Hepatic expression of cytochrome P450 enzymes in non‐human primate species

Uehara, Shotaro; Uno, Yasuhiro; Yamazaki, Hiroshi

doi:10.1111/jmp.12288

J of Medical Primatology

2017

DOI: 10.1111/jmp.12288

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Hepatic expression of cytochrome P450 enzymes in non‐human primate species

Shotaro Uehara

Yasuhiro Uno

Hiroshi Yamazaki

Abstract: Cytochromes P450 (P450) largely remain to be characterized in great apes. Comparative immunochemical detection of drug metabolizing forms of P450s 1A, 2A, 2B, 2C, 2D, 2E, 2J, 3A, 4A, and 4F in liver microsomes from chimpanzees, gorillas, orangutans, gibbons, cynomolgus and rhesus macaques, and common marmosets were carried out.

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2022

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Cited by 1 publication

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Metabolism of gartanin in liver microsomes and its modulating effects on cytochrome P450s

Jia

2022

Xenobiotica

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The retention time (11.20 min), [M-H] − and MS/MS spectra of the molecular ion at m/z 395.0658 (M0) were the same as those of the gartanin standard. Therefore, M0 was confirmed as the unchanged parent drug. Gartanin gave the characteristic fragment ions at m/z 351.0123, 339.0141, and 296.9763. The cleavage of gartanin at C-22 and C-17 produced a daughter ion at m/z 351, which created further fragment ions at m/z 339.0141. However, the structure of the fragment ion at m/z 296.9763 was not inferred. M1 and M2The molecular formulas of M1 (tR = 9.42 min) and M2 (tR = 10.21 min) were determined as C23H24O7 with the [M-H] − at m/z 411.0722 and 411.0719, respectively.Their m/z was 16 Da higher than that of gartanin, indicating that they were the hydroxylated metabolite of the parent drug. The hydroxyl group was added to the parent molecule at C-18(19) or C-22(23). Based on the MS/MS spectra of M1 and M2, two side chains underwent a series of cleavage at C-22, C-21, and C-17 and generated the product ion at m/z 381.0663, 351.0247, and 337.0116. The m/z 351 fragment was the same as gartanin, due to cleavage of the isoprenyl group, indicating the new

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Metabolism of gartanin in liver microsomes and its modulating effects on cytochrome P450s

Jia

2022

Xenobiotica

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Hepatic expression of cytochrome P450 enzymes in non‐human primate species

Cited by 1 publication

References 19 publications

Metabolism of gartanin in liver microsomes and its modulating effects on cytochrome P450s

Metabolism of gartanin in liver microsomes and its modulating effects on cytochrome P450s

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