Hepatic Insulin-Positive Cells and Major Transcription Factors (PDX1, MAFA, NGN3) in Rat Models of Type 1 and Type 2 Diabetes Mellitus

M, Baikenova; Черешнев, В. А.; Sokolova, Ksenia; Гетте, И. Ф.; Emelyanov, V. V.; Данилова, И. Г.

doi:10.1134/s0022093021040037

Cited by 2 publications

(4 citation statements)

References 27 publications

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“…These factors include PDX1, NGN3, and MAFA, which are crucial pancreatic βcell markers that play central role in the differentiation and maturation of various cell types into pancreatic β cells. [30][31][32] Li et al 33 reported that the cocktail containing transcriptional factors PDX1, MafA, and NGN3 differentiated acinar cells into β-like cells. PDX1 is an early embryonic marker for pancreas development expressed as early as embryonic Day 8.5 in mice and during the fourth week in humans.…”

Section: Discussionmentioning

confidence: 99%

“…We further quantified the transcriptional factors involved in pancreatic β cell differentiation and maturation. These factors include PDX1 , NGN3 , and MAFA , which are crucial pancreatic βcell markers that play central role in the differentiation and maturation of various cell types into pancreatic β cells 30–32 . Li et al 33 reported that the cocktail containing transcriptional factors PDX1 , MafA , and NGN3 differentiated acinar cells into β‐like cells.…”

Section: Discussionmentioning

confidence: 99%

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Effect of lawsone‐preconditioned mesenchymal stem cells on the regeneration of pancreatic β cells in Type 1 diabetic rats

Masnoon

Ishaque

Khan

et al. 2023

Cell Biochemistry & Function

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Diabetes is one of the major health issues globally. Type 1 diabetes mellitus develops due to the destruction of pancreatic β cells. Mesenchymal stem cells (MSCs) having remarkable self‐renewal and differentiation potential, can regenerate β cells. MSCs preconditioned with bioactive small molecules possess enhanced biological features and therapeutic potential under in vivo environment. Interestingly, compounds of naphthoquinone class possess antidiabetic and anti‐inflammatory properties, and can be explored as potential candidates for preconditioning MSCs. This study analyzed the effect of lawsone‐preconditioned human umbilical cord MSCs (hUMSCs) on the regeneration of β cells in the streptozotocin (STZ)‐induced Type 1 diabetes (T1D) rats. hUMSCs were isolated and characterized for the presence of surface markers. MSCs were preconditioned with optimized concentration of lawsone. T1D rat model was established by injecting 50 mg/kg of STZ intraperitoneally. Untreated and lawsone‐preconditioned hUMSCs were transplanted into the diabetic rats via tail vein. Fasting blood sugar and body weight were monitored regularly for 4 weeks. Pancreas was harvested and β cell regeneration was evaluated by hematoxylin and eosin staining, and gene expression analysis. Immunohistochemistry was also done to assess the insulin expression. Lawsone‐preconditioned hUMSCs showed better anti‐hyperglycemic effect in comparison with untreated hUMSCs. Histological analysis presented the regeneration of islets of Langerhans with upregulated expression of βcell genes and reduced expression of inflammatory markers. Immunohistochemistry revealed strong insulin expression in the preconditioned hUMSCs compared with the untreated hUMSCs. It is concluded from the present study that lawsone‐preconditioned hMSCs were able to exhibit pronounced anti‐hyperglycemic effect in vivo compared with hUMSCs alone.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of lawsone‐preconditioned mesenchymal stem cells on the regeneration of pancreatic β cells in Type 1 diabetic rats

Masnoon

Ishaque

Khan

et al. 2023

Cell Biochemistry & Function

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show abstract

“…Insulin+ cells found in various organs are attracting more and more attention of researchers, since they can partially compensate for damage of islet beta-cells in DM [ 6 , 7 ]. Presence of insulin+ cells in liver have been established both in healthy and diabetic animals [ 6 , 16 , 17 , 19 , 46 ]. Liver cells represent a potential target for conversion into insulin-producing cells for numerous reasons, including close origin from adjacent regions of the endoderm and relatedness of early embryonic stages with pancreas with expression of common regulatory expression factors [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Liver originated from the same region of the endoderm that pancreas and hepatic cells are able to transdifferentiate into pancreatic cells and vice versa [11][12][13][14][15]. In liver IPCs are detected as well in healthy [16], as in diabetic animals and/or under glucose load [7,8,[16][17][18][19], and researchers consider that the conversion of hepatocytes into insulin-producing cells could be the basis of a new type of therapy for diabetes. We aimed to identify factors, impact of generation of IPCs in liver in the rat models of T1D and T2D in vivo.…”

Section: Introductionmentioning

confidence: 99%

Hepatic insulin synthesis increases in rat models of diabetes mellitus type 1 and 2 differently

Abidov,

Sokolova,

Danilova

et al. 2023

PLoS ONE

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Insulin-positive (+) cells (IPCs), detected in multiple organs, are of great interest as a probable alternative to ameliorate pancreatic beta-cells dysfunction and insulin deficiency in diabetes. Liver is a potential source of IPCs due to it common embryological origin with pancreas. We previously demonstrated the presence of IPCs in the liver of healthy and diabetic rats, but detailed description and analysis of the factors, which potentially can induced ectopic hepatic expression of insulin in type 1 (T1D) and type 2 diabetes (T2D), were not performed. In present study we evaluate mass of hepatic IPCs in the rat models of T1D and T2D and discuss factors, which may stimulate it generation: glycaemia, organ injury, involving of hepatic stem/progenitor cell compartment, expression of transcription factors and inflammation. Quantity of IPCs in the liver was up by 1.7-fold in rats with T1D and 10-fold in T2D compared to non-diabetic (ND) rats. We concluded that ectopic hepatic expression of insulin gene is activated by combined action of a number of factors, with inflammation playing a decision role.

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Hepatic Insulin-Positive Cells and Major Transcription Factors (PDX1, MAFA, NGN3) in Rat Models of Type 1 and Type 2 Diabetes Mellitus

Cited by 2 publications

References 27 publications

Effect of lawsone‐preconditioned mesenchymal stem cells on the regeneration of pancreatic β cells in Type 1 diabetic rats

Effect of lawsone‐preconditioned mesenchymal stem cells on the regeneration of pancreatic β cells in Type 1 diabetic rats

Hepatic insulin synthesis increases in rat models of diabetes mellitus type 1 and 2 differently

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