Hemorrhagic Shock Resuscitation with a Low Molecular Weight Starch Reduces Neutrophil-Endothelial Interactions and Vessel Leakage in vivo

Pascual, José L.; Ferri, Lorenzo; Chaudhury, Prosanto; Seely, Andrew J. E.; Campisi, Giuseppina; Giannias, Betty; Evans, David C.; Christou, Nicolas V.

doi:10.1089/10962960152813313

Cited by 14 publications

(13 citation statements)

References 73 publications

(72 reference statements)

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“…This is consistent with other reports on improved microvascular permeability (7,9,17) and other indirect parameters of a microcirculatory improvement, such as the restored tissue oxygenation (2, 7) after HES treatment. Information is also available concerning the better efficacy of HES in comparison with GEL or DEX in influencing these parameters (24,25).…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Effects of colloid solutions on ischemia-reperfusion-induced periosteal microcirculatory and inflammatory reactions: Comparison of dextran, gelatin, and hydroxyethyl starch*

Varga

Török

Szabó

et al. 2008

Critical Care Medicine

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Section: Discussionsupporting

confidence: 93%

“…It has also been suggested that colloid solutions affect PMN adherence directly, by influencing the adhesion molecule expression independently of microhemodynamic changes (17). However, microhemodynamics and leukocyte adhesion are interrelated phenomena.…”

Section: Discussionmentioning

confidence: 99%

Effects of colloid solutions on ischemia-reperfusion-induced periosteal microcirculatory and inflammatory reactions: Comparison of dextran, gelatin, and hydroxyethyl starch*

Varga

Török

Szabó

et al. 2008

Critical Care Medicine

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“…Other investigators have also shown similar results in an in vivo, intravital microscopic model of hemorrhagic shock in which PMN rolling events in transilluminated cremaster muscle were shown to decrease in association with HES administration. (20).…”

Section: Discussionmentioning

confidence: 99%

“…However, despite this evidence of increased PMN activity, other studies have demonstrated that HES infusion after hemorrhage does not adversely affect cell-mediated immune functions, and may produce salutary effects on macrophage functions (18,19). Additionally, low-molecularweight colloids have been shown to reduce PMN-endothelial interactions and vessel leakage after hemorrhagic shock (20). It remains unclear how these conflicting findings relate to the functional state of the PMN.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyethyl Starch Inhibits Neutrophil Adhesion and Transendothelial Migration

Handrigan¹,

Burns

Donnachie³

et al. 2005

Shock

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A resuscitation strategy that significantly alters the state of neutrophil (PMN) activation may impact organ function and survivability after shock. Various resuscitative fluids have been shown to elicit a severe immune activation and an upregulation of cellular injury markers, whereas other fluids have been shown to be protective. Recent studies have demonstrated that hydroxyethyl starch (HES), an artificial colloid, may exert significant anti-inflammatory effects, whereas conflicting studies with the same substance have shown an increase in PMN activation. Successful manipulation of the early immune events associated with hemorrhage and resuscitation will require a better understanding of the possible pro- or anti-inflammatory effects of resuscitation fluids. Our study investigated the effect of HES directly on PMN and cultured vascular endothelial cells in vitro. The effect of HES on PMN surface expression of CD11b and L-selectin was measured by flow cytometry. PMN activation response to HES was measured using a shape-change assay in response to formyl-methionyl-leucyl-phenylalanine (f-MLP). The effect of HES on endothelial cell surface expression of E-selectin, P-selectin, vascular cell adhesion molecule-1(VCAM-1), and intracellular adhesion molecule-1 (ICAM-1) was evaluated by enzyme-linked immunoabsorbant assay. PMN rolling, adhesion, and migration events were measured using direct microscopy under conditions simulating microvascular flow. PMN surface expression of CD11b and L-selectin in whole blood samples and isolated PMNs were unaffected by exposure to HES. HES had no effect on the normal f-MLP dose-dependent increase in PMN activation. In the absence of IL-1 stimulation, there was a small but statistically significant (P < 0.05) increase in ICAM-1 after exposure to HES. After stimulation with IL-1 (10 U/mL), HES had no effect on the expression of P-selectin, E-selectin, ICAM-1, or VCAM-1. Under simulated microvascular flow conditions in vitro, HES significantly diminished the PMN tethering rate (P < 0.05) and the transendothelial migration rate (P < 0.05) in a dose-dependent manner. HES significantly alters the function of the PMN at the interface of the PMN responding to activated endothelium. The effect occurs, surprisingly, without a coincident effect on the state of PMN activation or a significant change in the surface expression of the adhesion molecules responsible for PMN-endothelial interaction.

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“…Inappropriate cellular activation and their vigorous interaction with the endothelial lining are responsible for local and systemic release of toxic mediators, in particular reactive oxygen species (ROS) that cause vessel injury and leakage. When different methods to determine permeability changes and estimate fluid sequestration and organ edema were used, the application of hypertonic saline in various models of organ damage revealed less extravasation and leakage than did normotonic crystalloids [44][45][46][47]. Newer crystalloids, such as ethyl pyruvate solution, might even be superior to hypertonic saline because of their unique ROS scavenging action [48] and anti-inflammatory property [49].…”

Section: Cellular Effects Of Hypertonic Crystalloidsmentioning

confidence: 99%

Volume replacement and microhemodynamic changes in polytrauma

Vollmar

Menger

2004

Langenbecks Arch Surg

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Though fluid administration is one of the most basic concepts in resuscitation, there is ongoing controversy and continuing research on the definition of the ideal fluid for resuscitation of trauma and hemorrhage and for intraoperative volume support. In general, crystalloids and colloids, as well as blood, blood substitutes and oxygen therapeutics, are available. This report briefly revisits the physiological mechanisms underlying resuscitation with crystalloids and colloids, emphasizing colloid-supplemented resuscitation with hypertonic saline. Finally, potential applications of oxygen therapeutics are briefly considered.

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Hemorrhagic Shock Resuscitation with a Low Molecular Weight Starch Reduces Neutrophil-Endothelial Interactions and Vessel Leakage in vivo

Abstract: 10% pentastarch reduces RL-associated EC-PMN interactions and vessel leakage following hemorrhagic shock. These results support the use of low MW starches to resuscitate hemorrhagic shock, potentially reducing PMN-mediated tissue injury.

Cited by 14 publications

References 73 publications

Effects of colloid solutions on ischemia-reperfusion-induced periosteal microcirculatory and inflammatory reactions: Comparison of dextran, gelatin, and hydroxyethyl starch*

Effects of colloid solutions on ischemia-reperfusion-induced periosteal microcirculatory and inflammatory reactions: Comparison of dextran, gelatin, and hydroxyethyl starch*

Hydroxyethyl Starch Inhibits Neutrophil Adhesion and Transendothelial Migration

Volume replacement and microhemodynamic changes in polytrauma

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