Hepatic differentiation of murine embryonic stem cells

Jones, E

doi:10.1016/s0168-8278(01)80082-9

Cited by 18 publications

(24 citation statements)

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“…Previous studies with murine ES cells showed that several types of cells could be enriched in culture either by addition of growth factors [12][13][14][15][16] or by introduction of transcription factors. 17 Whereas the conditions that direct differentiation of mouse ES cells into neuronal, hematopoietic or heart muscle cells have been published, [12][13][14][15][16] the differentiation of mouse ES cells into hepatocyte was reported only recently. [18][19][20][21] The most effective therapy for acute or chronic hepatic failure is orthotopic liver transplantation (OLT), which still holds the best therapeutic promise for thousands of patients worldwide each year.…”

Section: Discussionmentioning

confidence: 99%

“…Here we have shown that mouse ES cells can give rise to hepatocyte-like cell clustering in vitro and in vivo . In many studies, growth factors or transcription factors have been added, [12][13][14][15][16][17] but we consider that cell-to cell contact is more important in ES cell differentiation 10 because cell-to-cell contact is critical in increasing the many factors that are essential for ES cells differentiation. Some factors are known, but many others are unknown.…”

Section: Discussionmentioning

confidence: 99%

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Differentiation of mouse embryonic stem cells into hepatocytes in vitro and in vivo

Kuai

Bian

Cong³

et al. 2003

Chinese Journal of Digestive Diseases

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OBJECTIVE: Embryonic stem (ES) cells have a pluripotent ability to differentiate into a variety of cell lineages. Cell‐to‐cell contact is important for cell differentiation. Mouse ES cells were cocultured with mouse fetal liver cells and the green fluorescent protein (GFP) positive ES cells were transplanted into rats liver through the portal vein in order to investigate their potential to differentiate into hepatocytes. METHODS: Mouse ES cells were cocultured with the mouse fetal liver cell line, BNL.CL2. They did not make direct contact; instead the culture media was exchanged freely. After coculture for 48 h, albumin, transthyretin, glucose 6 phosphates, hepatic nuclear factor 4 and SEK1 mRNA were assayed by RT‐PCR, and alpha‐fetoprotein by immunohistochemistry. The morphology was investigated by microscopy. After transplantion of the GFP‐positive ES cells, the whole liver was removed from a rat every four days. The liver slices were examined under a fluorescent microscope to detect the GFP‐positive cells. Albumin was detected on the same slices by immunohistochemistry. RESULTS: After coculture with BNL.CL2 cells, the differentiated ES cells had the same morphology as the BNL.CL2 cells, and albumin, transthyretin, glucose 6 phosphates and SEK‐1 mRNA were found by RT‐PCR, and alpha‐fetoprotein was detected immunohistochemically. The transplanted GFP‐positive ES cells were found in the rats’ liver slices by GFP fluorescence, and development of teratomas was not observed. The immunohistochemistry results indicated that the transplanted GFP‐positive ES cells retained an albumin‐producing ability. CONCLUSIONS: Cell‐to‐cell contact is important for the differentiation of ES cells. Mouse embryonic stem cells can differentiate into hepatocytes directly either in vitro or in vivo.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differentiation of mouse embryonic stem cells into hepatocytes in vitro and in vivo

Kuai

Bian

Cong³

et al. 2003

Chinese Journal of Digestive Diseases

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“…To be able to specifically monitor hepatocyte development and isolate these cells from the differentiation cultures, several studies have incorporated approaches to distinguish definitive endoderm and early liver populations from visceral endoderm. Jones et al 22 used an ESC line carrying a lacZ gene trap vector inserted in a gene known as Gtar. Gtar is expressed in definitive endoderm committed to a hepatic fate and the developing liver but is not found in visceral endoderm.…”

Section: Differentiating Hepatocyte-lineages From Esc In Vitromentioning

confidence: 99%

Human embryonic stem cells and liver diseases: From basic research to future clinical application

Wang

Lü

Wang

et al. 2008

J of Digest Diseases

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Human embryonic stem cells (hESC) provide access to the earliest stages of human development and because of their high proliferation capability, pluripotency and low immunogenicity may serve as a potential source of specialized cells for regenerative medicine. hESC-derived hepatocyte-like cells exhibit characteristic hepatocyte morphology, express hepatocyte markers and are capable of executing a range of hepatocyte functions. However, there are many challenges and obstacles to be overcome before the use of hESC and hESC-derived hepatocyte-like cells in clinical practice can be realized. Here, we highlight some of the recent efforts in this area, in hope of providing insights toward this complex yet important area of therapeutical modality for treating patients with liver disease.

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“…The expression patterns of endodermal marker genes were examined in spontaneously differentiated HNF4overexpressing ES cells by RT-PCR; specifically, the primers for genes that were expressed in endodermderived tissues, which were albumin, transthyretin, G-6-P and SEK-1. 8,[16][17][18][19] In addition β-actin gene was used as a control for baseline RNA quantities. The results are summarized in Fig.…”

Section: Expression Of Endoderm Structural Genes In Spontaneously Difmentioning

confidence: 99%

Treatment of surgically induced acute liver failure by transplantation of HNF4‐overexpressing embryonic stem cells

Kuai

Cong²,

Du³

et al. 2006

Chinese Journal of Digestive Diseases

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Hepatic differentiation of murine embryonic stem cells

Cited by 18 publications

References 0 publications

Differentiation of mouse embryonic stem cells into hepatocytes in vitro and in vivo

Differentiation of mouse embryonic stem cells into hepatocytes in vitro and in vivo

Human embryonic stem cells and liver diseases: From basic research to future clinical application

Treatment of surgically induced acute liver failure by transplantation of HNF4‐overexpressing embryonic stem cells

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