Heparan sulfate mimetics modulate calpain activity during rat Soleus muscle regeneration

Zimowska, Małgorzata; Szczepankowska, Dorota; Stremińska, Władysława; Papy, Dulce; Tournaire, Marie-Claude; Gautron, J; Barritault, Denis; Moraczewski, Jerzy; Martelly, Isabelle

doi:10.1002/jcp.1106

Cited by 21 publications

(19 citation statements)

References 64 publications

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“…In crush induced regeneration model, EDL muscle regenerates properly and shows a normal structure at day 14 after wounding (Bassaglia and Gautron, 1995). In contrast, Soleus muscle undergoes fibrosis after crush and denervation (Bassaglia and Gautron, 1995, Moraczewski et al, 1996, Zimowska et al, 2001.…”

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confidence: 99%

Distinct patterns of MMP-9 and MMP-2 activity in slow and fast twitch skeletal muscle regeneration in vivo

Zimowska¹,

Brzóska²,

Swierczynska³

et al. 2008

Int. J. Dev. Biol.

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Skeletal muscles exhibit great plasticity and an ability to reconstruct in response to injury. However, the repair process is often inefficient and hindered by the development of fibrosis. We explored the possibility that during muscle repair, the different regeneration ability of the fast (extensor digitorum longus; EDL) and slow twitch (Soleus) muscles depends on the differential expression of metalloproteinases (MMP-9 and MMP-2) involved in the remodeling of the extracellular matrix. Our results show that MMP-9 and MMP-2 are present in the intact muscle and are up-regulated after crush-induced muscle injury. The expression and the activity of these two enzymes depend on the type of muscle and the phase of muscle regeneration. In the regenerating Soleus muscle, elevated levels of MMP-9 occurred during the myolysis and reconstruction phase. In contrast, regenerating EDL muscles exhibited decreased MMP-9 levels during myolysis and increased MMP-2 activity at the reconstruction phase. Moreover, satellite cells (mononuclear myoblasts) derived from Soleus and EDL muscles showed no differences in localization or activity of MMP-9 and MMP-2 during proliferation and differentiation in vitro. MMP-9 activity was present during all stages of myoblast differentiation, whereas MMP-2 activity reached its highest level during myoblast fusion. We conclude that MMPs are involved in muscle repair, and that fast and slow twitch muscles exhibit different patterns of MMP-9 and MMP-2 activity.

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confidence: 99%

Distinct patterns of MMP-9 and MMP-2 activity in slow and fast twitch skeletal muscle regeneration in vivo

Zimowska¹,

Brzóska²,

Swierczynska³

et al. 2008

Int. J. Dev. Biol.

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“…Since, stabilized reinnervation and reduced fibrosis was observed in regenerating soleus muscle treated with RGTA, thus we decided to investigate the changes in expression of µ-and m-calpain during Soleus muscle regeneration and to explore whether RGTA treatment would influence calpain activity in these muscles (Zimowska et al, 2001). Microand milli-calpain levels increased during the fiber reconstruction and reinnervation.…”

Section: Calpain Activitymentioning

confidence: 99%

“…The extent of degeneration of the extracellular matrix may be a limiting factor in regeneration. In our studies, we elucidated crucial differences in the regeneration of fast-and slow-contracting muscle, as well as the participation of calpains in various stages of myolysis and reconstruction (Moraczewski et al, 1996a, Moraczewski et al, 1996b, Zimowska et al, 2001. …”

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confidence: 95%

From Planarians to Mammals - the many faces of regeneration

Moraczewski

Archacka²,

Brzóska³

et al. 2008

Int. J. Dev. Biol.

