostic étiologique des anémies, 2e partie

Development of a quantitative RT-PCR assay to examine the kinetics of ribosome depurination by ribosome inactivating proteins using Saccharomyces cerevisiae as a model

Contact Info

Product

Resources

About

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits

Critical Roles for stx ₂ , eae , and tir in Enterohemorrhagic Escherichia coli -Induced Diarrhea and Intestinal Inflammation in Infant Rabbits