Hemopoiesis in human fetal and embryonic liver

Timens, Wim; Kamps, Willem A.

doi:10.1002/(sici)1097-0029(19971201)39:5<387::aid-jemt1>3.0.co;2-e

Cited by 45 publications

(25 citation statements)

References 49 publications

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“…The question remains whether CD43 expression on hematopoietic progenitors before CD45 seen during hESC differentiation in vitro recapitulate events occurring in vivo. CD43 ϩ CD45 Ϫ Lin Ϫ hematopoietic blast cells morphologically similar to hESC-derived CD43 ϩ CD45 Ϫ Lin Ϫ hematopoietic progenitors have been described in human embryonic and fetal liver, 55 suggesting that these cells are an integral part of hematopoietic development in vivo.…”

Section: Discussionmentioning

confidence: 99%

Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures

Vodyanik

Thomson

Slukvin

2006

Blood

310

344

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Section: Discussionmentioning

confidence: 99%

Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures

Vodyanik

Thomson

Slukvin

2006

Blood

310

344

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“…Morphological and immunohistochemical analysis of fetal liver reveals myelopoiesis mainly around the blood vessels, that is, in places where the fibroblasts and myofibroblasts are located [8,36]. These findings may reflect their important role in the regulation of myeloid differentiation.…”

Section: Fibroblasts and Myofibroblastsmentioning

confidence: 92%

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Payushina

2012

ISRN Cell Biology

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Hematopoiesis is the main function of the liver during a considerable period of mammalian prenatal development. Hematopoietic cells of the fetal liver exist in a specific microenvironment that controls their proliferation and differentiation. This microenvironment is created by different cell populations, including epitheliocytes, macrophages, various stromal elements (hepatic stellate cells, fibroblasts, myofibroblasts, vascular smooth muscle and endothelial cells, mesenchymal stromal cells), and also cells undergoing epithelial-to-mesenchymal transition. This paper considers the involvement of these cell types in the regulation of fetal liver hematopoiesis.

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“…In the human hematopoietic system, WT1 appears to be normally expressed in bone marrow CD34 ϩ cells. 2,20,[42][43][44][45][46][47] WT1 mRNA levels are modulated during the in vitro differentiation of hematopoietic cells and leukemic cells lines. 23,44,48,49 In vitro manipulation of WT1 expression has been shown to affect the growth potential and differentiation of hematopoietic cells, leukemia cells, and cell lines.…”

Section: Introductionmentioning

confidence: 99%

Role of the WT1 tumor suppressor in murine hematopoiesis

Alberta

Springett

Rayburn

et al. 2003

Blood

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The WT1 tumor-suppressor gene is expressed by many forms of acute myeloid leukemia. Inhibition of this expression can lead to the differentiation and reduced growth of leukemia cells and cell lines, suggesting that WT1 participates in regulating the proliferation of leukemic cells. However, the role of WT1 in normal hematopoiesis is not well understood. To investigate this question, we have used murine cells in which the WT1 gene has been inactivated by homologous recombination. We have found that cells lacking WT1 show deficits in hematopoietic stem cell function. Embryonic stem cells lacking WT1, although contributing efficiently to other organ systems, make only a minimal contribution to the hematopoietic system in chimeras, indicating that hematopoietic stem cells lacking WT1 compete poorly with healthy stem cells. In addition, fetal liver cells lacking WT1 have an approximately 75% reduction in erythroid blastforming unit (BFU-E), erythroid colonyforming unit (CFU-E), and colony-forming unit-granulocyte macrophage-erythroidmegakaryocyte (CFU-GEMM). However, transplantation of fetal liver hematopoietic cells lacking WT1 will repopulate the hematopoietic system of an irradiated adult recipient in the absence of competition. We conclude that the absence of WT1 in hematopoietic cells leads to functional defects in growth potential that may be of consequence to leukemic cells that have alterations in the expression of WT1. IntroductionWT1 was isolated as a tumor-suppressor gene on the basis of its deletion in a subset of patients with Wilms tumor, a pediatric cancer of the kidney. 1 In addition to its mutation in 5% to 10% of Wilms tumor patients, mutations of WT1 have been implicated in a variety of other cancers including mesotheliomas, desmoplastic round cell tumors, and leukemias. 1 WT1 encodes a protein of 52 to 54 kD, containing 4 zinc fingers of the Cys2 His2 class, that has been demonstrated to act as transcription factor. 1 Potential transcriptional targets for WT1 include a variety of growth factor and growth factor receptor genes. 1 An additional role for WT1 in RNA processing has also been proposed. 1 Many leukemias, including up to 80% of acute myeloid leukemias (AMLs), have been demonstrated to express WT1. [2][3][4][5][6][7][8][9][10] Mutations of WT1 have been found in approximately 5% to 10% of AMLs, similar to the level seen in Wilms tumors, and sporadically in a variety of other leukemias. [11][12][13][14][15][16][17][18] Manipulation of WT1 expression in leukemia cells results in alterations in differentiation and growth potential. [19][20][21][22][23][24][25][26][27][28] In the treatment of leukemia, expression of WT1 in the peripheral blood and bone marrow has been suggested to be an indicator of clinical relapse. Attempts have been made to correlate WT1 expression with progression of disease and prognosis in several types of hematologic malignancies. 3,[29][30][31][32][33][34][35][36][37] The clinical use of anti-WT1 cytotoxic T-lymphocytes (CTLs) for preferential destruction of WT1-expre...

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Hemopoiesis in human fetal and embryonic liver

Cited by 45 publications

References 49 publications

Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures

Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures

Hematopoietic Microenvironment in the Fetal Liver: Roles of Different Cell Populations

Role of the WT1 tumor suppressor in murine hematopoiesis

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