Heparin-modified polylactide as biodegradable hemocompatible biomaterial

Herrmann, K. H.; Groth, Thomas; Seifert, B.; Romaniuk, P

doi:10.1007/bf00120365

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…Other laboratories have applied other components of the endothelial glycocalyx to surfaces to improve the material's biocompatibility and hemocompatibility. For example, Stile et al have grafted hyaluronic acid to glass substrates; and others have improved biocompatibility by employing heparin to coat the surfaces [11-14]. Interestingly, the adsorption of antithrombin among the plasma proteins can be increased by immobilizing antithrombinheparin complex to the surface of a catheter [15].…”

Section: Introductionmentioning

confidence: 99%

Human plasma protein adsorption onto dextranized surfaces: A two-dimensional electrophoresis and mass spectrometry study

Tsai

Tomczyk

Eckmann

et al. 2011

Colloids and Surfaces B: Biointerfaces

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Protein adsorption is fundamental to thrombosis and to the design of biocompatible materials. We report a two-dimensional electrophoresis and mass spectrometry study to characterize multiple human plasma proteins adsorbed onto four different types of model surfaces: silicon oxide, dextranized silicon, polyurethane and dextranized polyurethane. Dextran was grafted onto the surfaces of silicon and polyurethane to mimic the blood-contacting endothelial cell glycocalyx surface. Surface topography and hydrophobicity/hydrophilicity were determined and analyzed using atomic force microscopy and water contact angle measurements, respectively. Using twodimensional electrophoresis, we show that, relative to the unmodified surfaces, dextranization significantly inhibits the adsorption of several human plasma proteins including IGHG1 protein, fibrinogen, haptoglobin, Apo A-IV, Apo A-I, immunoglobulin, serum retinal-binding protein and truncated serum albumin. We further demonstrate the selectivity of plasma protein adsorbed onto the different functionalized surfaces and the potential to control and manipulate proteins adsorption on the surfaces of medical devices, implants and microfluidic devices. This result shows that adsorption experiments using a single protein or a binary mixture of proteins are consistent with competitive protein adsorption studies. In summary, these studies indicate that coating blood-contacting biomedical applications with dextran is an effective route to reduce thrombo-inflammatory responses and to surface-direct biological activities.

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Section: Introductionmentioning

confidence: 99%

Human plasma protein adsorption onto dextranized surfaces: A two-dimensional electrophoresis and mass spectrometry study

Tsai

Tomczyk

Eckmann

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…Changed according to a procedure published in [ 32 ], a solution of 6% glutaraldehyde and 12% GM5 (1:1) in H 2 O was prepared and the pH was adjusted to 5.2 with 0.1 M sulfuric acid. Each PET membrane was incubated in 2 mL NaOH (1% w/v) at 37 °C for 30 min under gentle shaking and afterwards washed in deionized water to obtain a clean surface.…”

Section: Methodsmentioning

confidence: 99%

The choice of biopolymer is crucial to trigger angiogenesis with vascular endothelial growth factor releasing coatings

Claaßen

Dannecker

Grübel

et al. 2020

J Mater Sci: Mater Med

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Bio-based coatings and release systems for pro-angiogenic growth factors are of interest to overcome insufficient vascularization and bio-integration of implants. This study compares different biopolymer-based coatings on polyethylene terephthalate (PET) membranes in terms of coating homogeneity and stability, coating thickness in the swollen state, endothelial cell adhesion, vascular endothelial growth factor (VEGF) release and pro-angiogenic properties. Coatings consisted of carbodiimide cross-linked gelatin type A (GelA), type B (GelB) or albumin (Alb), and heparin (Hep), or they consisted of radically cross-linked gelatin methacryloyl-acetyl (GM5A5) and heparin methacrylate (HepM5). We prepared films with thicknesses of 8–10 µm and found that all coatings were homogeneous after washing. All gelatin-based coatings enhanced the adhesion of primary human endothelial cells compared to the uncoated membrane. The VEGF release was tunable with the loading concentration and dependent on the isoelectric points and hydrophilicities of the biopolymers used for coating: GelA-Hep showed the highest releases, while releases were indistinguishable for GelB-Hep and Alb-Hep, and lowest for GM5A5-HepM5. Interestingly, not only the amount of VEGF released from the coatings determined whether angiogenesis was induced, but a combination of VEGF release, metabolic activity and adhesion of endothelial cells. VEGF releasing GelA-Hep and GelB-Hep coatings induced angiogenesis in a chorioallantoic membrane assay, so that these coatings should be considered for further in vivo testing.

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“…A thromboresistant surface modification would therefore be of special interest. 29 In our study, we evaluated the impact of a hydrophilic surface modification (PDLLA-HEMA) on platelet adherence and shape change.…”

Section: Discussionmentioning

confidence: 99%

Glutardialdehyde induced fluorescence technique (GIFT): A new method for the imaging of platelet adhesion on biomaterials

Frank

Dresbach

Thelen

et al. 2000

J. Biomed. Mater. Res.

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One of the major limitations of biomaterials used in medicine is the adhesion and subsequent activation of platelets upon contact with blood. The development of new or modified materials necessitates adequate methods for the detection and quantification of platelet/material interactions. These interactions are commonly investigated by means of scanning electron microscopy (SEM), radioisotope and immunological techniques, or by quantification of released platelet contents. Given the lack of a simple, rapid, and inexpensive assay, we developed a novel method for the accurate assessment of platelet adhesion after contact with foreign surfaces, which enables quantitative measurements as well as imaging of the platelet shape change, and which omits conventional or immunological staining and time-consuming preparative steps. The glutardialdehyde induced fluorescence technique (GIFT) uses the epifluorescence of glutardialdehyde-fixed platelets detected by fluorescence microscopy and is suitable for opaque and transparent materials. Combined with computer-aided image analysis, numbers of adherent platelets, platelet-covered surface, and average platelet spread area can be determined as markers of surface thrombogenicity. To validate the technique, four materials of different thrombogenicity [polypropylene (PP), poly(D,L-lactide) (PDLLA), 2-hydroxyethyl-methacrylate-grafted PDLLA (PDLLA-HEMA), and heparin-coupled PDLLA-HEMA] were investigated by GIFT and SEM. We found concordant results with SEM and GIFT with the following ranking of thrombogenicity: PP > PDLLA > PDLLA-HEMA > or = PDLLA-HEMA-heparin. GIFT significantly discriminated between the investigated materials. The surface modifications led to improved thromboresistance with reduced platelet adhesion and shape change. The main advantages of GIFT as compared with SEM are: no vacuum-drying or dehydration, less time-consuming procedure, fixation and fluorescence "staining" in one step, and suitability for computer-aided image analysis allowing quantitative assessment of platelet adhesion as well as imaging of the platelet shape change with high-contrast images. In conclusion, GIFT is a valid, rapid, and simple method for the quantitative determination of platelet/material interactions intended for the evaluation of thrombogenicity of biomaterials surfaces.

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Heparin-modified polylactide as biodegradable hemocompatible biomaterial

Cited by 9 publications

References 13 publications

Human plasma protein adsorption onto dextranized surfaces: A two-dimensional electrophoresis and mass spectrometry study

Human plasma protein adsorption onto dextranized surfaces: A two-dimensional electrophoresis and mass spectrometry study

The choice of biopolymer is crucial to trigger angiogenesis with vascular endothelial growth factor releasing coatings

Glutardialdehyde induced fluorescence technique (GIFT): A new method for the imaging of platelet adhesion on biomaterials

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