Hemosiderin Estimations and Sideroblast Counts in the Differential Diagnosis of Iron Deficiency and other Anemias

Hansen, H. A.; Weinfeld, Aleksander

doi:10.1111/j.0954-6820.1959.tb14509.x

Cited by 72 publications

(20 citation statements)

References 33 publications

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“…Histology of explants The tibia and IVDs were consecutively sectioned (5- to 7-μm sections), serially numbered, and every tenth to eleventh section was stained for orientation with hematoxylin and for Fe-labeled cells by using standard protocols [56,57]. …”

Section: Methodsmentioning

confidence: 99%

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Similar cellular migration patterns from niches in intervertebral disc and in knee-joint regions detected by in situ labeling: an experimental study in the New Zealand white rabbit

et al. 2013

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IntroductionPotential stem cell niches (SNs) were recently reported in intervertebral discs (IVDs) and knee joints (KJs) in different mammals (located adjacent to the epiphyseal plate; EP). The aim here was to examine further possible cellular migration and migration directions of cells originating from niches possibly involved in regeneration of cartilaginous tissues in the IVD and in the KJ regions in adult mammals.MethodsIn total, 33 rabbits were used in studies A through C.A. IVD cells were sorted; fluorescence-activated cell sorting (FACS) by size (forward scatter; ≤10 μm or >10 μm or GDF5+ cells (anti-GDF5 antibody). Sorted cells, labeled with cell tracer (carboxyfluorescein-diacetate-succinimidyl ester; CDFA-SE) were applied on IVD explants in vitro. Migrating cells/distance was evaluated by fluorescence- and confocal-microscopy (FC).B. DNA labeling was performed with BrdU (oral administration). Animals were killed (14 to 56 days), KJs collected, and BrdU+ cells visualized with immunohistochemistry (IHC)/anti-BrdU antibody in SN and articular cartilage (AC).C. Cell tracer: (Fe-nanoparticles: Endorem) were injected into SNs of IVDs (LI-LV) and KJs (tibia). Animals were killed after 2 to 6 weeks. Fe-labeled cells were traced by ferric-iron staining (Prussian blue reaction; Mallory method).ResultsA. GDF5+ cells and ≤10-μm cells displayed the best migration capability in IVD explants. GDF5+ cells were detected at a tissue depth of 1,300 μm (16 days). B. BrdU+ cells were observed in early time points in niches of KJs, and at later time points in AC, indicating a gradual migration of cells. C. Fe+ cells were detected in IVDs; in annulus fibrosus (AF) in 11 of 12 animals and in nucleus pulposus (NP) in two of 12 animals. In AC (tibia), Fe+ cells were detected in six of 12 animals. In the potential migration route (PMR), from niches toward the IVD, Fe+ cells (three of 12 animals) and in PMR toward AC (KJs) (six of 12 animals) were detected.ConclusionsResults indicate similar cellular migration patterns in cartilage regions (IVD and KJs) with migration from stem cell niche areas into the mature cartilaginous tissues of both the KJs and the IVD. These findings of a cellular migration pattern in mature cartilage are of interest from tissue-repair and engineering perspectives.

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Section: Methodsmentioning

confidence: 99%

“…Tracing of migrating Fe-labeled cells Detection of Fe-labeled cells was performed in the sections by using standard Fe-staining protocols with the Prussian blue reaction (Mallory method) [57]. More than two observations of Fe-labeled cells per organ was considered a positive result for each animal.…”

Section: Methodsmentioning

confidence: 99%

Similar cellular migration patterns from niches in intervertebral disc and in knee-joint regions detected by in situ labeling: an experimental study in the New Zealand white rabbit

et al. 2013

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“…The methods used for the determination of Hb concentration, Hct, serum iron, total iron binding capacity (TIBC), blood volume and total Hb mass, desferrioxaniine (DF)-induced urinary iron excretion, were the same as previously described (29). Bone marrow smears were stained for hemosiderin according to the method of Hansen and Weinfeld (15).…”

