Hepatic and Haemato-biochemical Alterations in Juvenile Mozambique tilapia (Oreochromis mossambicus) on Pawpaw (Carica papaya) Seed Meal

Abstract

Pawpaw seed meal (PSM) can be used as an antifertility agent in animals, however there is no information on its potential toxicological effect on Mozambique tilapia (O. mossambicus). In this study the effect of PSM on the liver, blood haematological and biochemical parameters of O. mossambicus juveniles was assessed to ascertain its suitability as a reproductive suppressant. The PSM was administered at an inclusion level of 10 g and 30 g/kg of a commercial tilapia diet (basal diet, BD) for 30 and 60 days, respectively. The potential toxicological effect was assessed by determining the extent of change in the normal haematological (RBC count, haematocrit, haemoglobin, MCV, MCH, MCHC, thrombocyte count, absolute WBC and differential cell counts) and biochemical (cholesterol, total protein, albumin, globulin and glucose) parameters. Haematological and biochemical profiles of different treatment groups did not differ throughout the course of the investigation. Liver weight and hepatosomatic index values of the treated fish were comparable to those of the control. The absence of any effect of the blood parameters measured in this study also indicate that PSM has no compromising influence on the immune system of the fish, indicating that PSM can be considered as a safe alternative to induce masculinization in Mozambique tilapia, and therefore act as a reproductive inhibitor.

Biomarker and hematological fieldwork with amphibians: is it necessary to sample all night?

“…This result is different from previous reports available in amphibian species where, when present, sex differences in leukocytes were in terms of cell type proportions and not at the levels of total WBC counts (Mahapatra et al 2012;Meesawat et al 2016;Xiong et al 2018). However, similar sex-related differences in total WBC counts have been reported in fish (Motlagh et al 2012;George and Akinrotimi 2017;Omeje et al 2019). With respect to erythrocyte-related parameters such as PCV, HB, MCV, and MCH, the current study on L. latrans did not find any sexrelated differences in contrast to previous studies with other amphibian species which, in most cases, identified one or two sex-dependent parameters.…”

Addition of essential oil from lemongrass to the tambaqui ( Colossoma macropomum ) diet: Effects on growth, intestinal enzymes, haematological and metabolic variables, and antimicrobial challenge

“…Nevertheless, we believe liver hyperplasia and hypertrophy did not occur in these treatments, as the HSI was reduced. The decrease in the HSI improves the hepatic response related to nutrition, such as carbohydrate, protein and lipid metabolism, and storage of glycogen, vitamins and iron (Omeje et al, 2019).…”