Hemostasis in the Mouse (Mus musculus): A Review

Tsakiris, Dimitrios Α.; Scudder, Lesley E.; Hodivala‐Dilke, Kairbaan; Hynes, Richard O.; Coller, Barry S.

doi:10.1055/s-0037-1614439

Cited by 118 publications

(122 citation statements)

References 172 publications

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“…Hence, the mouse protocols give reproducible and verifiable results. Most significantly, the PT, APTT, and Fib strain characterization data (see below) are consistent with previously reported results (10).…”

Section: Modification Of the Dade Behring Bcs Protocolssupporting

confidence: 92%

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice*

Peters

Cheever

Ellis

et al. 2002

Physiological Genomics

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The Mouse Phenome Project is an international effort to systematically gather phenotypic data for a defined set of inbred mouse strains. For such large-scale projects the development of high-throughput screening protocols that allow multiple tests to be performed on a single mouse is essential. Here we report hematologic and coagulation data for more than 30 inbred strains. Complete blood counts were performed using an Advia 120 analyzer. For coagulation testing, we successfully adapted the Dade Behring BCS automated coagulation analyzer for use in mice by lowering sample and reagent volume requirements. Seven automated assay procedures were developed. Small sample volume requirements make it possible to perform multiple tests on a single animal without euthanasia, while reductions in reagent volume requirements reduce costs. The data show that considerable variation in many basic hematological and coagulation parameters exists among the inbred strains. These data, freely available on the World Wide Web, allow investigators to knowledgeably select the most appropriate strain(s) to meet their individual study designs and goals.

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Section: Modification Of the Dade Behring Bcs Protocolssupporting

confidence: 92%

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice*

Peters

Cheever

Ellis

et al. 2002

Physiological Genomics

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“…Normal platelet survival was observed in all the GPIbβ -/-mice evaluated in this study, but caution should be exercised when comparing results for mice and men, especially in view of the differences in terms of platelet count and size between the two species. 34 On the other hand, the results in mice and the above previous observation of patients with a normal platelet half-life suggest that in addition to species differences, technical factors could be responsible for the shorter survival measured in some individuals. Giant platelets are difficult to isolate and might be activated during 111 In-labeling 33 and artificially removed from the circulation upon re-injection into patients.…”

Section: Discussionmentioning

confidence: 88%

Intrinsic impaired proplatelet formation and microtubule coil assembly of megakaryocytes in a mouse model of Bernard-Soulier syndrome

Strassel¹,

Eckly²,

Léon³

et al. 2009

Haematologica

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The online version of this article contains a supplementary appendix. BackgroundGiant platelets and thrombocytopenia are invariable defects in the Bernard-Soulier syndrome caused by deficiency of the GPIb-V-IX complex, a receptor for von Willebrand factor supporting platelet adhesion to the damaged arterial wall. Various properties of this receptor may be considered potential determinants of the macrothrombocytopenia. Design and MethodsTo explore the underlying mechanisms of the disease, megakaryopoiesis was studied in a mouse model deficient in GPIbβ. Megakaryocytes were initially characterized in situ in the bone marrow of adult mice, after which their capacity to differentiate into proplateletbearing cells was evaluated in cultured fetal liver cells. ResultsThe number of megakaryocyte progenitors, their differentiation and progressive maturation into distinct classes and their level of endoreplication were normal in GPIbβ -/-bone marrow. However, the more mature cells exhibited ultrastructural anomalies with a thicker peripheral zone and a less well developed demarcation membrane system. GPIbβ -/-megakaryocytes could be differentiated in culture from Lin -fetal liver cells in normal amounts but the proportion of cells able to extend proplatelets was decreased by 41%. Moreover, the GPIbβ -/-cells extending proplatelets displayed an abnormal morphology characterized by fewer pseudopodial extensions with thicker shaft sections and an increased diameter of the terminal coiled elements. GPIbβ -/-released platelets were larger but retained a typical discoid shape. Proplatelet formation was similarly affected in bone marrow explants from adult mice examined by videomicroscopy. The marginal microtubular ring contained twice as many tubulin fibers in GPIbβ -/-proplatelet buds in cultured and circulating platelets. ConclusionsAltogether, these findings point to a role of the GPIb-V-IX complex intrinsic to megakaryocytes at the stage of proplatelet formation and suggest a functional link with the underlying microtubular cytoskeleton in platelet biogenesis.Key words: megakaryocyte, platelet, Bernar-Soulier, microtubules, GPIb-V-IX.Citation: Strassel C, Eckly A, Léon C, Petitjean C, Freund M, Cazenave J-P, Gachet C, and Lanza F. Intrinsic impaired proplatelet formation and microtubule coil assembly of megakaryocytes in a mouse model of Bernard-Soulier syndrome. Haematologica 2009; 94:800-810. doi:10.3324/haematol.2008 This is an open-access paper.

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“…In all animal studies, the environment of blood collection can have a profound effect on platelet function studies, as any stress or anxiety in the animal can alter platelet counts as well as responsiveness (18). Similarly, many drugs used as anesthetics can affect platelet function (19). In these studies, isoflurane was used because we found that it did not affect platelet function, whereas other drugs such as ketamine adversely affected the study measurements.…”

Section: Discussionmentioning

confidence: 94%

“…We found approximately 700,000 platelets /µL in canine blood, in agreement with published data (18). Mice also have high platelet counts of up to 4 times the normal human platelet count (19). This may have the effect of simulating a lower dose of peptide, since so many more receptors are available for binding.…”

Section: Discussionmentioning

confidence: 99%

Platelet binding and biodistribution of [99mTc]rBitistatin in animal species and humans

Knight

Romano

Bright

et al. 2007

Nuclear Medicine and Biology

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INTRODUCTION-99m Tc-rBitistatin is a radioligand for the αIIbβ3 (GPIIb/IIIa) receptor on platelets and is being developed as a diagnostic radiopharmaceutical for in vivo imaging of acute thrombi and emboli. Prior to the first administration of 99m Tc-rBitistatin to human subjects, its biodistribution and effects on platelets were evaluated in animals. This paper reports the findings of the animal studies in comparison with the initial findings in normal human subjects.

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Hemostasis in the Mouse (Mus musculus): A Review

Cited by 118 publications

References 172 publications

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice*

Large-scale, high-throughput screening for coagulation and hematologic phenotypes in mice*

Intrinsic impaired proplatelet formation and microtubule coil assembly of megakaryocytes in a mouse model of Bernard-Soulier syndrome

Platelet binding and biodistribution of [99mTc]rBitistatin in animal species and humans

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