Section: Methodsmentioning

confidence: 77%

Availability of Iron Dextran for Hemoglobin Synthesis

Olsson

Weinfeld

1972

Journal of Internal Medicine

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The availability of iron dextran for hemoglobin formation has been studied in six young, healthy male volunteers made anemic and iron depleted by repeated phlebotomy. A quantity of 1 850 mg iron dextran was infused calculated to restore not only the Hb deficit but also the iron stores. The rate of Hb production from iron dextran was highest during the first week following infusion and found to be of the same order as in a control group in six males receiving iron orally. However, from the second week on, there was a significantly slower Hb regeneration in the iron dextran group than in controls, indicating a restricted delivery of iron from the iron dextran complex to the erythroid marrow. Three to five months after infusion five of the subjects receiving iron dextran were subjected to a new series of phlebotomy in order to quantitate the availability of iron dextran stores for Hb synthesis. When approximately 70% of the calculated iron dextran store of 1 050 mg had been mobilized, an iron deficient erythropoiesis had developed. In this state characteristic residual iron deposits in bone marrow smears could be demonstrated in all subjects.

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“…Erythroblasts with green-blue particles on Perls stain were defined as sideroblasts. According to the number of siderotic granules, sideroblasts were classified as type 0 (no granules), type 1 (one to three granules), or type 2 (three or more granules), and their percentages were computed (19).…”

Section: Laboratory Testsmentioning

confidence: 99%

Can the Response to Iron Therapy Be Predicted in Anemic Nondialysis Patients with Chronic Kidney Disease?

Sica¹,

Bârsan²,

Stanciu³

et al. 2010

Clinical Journal of the American Society of Nephrology

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Background and objectives: Anemia is iron responsive in 30 to 50% of nondialysis patients with chronic kidney disease (CKD), but the utility of bone marrow iron stores and peripheral iron indices to predict the erythropoietic response is not settled. We investigated the accuracy of peripheral and central iron indices to predict the response to intravenous iron in nondialysis patients with CKD and anemia.Design, setting, participants, & measurements: A diagnostic study was conducted on 100 nondialysis patients who had CKD and anemia and were erythropoiesis-stimulating agent and iron naive. Bone marrow iron stores were evaluated by aspiration. Hemoglobin, transferrin saturation index (TSAT), and ferritin were measured at baseline and 1 month after 1000 mg of intravenous iron sucrose. Posttest predictive values for the erythropoietic response (>1-g/dl increase in hemoglobin) of peripheral and central iron indices were calculated.Results: The erythropoietic response was noted in a higher proportion in bone marrow iron-deplete than in iron-replete patients (63 versus 30%). Peripheral iron indices had a moderate accuracy in predicting response. The positive (PPV) and negative predictive values (NPV) were 76 and 72% for a TSAT of 15% and 74 and 70% for a ferritin of 75 ng/ml, respectively. In the final logistic regression model, including TSAT and ferritin, the chances of a positive response increased by 7% for each 1% decrease in TSAT.Conclusions: Because an erythropoietic response is seen in half of patients and even one third of those with iron-replete stores responded whereas peripheral indices had only a moderate utility in predicting response, the therapeutic trial to intravenous iron seems to be a useful tool in the management of anemia in nondialysis patients with CKD.

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Hemosiderin Estimations and Sideroblast Counts in the Differential Diagnosis of Iron Deficiency and other Anemias

Cited by 72 publications

References 33 publications

Similar cellular migration patterns from niches in intervertebral disc and in knee-joint regions detected by in situ labeling: an experimental study in the New Zealand white rabbit

Similar cellular migration patterns from niches in intervertebral disc and in knee-joint regions detected by in situ labeling: an experimental study in the New Zealand white rabbit

Availability of Iron Dextran for Hemoglobin Synthesis

Can the Response to Iron Therapy Be Predicted in Anemic Nondialysis Patients with Chronic Kidney Disease?

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