Hemosiderin V: The occurrence of heme and lipids in hemosiderin

Ludewig, Stephan; Franz, S

doi:10.1016/0003-9861(70)90362-0

Cited by 11 publications

(6 citation statements)

References 12 publications

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“…Haem and porphyrin compounds were not found (<0.1 nmol/mg of haemosiderin), in agreement with Van Leeuwen et al (1977), who found that haemosiderin only contained haem if the tissue was left unfrozen after death; the occurrence of haem in some preparations (Ludewig & Franz, 1970;McKay & Fineberg, 1964b) may be attributable to this.…”

Section: S3supporting

confidence: 87%

“…Iron and protein contents of haemosiderin prepartions are given in Table 1. The iron content compares closely with the values found for horse spleen (McKay & Fineberg, 1964a) and human liver (Ludewig & Franz, 1970) haemosiderin. Our data indicate iron/protein weight ratios of 1.4-2.0, Table 1.…”

Section: Analysis Of Haemosiderinsupporting

confidence: 83%

“…Galactose (200 pmol/g), mannose (100pmol/g) and a small amount of fucose (20 pmol/g) were detected in haemosiderin. However, hexosamines and sialic acid were not found (<5pumol/g); N-acetylgalactosamine and N-acetylglucosamine are common constituents of mammalian carbohydrate side chains (Kornfeld & Kornfeld, 1976) and their absence here, together with the generally low levels and variability in the carbohydrate compositions reported previously (McKay & Fineberg, 1964a;Ludewig & Franz, 1970), suggests that these sugars are contaminants.…”

Section: S3mentioning

confidence: 44%

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Biochemical studies on the isolation and characterization of human spleen haemosiderin

Weir¹,

Gibson²

1984

Biochemical Journal

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Haemosiderin was isolated from thalassaemic human spleens by centrifugation through concentrated KI solutions. A method for solubilizing haemosiderin was developed which leaves the iron oxyhydroxide cores and constituent polypeptides intact, facilitating further purification and analysis. Purified haemosiderin contained no detectable haem, trace amounts of carbohydrate, and iron and phosphorus in a molar ration of 6:1; much of the phosphate may be present as core-adsorbed. Several lipids were present, but it is not certain whether these are contaminants or components of the haemosiderin granules. In all preparations examined, a characteristic group of six to seven peptides of apparent Mr 12 900-17 800 were found, with a major band at Mr 14 500 and, in addition, a minor component of Mr 42 000; these peptides co-chromatographed with the cores. Negatively stained electron micrographs suggest that these peptides form an incomplete shell about the cores, consistent with the view that haemosiderin is a proteolytic product of ferritin.

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Section: S3supporting

confidence: 87%

Section: Analysis Of Haemosiderinsupporting

confidence: 83%

Section: S3mentioning

confidence: 44%

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Biochemical studies on the isolation and characterization of human spleen haemosiderin

Weir¹,

Gibson²

1984

Biochemical Journal

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“…Hemosiderin Hemosiderin was prepared from pig liver by the method of Ludewig and Franz (1970). It was lyophilized and stored at 4'C.…”

Section: Methodsmentioning

confidence: 99%

Hemoglobin Replection and the Hematological Response of Anemic Rats to Hemosiderin and Ferrous Sulfate.

Kongkachuichai

Yasumoto

1997

FSTI

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“…Iron standard solution (ferric nitrate in 0.275 M nitric acid) was obtained from Nacalai Tesque, Inc. (Kyoto, Japan), hemin and myoglobin from Sigma Chemical Co. (St. Louis, MO). Hemosiderin was extracted from porcine liver by a modified method of Ludewig and Franz (1970). The final preparation of hemosiderin contained on analysis 484 mg iron per gram of protein.…”

Section: Samplesmentioning

confidence: 99%