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This report presents the history of the involvement of the Department of Cytology in studies of different aspects of regeneration. It can be divided into two major phases; the first focused on the regeneration of Turbellarians and the second on the regeneration of rat skeletal muscles including the differentiation of satellite cells in vitro. Regeneration of Turbellarians was investigated both at the cellular and molecular levels including the role of the protein kinase C (PKC) in this process. Studies on skeletal muscle regeneration initially focused on factors involved in regulation of signal transduction pathways. Next, we explored the influence of growth factors on the modulation of the regeneration process. Another important aspect of our studies was investigating of the distribution and function of different proteins involved in adhesion and fusion of myoblasts. Finally, we are also conducting research on the role of stem cells from other tissues in the regeneration of skeletal muscle. KEY WORDS: regeneration, planaria, skeletal muscleIn this report we present the history of the involvement of the Department of Cytology team in the studies on regeneration dated from 1974. This research may be divided into two major phases. First, we concentrated our efforts on the regeneration of Turbellarians. Then, in 1987, we switched our research model to the regeneration of the rat skeletal muscle and the differentiation of satellite cells in vitro. In this article we demonstrate the main topics of the studies conducted solely by the members of our group or in collaboration with other laboratories. Planarian regenerationPlanarians serve as a good model for the study of cell differentiation. Due the presence of totipotent stem cells they are characterized by an unique ability to regenerate. These stem cells (neoblasts) are responsible for the regeneration and growth. Neoblasts are true totipotent stem cells, like embryonic stem cells in vertebrates. They can be involved in the differentiation of all planarian tissues. Turbellarian of the order Catenulida were cultured in our laboratory for 6 years and served those days as a subject of various studies (Fig. 1). Our first publication in that we focused at Catenula concerned its asexual reproduction and regeneration (Moraczewski, 1977). The examination of the ultrastructure of the myodermal body wall by (Moraczewski and Czubaj, 1974) and (Soltynska et al., 1976), as well as of the nervous system by (Moraczewski et al., 1977a, Moraczewski et Int. J. Dev. Biol. 52: 219-227 (2008) ., 1977b), made it possible to trace precisely the course of regeneration and paratomy i.e. transverse fission during which differentiation of new organs occurred prior to separation from the mother animal ( Fig. 1 A,B). These studies showed that fissioning in Catenula was a typical paratomy as defined by (Wagner, 1890). The Catenula species analyzed by us always divided into only two zooids, and fission always occurred behind the intestine. The opisthe i.e. he posterior daughter org...

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“…Recently, it has been shown that glycosaminoglycan mimetics, synthetic derivatives of dextran, stimulate myogenesis while changing the natural glycosaminoglycan composition, notably heparan sulphate [42]. These glycosaminoglycan mimetics stimulate the regeneration of denervated and crushed skeletal muscles [43,44], as well as prevent most of the damage resulting from acute skeletal or cardiac muscle ischaemia [45]. Therefore, these dextran polymers were called regenerating agents (RGTA).…”

Section: Increased Expression Of a Chondroitin Sulphate Epitopementioning

confidence: 99%

Diaphragm dysfunction in chronic obstructive pulmonary disease: a role for heparan sulphate?

Ottenheijm¹,

Jenniskens²,

Geraedts³

et al. 2007

Eur Respir J

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In the present study, phage display-derived antibodies were used to investigate the topology of glycosaminoglycan epitopes in the diaphragm of chronic obstructive pulmonary disease (COPD) and non-COPD patients. Furthermore, the potential physiological significance of changes in the occurrence of glycosaminoglycan epitopes in the diaphragm of COPD patients was studied by determining the overlap in epitope recognition of glycosaminoglycan antibodies and growth factors.Diaphragm cryosections from non-COPD (n55) and COPD patients (Global Initiative for Chronic Obstructive Lung Disease (GOLD) stage I/II; n59) were incubated with antibodies directed against heparan sulphate, chondroitin sulphate and dermatan sulphate epitopes. Antibodies were visualised immunofluorescently. In addition, interference of antibody and growth factor binding with heparan sulphate epitopes was tested.Specific glycosaminoglycan epitopes showed increased expression in the diaphragm of COPD patients, whereas other epitopes were decreased or unaffected. Interestingly, the anti-heparan sulphate antibody HS4C3, which is directed against a downregulated epitope, interfered with the binding of hepatocyte growth factor. Three patients with the most severe airway obstruction also demonstrated interference of heparan sulphate antibody A04B08 with hepatocyte growth factor binding.Results indicate changes in glycosaminoglycan composition in the diaphragm of patients with chronic obstructive pulmonary disease. This may affect cellular physiology via alterations in growth factor handling and might be related to reduced levels of contractile protein in the diaphragm of these patients.

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Heparan sulfate mimetics modulate calpain activity during rat Soleus muscle regeneration

Cited by 21 publications

References 64 publications

Distinct patterns of MMP-9 and MMP-2 activity in slow and fast twitch skeletal muscle regeneration in vivo

Distinct patterns of MMP-9 and MMP-2 activity in slow and fast twitch skeletal muscle regeneration in vivo

From Planarians to Mammals - the many faces of regeneration

Diaphragm dysfunction in chronic obstructive pulmonary disease: a role for heparan sulphate?

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