Hepatic fibropoiesis in dogs naturally infected with Leishmania (Leishmania) infantum treated with liposome-encapsulated meglumine antimoniate and allopurinol

Canine Visceral Leishmaniasis: A Histological and Immunohistochemical Study of Fibropoiesis in Chronic Interstitial Pneumonitis

“…TGF-β is the central cytokine in various fibrotic diseases, and it is released by cell sinusoidal capillaries, endothelial cells, CD4 T lymphocytes, monocytes, macrophages, hepatocytes, and bronchial epithelial cells [25][26][27]. We showed the expression of this cytokine in the liver related to the fibrosis process in experimentally and naturally L. infantum-infected dogs [15,27,28]. Here, we found high TGF-β expression in bronchial epithelial cells, as well as in areas of hypertrophy and hyperplasia of pneumocytes and in the intra-alveolar space (pulmonary interstitium).…”

“…Ecadherin and cytokeratin were also investigated as epithelial cell markers. It is known that decreasing E-cadherin expression along with increasing Snail expression indicates EMT during fibrogenesis [28]. Thus, upregulation of the expression of TGF-β, Snail, vimentin, and α-SMA, in contrast to the low E-cadherin expression (no difference among groups), might suggest the activity of mesenchymal cells derived from EMT.…”

Extra virgin coconut oil (Cocos nucifera L.) intake shows neurobehavioural and intestinal health effects in obesity-induced rats

“…Colon sections were performed and pre-treated for antigenic recovery using a 0.1% citrate retriever buffer solution [pH 6.0] (DAKO®, Vila Real Carpinteria, CA, code S1699) in a high temperature method and incubated in a water bath for 30 min at 97 °C. Monoclonal antibodies were used as primary antibodies as follows: (MΦ) anti-calprotectin (L1 antigen) clone MAC387 (DAKO), dilution 1 : 100; 44 anti-macrophage (MΦ) anti-CD163 clone ED2, (Santa Cruz Biotechnology), 1 : 100. 45…”

“…37 Colon sections were performed and pre-treated for antigenic recovery using a 0.1% citrate retriever buffer solution [ pH 6.0] (DAKO®, Vila Real Carpinteria, CA, code S1699) in a high temperature method and incubated in a water bath for 30 min at 97 °C. Monoclonal antibodies were used as primary antibodies as follows: (MΦ) anti-calprotectin (L1 antigen) clone MAC387 (DAKO), dilution 1 : 100; 44 anti-macrophage (MΦ) anti-CD163 clone ED2, (Santa Cruz Biotechnology), 1 : 100. 45 All slides for the morphometric analysis were incubated 18-22 h at 4 °C in a humid chamber, washed in PBS, incubated with biotinylated goat anti-mouse and anti-rabbit antibodies (LSAB2 kit; Dako), washed with PBS, and incubated with streptavidin-peroxidase complex (LSAB2 kit; Dako) for 20 min at room temperature (±26 °C).…”

Resposta imune-inflamatória a agentes infecciosos no microambiente hepático de cães e gatos: revisão

“…Este processo inflamatório granulomatoso leva à fibropoiese hepática intensa nos cães naturalmente infectados com L. (Leishmania) infantum (Melo et al, 2009). O aumento na deposição de colágeno tecidual se deve a maior expressão de TGF-β pelas células do granuloma e, então, ativação das CEH (Castro et al, 2018;Madeira et al, 2016).…”