Hepatic cytoprotective effect of ischemic and anesthetic preconditioning before liver resection when using intermittent vascular inflow occlusion: A randomized clinical trial

Rodríguez, A.; Taurà, Pilar; Domingo, María Isabel García; Herrero, E.; Camps, Judith; Forcada, Pilar; Sabaté, Sergi; Cugat, E.

doi:10.1016/j.surg.2014.09.005

Cited by 29 publications

(15 citation statements)

References 39 publications

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“…However, effective prevention and treatment are still lacking. Anesthetic preconditioning is a promising strategy that has been widely studied in IRI of various organs [34,35]. Anesthesia has been found to significantly attenuate acute liver inflammation induced by lipopolysaccharide/D-galactosamine [16], which raises the intriguing question of whether sufentanil contributes to alleviating hepatic IRI, since inflammation functions as an important initiator and progresses through the entire process.…”

Section: Discussionmentioning

confidence: 99%

Sufentanil Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury by Suppressing Inflammation

et al. 2019

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Background Inflammation is one of the most significant mechanisms of hepatic ischemia-reperfusion injury (IRI). Sufentanil has a protective effect against liver injury by reducing inflammatory response. In this study, we used a cellular hepatic ischemic/reoxygenated (IR) model to determine whether sufentanil preconditioning protects against hepatic IRI. Material/Methods The human normal liver cells line L-O2 was studied. The levels of glutamic oxaloacetic transaminase (AST), lactate dehydrogenase (LDH), malonaldehyde (MDA), and superoxide dismutase (SOD) were measured using corresponding assay kits. The protein levels of total and phosphorylated ERK1/2, JNK, and p38, and the expression of p65 and COX2 genes, were measured by Western blotting. The levels of inflammatory factors were examined by ELISA. The Cell Counting Kit-8 (CCK-8) was used to determine if the viability of L-O2 cells was affected by sufentanil. The effects of sufentanil on IR-induced cell apoptosis were examined by flow cytometry. Results IR-induced caused L-O2 cells to become rounded and to have a lower adhesive rate than normal cells. The levels of AST, LDH, and MDA were higher but the level of SOD was lower in the IR group than in the control group. The phosphorylated protein levels of ERK1/2, JNK, and p38, along with the expression of p65 and COX2, were upregulated in the IR group compared to the normal group. In addition, a variety of inflammatory factors were secreted in L-O2 cells after IR. The viability of L-O2 cells decreased and cell apoptosis increased significantly after IR treatment. All indexes of cell injury were reversed by sufentanil in a concentration-dependent manner. Conclusions Sufentanil stimulation triggers downregulation of inflammatory factors such as HIF-1α, TNF-α, IL-1β, and IL-6, possibly through suppressing the p38/ERK/JNK/NF-κB-p65/COX2 pathways, and thereby reduces the damage to IR hepatic cells.

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Section: Discussionmentioning

confidence: 99%

Sufentanil Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury by Suppressing Inflammation

et al. 2019

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“…Liver paraffin-embedded, standard haematoxylin-eosin stained sections were analyzed. According to Rodriguez four elements of the liver histology were analyzed: steatosis (micro vesicular and macro vesicular) in 4 grades, degree of sinusoidal congestion and dilatation in 3 grades, leukocyte infiltration in 3 grades and necrosis (focal, confluent, or zonal) in 3 grades (26). Each biopsy was evaluated by a single pathologist blinded to the treatment allocation.…”

Section: Methodsmentioning

confidence: 99%

Comparison of hepatoprotective effect from ischemia-reperfusion injury of remote ischemic preconditioning of the liver vs local ischemic preconditioning of the liver during human liver resections

Rakić

Patrlj

Amić

et al. 2018

International Journal of Surgery

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“…From bench to bedside, a randomized clinical trial (RCT) involves 60 liver donors by 10 min inflow occlusion and 10 min reperfusion to improve the graft tolerance to IRI, and the results indicate that LIPC approach significantly improves liver biochemical markers of hepatocyte function in deceased donor liver transplantation (LT) [ 6 ]. However, another similar clinical study with smaller sample size has not shown any difference between LIPC and control group [ 7 ] nor has any beneficial result of LIPC been seen in liver resection surgery [ 8 ]. These controversial results may arise from invasive procedure to induce LIPC, in which direct vessel clamping may cause uncontrollable degree of graft injury and thus contribute to varied clinical results.…”

Section: Antioxidant Treatment For Donormentioning

confidence: 99%

Current Antioxidant Treatments in Organ Transplantation

Shi

Xue

2016

Oxidative Medicine and Cellular Longevity

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Oxidative stress is one of the key mechanisms affecting the outcome throughout the course of organ transplantation. It is widely believed that the redox balance is dysregulated during ischemia and reperfusion (I/R) and causes subsequent oxidative injury, resulting from the formation of reactive oxygen species (ROS). Moreover, in order to alleviate organ shortage, increasing number of grafts is retrieved from fatty, older, and even non-heart-beating donors that are particularly vulnerable to the accumulation of ROS. To improve the viability of grafts and reduce the risk of posttransplant dysfunction, a large number of studies have been done focusing on the antioxidant treatments for the purpose of maintaining the redox balance and thereby protecting the grafts. This review provides an overview of these emerging antioxidant treatments, targeting donor, graft preservation, and recipient as well.

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Hepatic cytoprotective effect of ischemic and anesthetic preconditioning before liver resection when using intermittent vascular inflow occlusion: A randomized clinical trial

Cited by 29 publications

References 39 publications

Sufentanil Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury by Suppressing Inflammation

Sufentanil Preconditioning Protects Against Hepatic Ischemia-Reperfusion Injury by Suppressing Inflammation

Comparison of hepatoprotective effect from ischemia-reperfusion injury of remote ischemic preconditioning of the liver vs local ischemic preconditioning of the liver during human liver resections

Current Antioxidant Treatments in Organ Transplantation

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