Heparin-coated oxygenators significantly reduce contact system activation in an in vitro cardiopulmonary bypass model

Wendel, Hans-Peter; Heller, W.; Gallimore, Michael J.; Hoffmeister, H. E.

doi:10.1097/00001721-199410000-00001

Cited by 27 publications

(16 citation statements)

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“…Excellent resuscitation was obtained (data not shown), and no blood coagulation or microclots were found in the coated devices. Numerous studies [27][28][29] in which the same type of coating in cardiopulmonary bypass was used have shown greatly improved outcome. Release of heparin from the coating was virtually undetectable.…”

Section: Discussionmentioning

confidence: 99%

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

Liachenko

Tang

Hamilton

et al. 1998

Stroke

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Background and Purpose-Because noninvasive physiological monitoring of cerebral blood flow, metabolic integrity, and brain ion and water homeostasis can now be accomplished with new, state-of-the-art MR spectroscopy and imaging techniques, it is appropriate to develop controllable and reproducible animal models that permit prolonged circulatory arrest and resuscitation in the magnet and also allow for studies of long-term survival and outcome. We have developed such a model in rats that involves minimal surgical preparations and can achieve resuscitation remotely within precisely controlled time. Methods-Cardiac arrest was induced by asphyxiation, the duration of which ranged from 8 to 24 minutes. Resuscitation was achieved remotely by a slow, intra-aortic infusion of oxygenated blood (withdrawn either from the same rat before asphyxia or from a healthy donor rat) along with a resuscitation cocktail containing heparin (50 U/100 g), sodium bicarbonate (0.1 mEq/100 g), and epinephrine (4 g/100 g). The body temperature was measured by a tympanic thermocouple probe and was controlled either by a heating pad (constant tympanic temperatureϭ37°C) or by warm ambient air (constant air temperatureϭ37°C). Interleaved 31 P/ 1 H nuclear magnetic resonance (NMR) spectroscopy was used in a selected group of rats to measure the cerebral metabolism before and during approximately 20 minutes of circulatory arrest and after resuscitation. Results-The overall success rate of resuscitation, irrespective of the duration of cardiac arrest, was 82% (51 of 62). With a programmed infusion pump, the success rate was even higher (95%). The survival time for rats subjected to 15 and 19 minutes of asphyxia with core temperature tightly controlled was significantly lower than that with ambient temperature control (PϽ0.001 and PϽ0.04, respectively). High-quality NMR spectra can be obtained continuously without interference from the resuscitation effort. Final histological examinations taken 5 days after resuscitation showed typical neuronal damages, similar to those found in other global ischemia models. Conclusions-Because the no-flow time and resuscitation time can be precisely controlled, this outcome model is ideally suited for studies of ischemic and reperfusion injuries in the brain and possibly in other critical organs, permitting continuous assessment of long-term recovery and follow-up in the same animals. (Stroke. 1998;29:1229-1239.)

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Section: Discussionmentioning

confidence: 99%

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

Liachenko

Tang

Hamilton

et al. 1998

Stroke

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“…Heparin-bonded extracorporeal circuits may improve biocompatibility in terms of coagulation system activation, as has been established in dialysis [23] and cardiopulmonary bypass [24], though no such circuit is in general clinical use for haemofiltration. Whether the results of the dialysis or cardiopulmonary bypass biocompatibility studies can be extrapolated to haemofiltration is questionable, especially as the patient population and length of patient exposure to the filter are markedly different.…”

Section: Discussionmentioning

confidence: 99%

Continuous venovenous haemofiltration using polyacrylonitrile filters does not activate contact system and intrinsic coagulation pathways

Salmon

Cardigan

Mackie

et al. 1997

Intensive Care Medicine

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The contact system was not activated further by continuous venovenous haemofiltration using polyacrylonitrile filters in critically ill patients. Premature clotting of the haemofilter circuit was more common in patients with very low levels of antithrombin III and heparin co-factor II; although this was related to thrombin generation, the intrinsic coagulation pathway does not appear to be implicated.

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“…The experiments were carried out using a well established in vitro heart-lung-machine model which has been described previously [12]. Fresh human whole blood (not older than 30 min) from healthy volunteers was recirculated in a closedloop CPB system with oxygenation and simulation of arterial counter-pressure.…”

Section: Cardiopulmonary Bypass Modelmentioning

confidence: 99%

The GP IIb/IIIa inhibitor abciximab (ReoPro) decreases activation and interaction of platelets and leukocytes during in vitro cardiopulmonary bypass simulation

Straub

Wendel

Azevedo

et al. 2005

European Journal of Cardio-Thoracic Surgery

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Heparin-coated oxygenators significantly reduce contact system activation in an in vitro cardiopulmonary bypass model

Cited by 27 publications

References 0 publications

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

A Reproducible Model of Circulatory Arrest and Remote Resuscitation in Rats for NMR Investigation

Continuous venovenous haemofiltration using polyacrylonitrile filters does not activate contact system and intrinsic coagulation pathways

The GP IIb/IIIa inhibitor abciximab (ReoPro) decreases activation and interaction of platelets and leukocytes during in vitro cardiopulmonary bypass simulation

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