Hepatic gene expression profiling using Genechips in zebrafish exposed to 17α-ethynylestradiol

Hoffmann, Jennifer L.; Torontali, S.P.; Thomason, Ryan; Lee, D.M.; Brill, Jessica L.; Price, Bradford B.; Carr, Gregory J.; Versteeg, Donald J.

doi:10.1016/j.aquatox.2006.06.009

Cited by 100 publications

(53 citation statements)

References 51 publications

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“…Numerous previous studies have examined changes in gene transcription in the liver or ovary of fish exposed to xenoestrogens [3][4][5][6][7] and xenoandrogens [8,9]. Fewer studies have examined the influence of endocrine-disrupting compounds on neuroendocrine function, despite the importance of the brain and pituitary for normal reproductive function [10,11].…”

Section: Introductionmentioning

confidence: 96%

Relationships between the transcriptome and physiological indicators of reproduction in female rainbow trout over an annual cycle

Hook

Nagler

Cavileer

et al. 2010

Enviro Toxic and Chemistry

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Normal transcriptomic patterns along the brain-pituitary-gonad-liver (BPGL) axis should be better characterized if endocrine-disrupting compound-induced changes in gene expression are to be understood. Female rainbow trout were studied over a complete year-long reproductive cycle. Tissue samples from pituitary, ovary, and liver were collected for microarray analysis using the 16K Genomic Research on Atlantic Salmon Project (GRASP) microarray and for quantitative polymerase chain reaction measures of estrogen receptor (ER) isoform messenger RNA (mRNA) levels. Plasma was collected to determine levels of circulating estradiol-17β (E2), follicle-stimulating hormone (FSH), and luteinizing hormone (LH). As an a priori hypothesis, changes in gene expression were correlated to either circulating levels of E2, FSH, and LH, or ER mRNAs quantified by quantitative polymerase chain reaction. In the liver, most transcriptomic patterns correlated to levels of either E2, LH, or ERs. Fewer ovarian transcripts could be correlated to levels of E2, ERα, or FSH. No significant associations were obvious in the pituitary. As a post hoc hypothesis, changes in transcript abundance were compared with microarray features with known roles in gonadal maturation. Many altered transcripts in the ovary correlated to transcript levels of estradiol 17-beta-dehydrogenase 8 or 17 B HSD12, or to glycoprotein alpha chain 1 or 2. In the pituitary, genes involved with the growth axis (e.g., growth hormone, insulin-related growth factor binding protein) correlated with the most transcripts. These results suggest that transcriptional networks along the BPGL axis may be regulated by factors other than circulating steroid hormones.

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Section: Introductionmentioning

confidence: 96%

Relationships between the transcriptome and physiological indicators of reproduction in female rainbow trout over an annual cycle

Hook

Nagler

Cavileer

et al. 2010

Enviro Toxic and Chemistry

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show abstract

“…Microarray analysis has also been performed during early developmental stages (1, 2, 3 and 4 dpf). In addition to well-known Er target genes like vtg1 (the only gene induced by estrogens at all stages studied [32]), vtg3, vtg4 and er , these studies also revealed Er regulation of many metabolic genes [36,37] and genes involved in cell cycle and DNA repair [38].…”

Section: The Zebrafish Ersmentioning

confidence: 99%

“…The liver and telencephalon transcriptome have been determined using microarray analysis of adult male and female fish after exposure to EE2 [36,37]. Whole doi:10.11131/2017/101286body transcriptome analysis was performed on male adult fish after exposure to E2 in another microarray study [38].…”

Section: The Zebrafish Ersmentioning

confidence: 99%

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

Schaaf¹

2017

Nuclear Receptor Research

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Abstract. Steroid hormones regulate a wide range of processes in our body, and their effects are mediated by steroid receptors. In addition to their physiological role, these receptors mediate the effects of endocrine disrupting chemicals (EDCs) and are widely used targets for dugs involved in the treatment of numerous diseases, ranging from cancer to inflammatory disorders. Over the last fifteen years, the zebrafish has increasingly been used as an animal model in steroid receptor research. Orthologues of all human steroid receptor genes appear to be present in zebrafish. All zebrafish steroid receptors have been characterized in detail, and their expression patterns have been analyzed. Functional studies have been performed using morpholino knockdown of receptor expression and zebrafish lines carrying mutations in one of their steroid receptor genes. To investigate the activity of the receptors in vivo, specific zebrafish reporter lines have been developed, and transcriptomic studies have been carried out to identify biomarkers for steroid receptor action. In this review, an overview of research on steroid receptors in zebrafish is presented, and it is concluded that further exploitation of the possibilities of the zebrafish model system will contribute significantly to the advancement of steroid receptor research in the next decade.

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“…The effects on %HSI and %GSI were consistent with the etiology of EE2's disruptive effects. Hoffmann et al [2006] have shown an almost 66% reduction in GSI for female zebrafish exposed to 98 ng/L EE2 (7 day exposure), whereas Van den Belt et al [2004] showed $550% reduction in GSI for female zebrafish exposed to 8.6 ng/L EE2 (or 0.029 nM) for 3 weeks. In contrast to a reduction of %GSI (for females only), EE2 exposure significantly enhanced %HSI for both male and female fathead minnows in our study.…”

Section: Effects On Morphometric Indicesmentioning

confidence: 99%

“…Sexually mature and reproductively active male and female fathead minnows were exposed to 100 ng/L EE2 for ten days. Although the exposure concentration selected was above levels commonly detected in the aquatic environment (i.e., up to 10 ng/L), it was within range shown to significantly impact reproduction and gonad growth in fish ($1 to 100 ng/L) [Hoffmann et al 2006;Santos et al 2010;Scholz and Gutzeit 2000]. A previously developed stoichiometric model of piscine steroidogenesis [Hala et al 2010[Hala et al , 2012 was expanded upon and augmented with a pseudo 'biomass reaction' detailing the concentrations of experimentally determined steroid hormone metabolites (using mass spectrometry) comprising per unit gonad weight (as fmol/mg tissue) of male and female fish.…”

Section: Introductionmentioning

confidence: 99%

In Silicoanalysis of perturbed steroidogenesis and gonad growth in fathead minnows (P. promelas) exposed to 17α-ethynylestradiol

Hala¹,

Petersen²,

Martinović³

et al. 2015

Systems Biology in Reproductive Medicine

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Hepatic gene expression profiling using Genechips in zebrafish exposed to 17α-ethynylestradiol

Cited by 100 publications

References 51 publications

Relationships between the transcriptome and physiological indicators of reproduction in female rainbow trout over an annual cycle

Relationships between the transcriptome and physiological indicators of reproduction in female rainbow trout over an annual cycle

The First Fifteen Years of Steroid Receptor Research in Zebrafish; Characterization and Functional Analysis of the Receptors

In Silicoanalysis of perturbed steroidogenesis and gonad growth in fathead minnows (P. promelas) exposed to 17α-ethynylestradiol

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