Heparin-immobilized microspheres for the capture of cytokines

Duo, Jia; Stenken, Julie A.

doi:10.1007/s00216-010-4170-1

Cited by 14 publications

(24 citation statements)

References 28 publications

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“…RANTES and its derivatives are known to have affinity interactions with various glycosaminoglycans(GAGs), with heparin having the strongest affinity, followed by various chondroitin sulfates 27 and uncleaved heparan sulfates 28–31 . These interactions have been explored in the application of biosensor and diagnostic development for cytokines 32, 33 . In this study, we characterized the affinity strength of different GAGs to 5P12-RANTES.…”

Section: Introductionmentioning

confidence: 99%

Using Glycosaminoglycan/Chemokine Interactions for the Long-Term Delivery of 5P12-RANTES in HIV Prevention

et al. 2013

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5P12-RANTES is a recently developed chemokine analog that has shown high level protection from SHIV infection in macaques. However, the feasibility of using 5P12-RANTES as a long term HIV prevention agent has not been explored partially due to the lack of available delivery devices that can easily be modified for long-term release profiles. Glycosaminoglycans (GAGs) have been known for their affinity for various cytokines and chemokines, including native RANTES, or CCL5. In this work, we investigated used of GAGs in generating a chemokine drug delivery device. Initial studies used surface plasmon resonance analysis to characterize and compare the affinities of different GAGs to 5P12-RANTES. These different GAGs were then incorporated into drug delivery polymeric hydrogels to engineer sustained release of the chemokines. In vitro release studies of 5P12-RANTES from the resulting polymers were performed and we found that 5P12-RANTES release from these polymers can be controlled by the amount and type of GAG incorporated. Polymer disks containing GAGs with stronger affinity to 5P12-RANTES resulted in more sustained, and longer term release than did polymer disks containing GAGs with weaker 5P12-RANTES affinity. Similar trends were observed by varying the amount of GAGs incorporated into the delivery system. 5P12-RANTES released from these polymers demonstrated good levels of CCR5 blocking, retaining activity even after 30 days of incubation.

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Section: Introductionmentioning

confidence: 99%

Using Glycosaminoglycan/Chemokine Interactions for the Long-Term Delivery of 5P12-RANTES in HIV Prevention

et al. 2013

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“…The preparation of heparin-immobilized microspheres has been described [31]. A total of 1.0×10 7 heparin-immobilized microspheres were incubated with 500 μL of CCL2 or CCL5 (2.5 ng/mL) in PBS (pH 7.4) containing 0.05% ( w/v ) BSA at room temperature for 2 h. After centrifugation, the supernatant was removed and the cytokine content in the supernatant was determined using the corresponding rat CCL2 or rat CCL5 ELISA kit.…”

Section: Methodsmentioning

confidence: 99%

“…aFGF and VEGF The concentration of aFGF or VEGF in the dialysate collected from the control probe was measured by mixing 15 μL of dialysate with 15 μL of heparin-immobilized microspheres (4.48×10 7 beads/mL) followed by flow cytometric analysis as per the procedure described in the previous paper [31]. Dialysates (30 μL) containing microspheres were analyzed directly by flow cytometry.…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo affinity microdialysis sampling of cytokines using heparin-immobilized microspheres

Duo

Stenken

2010

Anal Bioanal Chem

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Heparin-immobilized microspheres were included in microdialysis sampling perfusion fluids under both in vitro and in vivo conditions to improve the recovery of different cytokines, acidic fibroblast growth factor, vascular endothelial growth factor, monocyte chemoattractant protein-1 (or CCL2), and regulation upon activation normal T cell express sequence (or CCL5). Different strategies to dissociate captured CCL2 and CCL5 from the immobilized heparin were attempted, and both cytokines could be quantitatively eluted from the beads using a phosphate buffer (pH 7.4) containing 25% (v/v) acetonitrile which did not interfere with the subsequent detection of cytokine using an ELISA assay. Using these heparin-immobilized microspheres, a two to fivefold increase of microdialysis relative recovery (RR) was achieved for the four cytokines from a quiescent solution. Enhanced microdialysis RR of CCL2 using the heparin-immobilized microspheres from microdialysis probes implanted into the peritoneal cavity of a rat was performed to test the in vivo application. This work suggests that the heparin-immobilized microspheres provide an alternative affinity agent to the previously used antibody-immobilized microspheres for enhanced microdialysis sampling of cytokines.

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“…Many peptides, including both neuropeptides and other signaling molecules like cytokines, are present at low concentrations making their collection and assay difficult. An improvement in sampling is to add antibodies or other affinity agents to the perfusion flow to enhance the concentration gradient and recovery of the probes (see Figure 1) [21–25]. This approach has been used to enhance recovery several fold for neuropeptides and cytokines.…”

Section: Improvements In Sampling: Large Moleculesmentioning

confidence: 99%

Emerging trends in in vivo neurochemical monitoring by microdialysis

Kennedy

2013

Current Opinion in Chemical Biology

103

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Mapping chemical dynamics in the brain of live subjects is a challenging but highly rewarding goal because it allows neurotransmitter fluctuations to be related to behavior, drug effects, and disease states. A popular method for such measurements is microdialysis sampling coupled to analytical measurements. This method has become well-established for monitoring low molecular weight neurotransmitters, metabolites, and drugs, especially in pharmacological and pharmacokinetic studies. Recent technological developments which improve the temporal and spatial resolution of the methods will enable it to be used for studying behavior and small brain nuclei. Better assays allow monitoring more neurotransmitters simultaneously. Extension to analysis of aggregating proteins like amyloid β are proving extremely useful for uncovering the roles of these molecules and how they contribute to neurodegenerative diseases.

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Heparin-immobilized microspheres for the capture of cytokines

Cited by 14 publications

References 28 publications

Using Glycosaminoglycan/Chemokine Interactions for the Long-Term Delivery of 5P12-RANTES in HIV Prevention

Using Glycosaminoglycan/Chemokine Interactions for the Long-Term Delivery of 5P12-RANTES in HIV Prevention

In vitro and in vivo affinity microdialysis sampling of cytokines using heparin-immobilized microspheres

Emerging trends in in vivo neurochemical monitoring by microdialysis

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