2020
DOI: 10.3390/bioengineering7020047
|View full text |Cite
|
Sign up to set email alerts
|

Hepatic Differentiation of Stem Cells in 2D and 3D Biomaterial Systems

Abstract: A critical shortage of donor livers for treating end-stage liver failure signifies the urgent need for alternative treatment options. Hepatocyte-like cells (HLC) derived from various stem cells represent a promising cell source for hepatocyte transplantation, liver tissue engineering, and development of a bioartificial liver assist device. At present, the protocols of hepatic differentiation of stem cells are optimized based on soluble chemical signals introduced in the culture medium and the HLC produced typi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
22
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 22 publications
(22 citation statements)
references
References 106 publications
0
22
0
Order By: Relevance
“…In addition, the stiffness of biomaterials is one of the most important factors for the differentiation of stem cells, with soft biomaterials affording better promotion of homogeneous differentiation into HLCs and hepatic maturation compared to stiffer biomaterials or a tissue culture dish. The stiffness (Young’s modulus) of a soft hydrogel was calculated from the elasticity ( G ′) and viscosity ( G ″) obtained using a rheometer. , The stiffness of 50 mg/mL HYDROX after 3 h of exposure to phosphate-buffered saline was 19.7 ± 6.5 Pa by calculation based on G ′ and G ″. At 24 h after addition of the solution, however, 50 mg/mL HYDROX was in a sol state (Figure S2), and thus the stiffness of 50 mg/mL HYDROX could not be estimated after this time point.…”
Section: Resultsmentioning
confidence: 99%
“…In addition, the stiffness of biomaterials is one of the most important factors for the differentiation of stem cells, with soft biomaterials affording better promotion of homogeneous differentiation into HLCs and hepatic maturation compared to stiffer biomaterials or a tissue culture dish. The stiffness (Young’s modulus) of a soft hydrogel was calculated from the elasticity ( G ′) and viscosity ( G ″) obtained using a rheometer. , The stiffness of 50 mg/mL HYDROX after 3 h of exposure to phosphate-buffered saline was 19.7 ± 6.5 Pa by calculation based on G ′ and G ″. At 24 h after addition of the solution, however, 50 mg/mL HYDROX was in a sol state (Figure S2), and thus the stiffness of 50 mg/mL HYDROX could not be estimated after this time point.…”
Section: Resultsmentioning
confidence: 99%
“…On the flip side, the current differentiation protocols only achieve partial differentiation 47,48 . Specifically, HLCs display strongly (up to 1000‐fold) increased AFP expression (a marker of foetal, immature hepatocytes), whereas HNF4A and ALB levels (markers of fully differentiated hepatocytes) are 10‐ to 100‐fold lower than in mature human hepatocytes, thus suggesting “only” foetal gene expression patterns that resemble dedifferentiated hepatocytes in 2D culture 49‐51 .…”
Section: Liver Spheroid Applications In Drug Discovery and Developmentmentioning
confidence: 99%
“…Differentiation strategies are vital for hepatocyte derivation and in developing scaffold‐based liver tissue 5 . The most widely used differentiation strategy involves the utilization of defined cocktail of growth factors for 21–28 days in a stage‐specific manner to derive functional hepatocytes 6 .…”
Section: Introductionmentioning
confidence: 99%
“…Differentiation strategies are vital for hepatocyte derivation and in developing scaffold-based liver tissue. 5 The most widely used differentiation strategy involves the utilization of defined cocktail of growth factors for 21-28 days in a stage-specific manner to derive functional hepatocytes. 6 The major setbacks with such approaches are the cost and the need for the longer time duration to derive functional hepatocytes for its abundant requirements in clinical applications.…”
mentioning
confidence: 99%
See 1 more Smart Citation