Heparin-Sorbent for Low Density Lipoproteins Removal in Hypercholesterolemia

Abstract

Heparin-sorbent was developed in the USSR Cardiology Research Center for LDL-apheresis in patients with familial hypercholesterolemia. Clinical trials of the sorbent were started in 1985. Heparin-sorbent has a high capacity for LDL. One column (400ml) can remove 3 - 6g of LDL cholesterol, depending on the initial LDL content in the plasma. The sorbent demonstrates a high stability, it can withstand more then 75 sorbtion-desorbtion cycles with only a slight loss of the initial capacity. It means that one column can work for 1,5-2,0 years when used weekly. Heparin-sorbent is the sorbent of limited selectivity, apo-B content in eluate is 40-60% of the total protein content, but it is important that it does not bind HDL.

Immunoadsorption of Cholesterol on Protein A Oriented Beads

“…Their maximum LDL binding capacities were in the range of 0.6–2.5 mg per milliliter of adsorbent. Sinitsyn et al used Sepharose beads carrying heparin and they reported adsorption values of up to 12 mg of cholesterol per gram of polymer 32. In this study, we demonstrated that random and oriented anti‐LDL immobilized poly(HEMA‐EGDMA) adsorbents exhibit comparable cholesterol binding capacities with bioaffinity adsorbents including commercial media.…”