Hempseed (Cannabis sativa) protein hydrolysates: A valuable source of bioactive peptides with pleiotropic health-promoting effects

Santos-Sánchez, Guillermo; Álvarez-López, Ana Isabel; Ponce-España, Eduardo; Carrillo-Vico, Antonio; Bollati, Carlotta; Bartolomei, Martina; Lammi, Carmen; Cruz‐Chamorro, Ivan

doi:10.1016/j.tifs.2022.06.005

Cited by 27 publications

(13 citation statements)

References 85 publications

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“…Food-derived peptides have gained the attention of the scientific community because of their several beneficial health effects [2,17]. These pleiotropic effects have been demonstrated to be linked mainly to the physicochemical composition of the peptides, which in turn, is related to the conditions in which the hydrolysis process occurs [13,35,36].…”

Section: Discussionmentioning

confidence: 99%

“…Although food-derived bioactive peptides have shown numerous positive health effects, some factors limit their commercialization, including their bitter taste, hygroscopicity, and low bioavailability [17,42]. As is well known, after ingestion, the structure and therefore the bioactivity of peptides can change during the processes of digestion, absorption, and transport through the intestinal epithelial cells toward the blood circulatory system.…”

Section: Discussionmentioning

confidence: 99%

“…It contains approximately 24% (w/w) protein, and edestin accounts for approximately 60-80% (w/w) of its total proteins [14][15][16]. Hempseed proteins have been hydrolyzed into peptides with different properties [17], including antihypertensive [18][19][20], α-glucosidase inhibitory [21], hypocholesterolemic [22,23], anti-inflammatory [24], and antioxidant [25,26]. The present study, considering the current interest in antioxidant peptides obtained from food sources, aims to promote the bioactivities of two hempseed protein hydrolysates (HHs) by focusing the interest on the identification and characterization of bioavailable antioxidant peptides.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates

Santos-Sánchez

Aiello²,

Rivardo³

et al. 2023

Antioxidants

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Hempseed (Cannabis sativa) is one of the most promising sources of plant proteins. It contains approximately 24% (w/w) protein, and edestin accounts for approximately 60–80% (w/w) of its total proteins. In a framework of research aimed at fostering the proteins recovered from the press cake by-products generated after the extraction of hempseed oil, two hempseed protein hydrolysates (HH1 and HH2) were produced at an industrial level using a mixture of different enzymes from Aspergillus niger, Aspergillus oryzae, and Bacillus licheniformis for different times (5 h and 18 h). Using a combination of different direct antioxidant tests (DPPH, TEAC, FRAP, and ORAC assays, respectively), it has been demonstrated that HHs exert potent, direct antioxidant activity. A crucial feature of bioactive peptides is their intestinal bioavailability; for this reason, in order to solve this peculiar issue, the ability of HH peptides to be transported by differentiated human intestinal Caco-2 cells has been evaluated. Notably, by using mass spectrometry analysis (HPLC Chip ESI-MS/MS), the stable peptides transported by intestinal cells have been identified, and dedicated experiments confirmed that the trans-epithelial transported HH peptide mixtures retain their antioxidant activity, suggesting that these hempseed hydrolysates may be considered sustainable antioxidant ingredients to be exploited for further application, i.e., nutraceutical and/or food industries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates

Santos-Sánchez

Aiello²,

Rivardo³

et al. 2023

Antioxidants

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“…The ORAC assay, based on the scavenging of peroxyl radicals generated by AAPH, was performed following the Santos-Sánchez et al method [ 27 ]. Briefly, 25 µL of FPH (at the final concentrations of 0.1, 0.5 and 1.0 mg/mL) was added to 50 µL sodium fluorescein (2.934 mg/L) and incubated for 15 min at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Rainbow Trout (Oncorhynchus mykiss) as Source of Multifunctional Peptides with Antioxidant, ACE and DPP-IV Inhibitory Activities

et al. 2023

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The present study aimed at characterizing the possible biological activities of the multifunctional low molecular weight fractions (<3 kDa) peptides isolated from rainbow trout (Oncorhynchus mykiss) obtained by enzymatic hydrolysis. The fish protein hydrolysate (FPH) was tested for its antioxidant property along with its angiotensin converting enzyme (ACE) and dipeptidyl peptidase IV (DPP-IV) inhibitory activities. In particular, the 2,2-diphenyl-1-picrylhydrazyl (DPPH), the ferric reducing antioxidant power (FRAP), the oxygen radical absorbance capacity (ORAC) assay and the 2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays were carried out for the evaluation of the in vitro antioxidant activity. The cell-free ACE and DPP-IV inhibitory activity assays were also estimated, showing a dose-dependent inhibition. These biological properties were additionally quantified at the cellular level using human intestinal Caco-2 cells. Namely, the antioxidant activity was determined by evaluating the capability of the hydrolysate to reduce the H2O2-induced reactive oxygen species (ROS) and lipid peroxidation levels, and the DPP-IV activity assays show a reduction of enzyme activity of up to 27.57 ± 3.7% at 5 mg/mL. The results indicate that Oncorhynchus mykiss-derived peptides may have potential employment as health-promoting ingredients.

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“…Studies have shown that imbalances in the RAAS system pathway foster hypertension progression. [ 41 ] Specifically, a drop in blood pressure triggers the cascade of enzymatic reactions in the RAAS system in a homeostatic state. First, the renin precursor protein (prorenin), which is abundant in juxtaglomerular cells of the kidney, is cleaved to renin and secreted into the blood.…”

Section: Biological Targets Of Microalgae Peptides For Promoting Card...mentioning

confidence: 99%

Big Things, Small Packages: An Update on Microalgae as Sustainable Sources of Nutraceutical Peptides for Promoting Cardiovascular Health

et al. 2023

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In 2017, a review of microalgae protein‐derived bioactive peptides relevant in cardiovascular disease (CVD) management was published. Given the rapid evolution of the field, an update is needed to illumininate recent developments and proffer future suggestions. In this review, the scientific literature (2018–2022) is mined for that purpose and the relevant properties of the identified peptides related to CVD are discussed. The challenges and prospects for microalgae peptides are similarly discussed. Since 2018, several publications have independently confirmed the potential to produce microalgae protein‐derived nutraceutical peptides. Peptides that reduce hypertension (by inhibiting angiotensin converting enzyme and endothelial nitric oxide synthase), modulate dyslipidemia and have antioxidant and anti‐inflammatory properties have been reported, and characterized. Taken together, future research and development investments in nutraceutical peptides from microalgae proteins need to focus on the challenges of large‐scale biomass production, improvement in techniques for protein extraction, peptide release and processing, and the need for clinical trials to validate the claimed health benefits as well as formulation of various consumer products with the novel bioactive ingredients.

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Hempseed (Cannabis sativa) protein hydrolysates: A valuable source of bioactive peptides with pleiotropic health-promoting effects

Cited by 27 publications

References 85 publications

Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates

Antioxidant Effect Assessment and Trans Epithelial Analysis of New Hempseed Protein Hydrolysates

Rainbow Trout (Oncorhynchus mykiss) as Source of Multifunctional Peptides with Antioxidant, ACE and DPP-IV Inhibitory Activities

Big Things, Small Packages: An Update on Microalgae as Sustainable Sources of Nutraceutical Peptides for Promoting Cardiovascular Health

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