Heparin-Mimicking Multilayer Coating on Polymeric Membrane via LbL Assembly of Cyclodextrin-Based Supramolecules

Deng, Jie; Liu, Xinyue; Ma, Lang; Cheng, Chong; Shi, Wenbin; Nie, Chuanxiong; Zhao, Changsheng

doi:10.1021/am506249r

Cited by 76 publications

(43 citation statements)

References 50 publications

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“…Platelet factor 4 (PF4) was used to detect platelet activation by modified biomedical materials17. Figure 6C shows the PF4 concentrations in plasma after the scaffolds contacted with blood; it could be observed that for the heparinized DLSs the PF4 concentration showed a prominent decrease compared with normal plasma or control DLS.…”

Section: Resultsmentioning

confidence: 99%

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Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization

Bao

Sun

et al. 2015

Sci Rep

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Whole-liver perfusion-decellularization is an attractive scaffold–preparation technique for producing clinical transplantable liver tissue. However, the scaffold’s poor hemocompatibility poses a major obstacle. This study was intended to improve the hemocompatibility of perfusion-decellularized porcine liver scaffold via immobilization of heparin. Heparin was immobilized on decellularized liver scaffolds (DLSs) by electrostatic binding using a layer-by-layer self-assembly technique (/h-LBL scaffold), covalent binding via multi-point attachment (/h-MPA scaffold), or end-point attachment (/h-EPA scaffold). The effect of heparinization on anticoagulant ability and cytocompatibility were investigated. The result of heparin content and release tests revealed EPA technique performed higher efficiency of heparin immobilization than other two methods. Then, systematic in vitro investigation of prothrombin time (PT), thrombin time (TT), activated partial thromboplastin time (APTT), platelet adhesion and human platelet factor 4 (PF4, indicates platelet activation) confirmed the heparinized scaffolds, especially the /h-EPA counterparts, exhibited ultralow blood component activations and excellent hemocompatibility. Furthermore, heparin treatments prevented thrombosis successfully in DLSs with blood perfusion after implanted in vivo. Meanwhile, after heparin processes, both primary hepatocyte and endothelial cell viability were also well-maintained, which indicated that heparin treatments with improved biocompatibility might extend to various hemoperfusable whole-organ scaffolds’ preparation.

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Section: Resultsmentioning

confidence: 99%

“…PF4 has been reported to play a vital role in platelet aggregation24, then, PF4 was used to detect platelet activation by biomedical materials17. Platelet adhesion on the scaffolds is of primary importance for blood compatibility.…”

Section: Discussionmentioning

confidence: 99%

Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization

Bao

Sun

et al. 2015

Sci Rep

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“…In such systems, cyclodextrins (CDs) and cyclodextrin polymers (polyCDs) can be especially used to embed hydrophobic drugs with preserved activity. PEM integrating CDs were presented in the literature for gene delivery, drug delivery, in biomaterials applications …”

Section: Introductionmentioning

confidence: 99%

“…PEM integrating CDs were presented in the literature for gene delivery, 14,15 drug delivery, [16][17][18][19][20] in biomaterials applications. [21][22][23][24][25] The effect of miscellaneous assembly parameters such as solution parameters (e.g. ionic strength, pH, temperature, polyelectrolyte concentration), or intrinsic characteristics of polyelectrolytes (molecular weight, nature, and charge density), and finally the technique used for PEM film deposition play an important role on chemical and physical properties of multilayer self-assemblies, for example film growth, thickness, film hydrophobicity, film hydration and swellability, film stability, surface morphology, and mechanical characteristics.…”

Section: Introductionmentioning

confidence: 99%

Layer‐by‐layer coating of textile with two oppositely charged cyclodextrin polyelectrolytes for extended drug delivery

Junthip

Tabary

Chai

et al. 2016

J Biomedical Materials Res

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The coating of a nonwoven textile by polyelectrolyte multilayer film (PEM) issued from cationic and anionic β-cyclodextrin (βCD) polyelectrolytes according to the layer-by-layer (LbL) technique was successfully attempted. The tert-butyl benzoic acid (TBBA) was used as drug model to evaluate the loading capacity and sustained release properties of this PEM system. The build-up of the multilayer assembly was monitored in situ by optical waveguide lightmode spectroscopy (OWLS) on the one hand, and was assessed by gravimetry on the other hand when applied onto the textile substrate. In parallel, the complexation study of TBBA with both CD polyelectrolytes was also investigated by nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC). The influence of thermal crosslinking of the multilayered coating on its stability and on TBBA release kinetics in phosphate buffered saline (PBS) at 37°C was studied. Finally, biological and microbiological tests were performed to investigate the cytocompatibility and the intrisic antibacterial activity of multilayer assemblies. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1408-1424, 2016.

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“…Thus, it is still a tremendous challenge to comprehensively understand the membrane-associated pathological mechanisms of these amyloidogenic diseases. Artificial biointerfaces, which mimic the membranes from the given surface properties, have been regarded as an effective complement for such research, due to their superior flexibility, tenability, and controllability [45,46]. This review presents various artificial biointerfaces, which are divided into four groups according to their special physical, chemical, or biochemical properties (Figure 1c).…”

Section: Introductionmentioning

confidence: 99%

Protein/Peptide Aggregation and Amyloidosis on Biointerfaces

et al. 2016

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Recently, studies of protein/peptide aggregation, particularly the amyloidosis, have attracted considerable attention in discussions of the pathological mechanisms of most neurodegenerative diseases. The protein/peptide aggregation processes often occur at the membrane–cytochylema interface in vivo and behave differently from those occurring in bulk solution, which raises great interest to investigate how the interfacial properties of artificial biomaterials impact on protein aggregation. From the perspective of bionics, current progress in this field has been obtained mainly from four aspects: (1) hydrophobic–hydrophilic interfaces; (2) charged surface; (3) chiral surface; and (4) biomolecule-related interfaces. The specific physical and chemical environment provided by these interfaces is reported to strongly affect the adsorption of proteins, transition of protein conformation, and diffusion of proteins on the biointerface, all of which are ultimately related to protein assembly. Meanwhile, these compelling results of in vitro experiments can greatly promote the development of early diagnostics and therapeutics for the relevant neurodegenerative diseases. This paper presents a brief review of these appealing studies, and particular interests are placed on weak interactions (i.e., hydrogen bonding and stereoselective interactions) that are also non-negligible in driving amyloid aggregation at the interfaces. Moreover, this paper also proposes the future perspectives, including the great opportunities and challenges in this field as well.

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Heparin-Mimicking Multilayer Coating on Polymeric Membrane via LbL Assembly of Cyclodextrin-Based Supramolecules

Cited by 76 publications

References 50 publications

Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization

Hemocompatibility improvement of perfusion-decellularized clinical-scale liver scaffold through heparin immobilization

Layer‐by‐layer coating of textile with two oppositely charged cyclodextrin polyelectrolytes for extended drug delivery

Protein/Peptide Aggregation and Amyloidosis on Biointerfaces

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