Hepatic Helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis

Fox, James G.; Dewhirst, Floyd E.; Shen, Zeli; Feng, Yan; Taylor, Nancy S.; Paster, Bruce J.; Ericson, Rebecca L.; Lau, C. N.; Correa, Pelayo; Araya, Juan Carlos; Roa, Iván

doi:10.1016/s0016-5085(98)70589-x

Cited by 445 publications

(417 citation statements)

References 41 publications

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“…Colonization with Helicobacter sp. was confirmed 2 weeks post-inoculation by PCR analysis of feces using Helicobacter genus specific primers (20). C57BL IL10 −/− mice were necropsied at 6 and 10 weeks p.i, and feces were collected followed by liver, stomach, cecum and colon for culture, quantitative PCR, RNA isolation and histology.…”

Section: Experimental Infection Of C57bl/129 Il 10 −/− Micementioning

confidence: 99%

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice

Shen¹,

Feng²,

Muthupalani³

et al. 2016

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A novel Helicobacter species Helicobacter japonicum was isolated from the stomach and intestines of clinically normal mice received from three institutes from Japan. The novel Helicobacter sp. was microaerobic, grew at 37°C and 42°C, was catalase and oxidase positive, but urease negative. It is most closely related to the 16S rRNA gene of H.muridarum (98.6%); to the 23S rRNA gene of H.hepaticus (97.9%); to the hsp60 gene of H.typhlonius (87%). The novel Helicobacter sp. has in vitro cytolethal distending toxin (CDT) activity; its cdtB gene sequence has 83.8% identity with that of H.hepaticus. The whole genome sequence of H.japonicum MIT 01-6451 has a 2.06-Mb genome length with a 37.5% G + C content. When the organism was inoculated into C57BL/129 IL10 −/− mice, it was cultured from the stomach, colon and cecum of infected mice at 6 and 10 weeks post-infection. The cecum had the highest H.japonicum colonization levels by quantitative PCR. The histopathology of the lower bowel was characterized by moderate to severe inflammation, mild edema, epithelial defects, mild to severe hyperplasia, dysplasia and carcinoma. Inflammatory cytokines IFNγ, TNFα and IL17a, as well as iNOS were significantly upregulated in the cecal tissue of infected mice. These results demonstrate that the novel H.japonicum can induce inflammatory bowel disease and carcinoma in IL10 −/− mice and highlights the importance of identifying novel Helicobacter spp. especially when they are introduced from outside mouse colonies from different geographic locations.

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Section: Experimental Infection Of C57bl/129 Il 10 −/− Micementioning

confidence: 99%

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice

Shen¹,

Feng²,

Muthupalani³

et al. 2016

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“…Enterohepatic Helicobacter spp. have also been identi®ed recently in bile and gall bladder tissue from patients with chronic cholecystitis [21]. However, little is known about virulence factors of these newly discovered bacteria and pathogenic mechanisms involved in inducing gastrointestinal diseases.…”

Section: Introductionmentioning

confidence: 99%

Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.

Chien

Taylor

et al. 2000

Journal of Medical Microbiology

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110

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A bacterial toxin that causes progressive distension and death of Chinese hamster ovary (CHO) cells and HeLa cells, termed cytolethal distending toxin (Cdt), has been identi®ed in several diarrhoeagenic bacteria, including Campylobacter spp. (C. jejuni and C. coli), some pathogenic strains of Escherichia coli and Shigella spp. Genes encoding this toxin were identi®ed as a cluster of three adjacent genes cdtA, cdtB and cdtC. Homologues of cdtB from ®ve species of enterohepatic helicobacters ( Helicobacter hepaticus, H. bilis, H. canis and two novel Helicobacter spp. isolated from mice and woodchuck, respectively) were identi®ed by means of degenerative PCR primers, cloned and sequenced. The similarities of these partial cdtB nucleotide sequences from these Helicobacter spp. to those of cdtB genes known to be present in other bacteria were: C. jejuni, 58.3±64.8%; E. coli, 52.3±57.8%, Haemophilus ducreyi, 53.4±58.4% and Actinobacillus actinomycetemcomitans, 52.7±58.1%. Bacterial lysates from four of these helicobacters caused characteristic cytolethal distension of HeLa cells. Cdt caused cell cycle arrest at G 2 /M phase as measured by¯ow cytometry. The results demonstrated the presence of a toxin in these Helicobacter spp. belonging to the family of Cdt.

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“…From reports in literature 9,15,16 referring about the isolation of helicobacter species in bile from the liver and particularly of the gallbladder and its implications in the pathogenesis of diseases, we became interested to assess the prevalence of Helicobacter spp in patients operated on for chronic cholecystitis .…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder

et al. 2010

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Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder 1Prevalência do Helicobacter spp na colecistite crônica calculosa e correlação com as alterações histológicas da vesícula biliar ABSTRACT Purpose: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with the gallbladder's histological findings. Methods: 100 patients were operated for chronic cholecystitis with cholecystolithiasis. In pathological examination of the gallbladder, were evaluated the presence of metaplasia, dysplasia, lymphoid follicles, anaplasia and tumors that might be related to the presence of Helicobacter plus the presence of the bacilli Giemsa by optical microscopy. From the DNA extracted from the gallbladder's bile, PCR was performed by using specific primers for the identification of Helicobacter spp with amplification of the 400bp segment of rRNA gene16S, with positive control DNA from Helicobacter pylori. All the cases negative for isolation of genetic material were excluded. The cases of PCR and Giemsa were used as negative control group. The histological findings were compared to the presence of bacilli and PCR data using a chi-square and Fisher's Exact test (CI = 95.0%, p <0.05). Results: Of 68 patients, 42 (61.8%) were PCR for Helicobacter spp and 19 (27.9%) had Giemsa . There was no correlation between the two findings. The PCR for Helicobacter spp was not correlated to the histological findings. The presence of lymphoid follicles and metaplasia was related to the Giemsa (p = 0.025 and p= 0.039). Conclusion: There is high prevalence of Helicobacter spp in patients with chronic cholecystitis and cholecystolithiasis without be correlated with the histological patterns studied.

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Hepatic Helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis

Cited by 445 publications

References 41 publications

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice

Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder

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Hepatic Helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis

Cited by 445 publications

References 41 publications

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10−/−mice

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10−/−mice

Identification of cdtB homologues and cytolethal distending toxin activity in enterohepatic Helicobacter spp.

Prevalence of Helicobacter spp in chronic cholecystitis and correlation with changes on the histological pattern of the gallbladder

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NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice

NovelHelicobacterspeciesH.japonicumisolated from laboratory mice from Japan induces typhlocolitis and lower bowel carcinoma in C57BL/129 IL10^−/−mice