1983
DOI: 10.1016/0014-5793(83)80162-8
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Heparin solubilizes asymmetric acetylcholinesterase from rat neuromuscular junction

Abstract: We are interested in the factors involved in the anchorage of acetylcholinesterase (AChE) to the synaptic basal lamina. Here, we report studies showing that heparin, a sulfated glycosaminoglycan, specifically solubilized AChE from endplate regions of rat diaphragm muscle. Of the several molecular forms of AChE present in that region, heparin only released the asymmetric Al2 and As forms of the enzyme. Our results strongly support the involvement of heparin-like macromolecules in the in vivo immobilization of t… Show more

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Cited by 47 publications
(16 citation statements)
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“…Perlecan plays important roles in the assembly and integrity of virtually all BLs, through its interactions with BLresident proteins such as type IV collagen, fibronectin, and laminins (Costell et al, 1999). In the sBL, perlecan mediates the anchoring of AChE via its collagen tail (Torres and Inestrosa, 1983;Brandan et al, 1985), as was illustrated in NMJs of perlecan-null mice, which lack AChE (Arikawa-Hirasawa et al, 2002). During myogenesis, perlecan expression is down regulated (Larraín et al, 1997a).…”
Section: Perlecanmentioning
confidence: 99%
“…Perlecan plays important roles in the assembly and integrity of virtually all BLs, through its interactions with BLresident proteins such as type IV collagen, fibronectin, and laminins (Costell et al, 1999). In the sBL, perlecan mediates the anchoring of AChE via its collagen tail (Torres and Inestrosa, 1983;Brandan et al, 1985), as was illustrated in NMJs of perlecan-null mice, which lack AChE (Arikawa-Hirasawa et al, 2002). During myogenesis, perlecan expression is down regulated (Larraín et al, 1997a).…”
Section: Perlecanmentioning
confidence: 99%
“…The latter were obtained from anesthetized female Sprague-Dawley rats (200 -250 g) by removing the diaphragm muscles together with the surrounding ribs and dissecting 2-mm strips of end plate regions (1 mm on either side of the phrenic nerve) (2,15). The end plate strips were maintained in ice-cold saline solution (0.9% NaCl).…”
Section: Methodsmentioning
confidence: 99%
“…Asymmetric AChE forms have a high binding affinity for BL components, particularly HSPGs (12) which are themselves major constituents of basement membranes (13,14). Heparin and heparan sulfate have also been shown to release asymmetric AChE activity from rat muscle end plate regions (15) and BL sheets purified from the electric organ of Discopyge (16). The demonstration that A 12 could bind and be selectively eluted from heparin-agarose columns, whereas non-collagenous forms and A 12 after collagenase treatment could not, proved the direct interaction of A 12 with heparin in vitro (11,17).…”
mentioning
confidence: 99%
“…Among glycosaminoglycans (GAGS), heparan sulfate has been singled out as the most likely candidate for this role in view of the proven affinity of the tailed AChE species for heparin [3][4][5][6]8], and of the reported ability of heparinases (but not chondroitinases) to dissociate aggregative AChE-GAG complexes [2], and to release asymmetric AChE from electric organ samples [S]. Our recent work has shown, however, that the affinity of heparin for the asymmetric AChE tail is not a selective phenomenon, being shared by a number of polyanions including sulfated GAGS, dextran sulfates, some acidic polyaminoacids and even polyvinylsulfates [9].…”
Section: Introductionmentioning
confidence: 99%