“…Strikingly, we also found that oscillatory fed-microRNAs, which inhibit the expression of catabolic genes and consequently enable fasted-refed transition, are expressed in anticipation during a starvation state (Maniyadath et al, 2019). Albeit parallel studies have also illustrated circadian dependence or rhythmicity in the expression of microRNAs across tissues, including the liver (Menet et al, 2012;Vollmers et al, 2012;Ji et al, 2023), whether fed-fast cycles contribute to miR biogenesis independently or cooperatively (with circadian inputs) remains unknown. In this context, we have delineated the causal upstream cues that dictate the anticipatory expression of hepatic fed-microRNAs, especially for let-7i, mir-204, mir-221, and mir-222, which not only displayed the most robust oscillation but also otherwise have been shown to be important for liver functions and hepatic cancers (Chen et al, 2014;Luo et al, 2017;Song et al, 2017b).…”