Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

Teramura, Yuji; Okamura, Yosuke; Takeoka, Shinji; Tsuchiyama, Hiromi; Narumi, Hideki; Kainoh, Mie; Handa, Makoto; Ikeda, Yasuo; Tsuchida, Eishun

doi:10.1016/s0006-291x(03)00943-4

Cited by 25 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To prepare the PLT substitutes enhancing the hemostatic ability, we also conjugated fibrinogen 17 to biocompatible carriers such as polymerized albumin particles (polyAlb) 18,19 and phospholipid vesicles (liposomes) 20‐24 . These fibrinogen conjugates were shown to facilitate PLT aggregation on an activated PLT‐immobilized surface in vitro by recruitment of the flowing PLTs in the aggregates after their attachment 17 .…”

mentioning

confidence: 99%

“…14,15 This is because fibrinogen contains three putative binding sites to GPIIb/IIIa, namely, a tetrapeptide containing an RGD sequence, for example, RGDF and RGDS at a95-98 and a572-575, respectively, and a dodecapeptide (HHLGGAKQAGDV, H12) at a a-chain carboxyterminal segment (a400-411). 16 To prepare the PLT substitutes enhancing the hemostatic ability, we also conjugated fibrinogen 17 to biocompatible carriers such as polymerized albumin particles (polyAlb) 18,19 and phospholipid vesicles (liposomes). [20][21][22][23][24] These fibrinogen conjugates were shown to facilitate PLT aggregation on an activated PLT-immobilized surface in vitro by recruitment of the flowing PLTs in the aggregates after their attachment.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prolonged hemostatic ability of polyethylene glycol–modified polymerized albumin particles carrying fibrinogen γ‐chain dodecapeptide

Okamura

Fujie²,

Maruyama³

et al. 2007

Transfusion

Self Cite

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Prolonged hemostatic ability of polyethylene glycol–modified polymerized albumin particles carrying fibrinogen γ‐chain dodecapeptide

Okamura

Fujie²,

Maruyama³

et al. 2007

Transfusion

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some research groups have focused on developing hemostatic materials based on functionalized nanoparticles capable of aggregating platelets from blood flow [108–111]. For example, Ravikumar et al created hemostatic nanomaterials through the surface functionalization of liposomes with 150 nm diameters with three peptides including von Willebrand factor (vWF)-binding peptide (VBP), collagen-binding peptide (CBP) and cyclic-Arg-Gly-Asp (cRGD) peptide [109].…”

Section: Nanoparticle Incorporated Surgical Materialsmentioning

confidence: 99%

Surgical materials: Current challenges and nano-enabled solutions

et al. 2014

View full text Add to dashboard Cite

Surgical adhesive biomaterials have emerged as substitutes to sutures and staples in many clinical applications. Nano-enabled materials containing nanoparticles or having a distinct nanotopography have been utilized for generation of a new class of surgical materials with enhanced functionality. In this review, the state of the art in the development of conventional surgical adhesive biomaterials is critically reviewed and their shortcomings are outlined. Recent advancements in generation of nano-enabled surgical materials with their potential future applications are discussed. This review will open new avenues for the innovative development of the next generation of tissue adhesives, hemostats, and sealants with enhanced functionality for various surgical applications.

show abstract

“…We have developed biocompatible and biodegradable nanoparticles such as albumin-based nanoparticles [2][3][4][5] and phospholipid vesicles 6,7 carrying recombi-nant fragments of platelet membrane proteins 3,4,6,8 and dodecapeptide as a recognition site for fibrinogen. 2,5,7,9 These nanoparticles specifically recognized the site of bleeding injury or activated platelets.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of free‐standing albumin‐nanosheets having heterosurfaces

Okamura

Goto

Niwa

et al. 2008

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

Sheet-shaped carriers, having both obverse and reverse surfaces and thus a large contact area for targeting a site, have several advantages over spherical-shaped carriers, which have an extremely small contact area for targeting sites. Here, we proposed a novel method to prepare a free-standing ultrathin and biocompatible nanosheet having heterosurfaces, by a combination of four processes: (1) specific adsorption of recombinant human serum albumin (rHSA) molecules onto a patterned octadecyltrimethoxysilane self-assembled monolayer region (ODS-SAM), (2) preparation of nanosheets of rHSA molecules bearing thiol groups (SH-rHSA) via two-dimensionally disulfide crosslinking, (3) surface modification of the resulting nanosheet, and (4) preparation of the free-standing nanosheet by detachment from the ODS-SAM. The SH-rHSA molecules at pH 5.0 and a concentration of 1 microg/mL were specifically adsorbed on the patterned ODS-SAM regions by hydrophobic interaction, and were two-dimensionally crosslinked in the presence of copper ion as an oxidant. The rHSA-nanosheets were then simply detached from the ODS-SAM by treatment with surfactant. We succeeded in the preparation of rectangular (10 microm x 30 microm) and ultrathin (4.5 +/- 1.0 nm) rHSA-nanosheets on a patterned ODS-SAM, and could also obtain free-standing rHSA-nanosheets having heterosurfaces by surface modification with fluorescent latex beads. Thus, the rHSA-nanosheets having heterosurfaces could be regarded as a new biomaterial for drug carriers, hemostatic reagents, wound dressing for burn injury, and so forth.

show abstract

Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice

Cited by 25 publications

References 26 publications

Prolonged hemostatic ability of polyethylene glycol–modified polymerized albumin particles carrying fibrinogen γ‐chain dodecapeptide

Prolonged hemostatic ability of polyethylene glycol–modified polymerized albumin particles carrying fibrinogen γ‐chain dodecapeptide

Surgical materials: Current challenges and nano-enabled solutions

Fabrication of free‐standing albumin‐nanosheets having heterosurfaces

Contact Info

Product

Resources

About