Hepatic lipocytes: the principal collagen-producing cells of normal rat liver.

Friedman, Scott L.; Roll, F J; Boyles, J K; Bissell, D. Montgomery

doi:10.1073/pnas.82.24.8681

Cited by 772 publications

(524 citation statements)

References 31 publications

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“…8,[41][42][43] Immediately after purification, each cell type was recovered by centrifugation, lysed, and total cellular RNA was extracted and subjected to Northern analysis for interstitial collagenase, TIMP-1, and procollagen-1 mRNA as described above.…”

Section: Studies Of Timp-1 Mrna Expression In Hscs and Hepatocytes Exmentioning

confidence: 99%

Tissue Inhibitor of Metalloproteinase–1 Messenger Rna Expression Is Enhanced Relative to Interstitial Collagenase Messenger Rna in Experimental Liver Injury and Fibrosis

et al. 1996

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In liver fibrosis and cirrhosis there is a change in both the Liver fibrosis results from a relative imbalance beamount and relative composition of the hepatic extracellular tween synthesis and degradation of matrix proteins. We matrix. 1 Current evidence suggests that hepatic stellate cells have previously described release of the potent collagen-(lipocytes, fat-storing or Ito cells) are central to this process ase inhibitor, tissue inhibitor of metalloproteinase-1 both as the major source of fibrillar and nonfibrillar matrix (TIMP-1), by culture-activated human hepatic stellate proteins and matrix degrading metalloproteinases. 1-11 cells (HSCs). In this study, we have investigated the relaWe have previously shown that cultured hepatic stellate tive expression of TIMP-1 and interstitial collagenase in cells (HSCs) activated to a myofibroblastic phenotype express culture-activated rat HSCs and rat models of liver injury gelatinase A (72-kd type IV collagenase/gelatinase) 12,13 and and fibrosis. The complementary DNA (cDNA) for rat there is evidence to suggest that they also secrete stromely-TIMP-1 was obtained by homology polymerase chain reaction (PCR) and sequenced. By Northern analysis using sin. 14 A further metalloproteinase, interstitial collagenase, this probe, TIMP-1 messenger RNA (mRNA) expression expressed by mesenchymal and other cell types has degradawas up-regulated with HSC activation by culture on tive activity against native collagen types I and III, 15,16 explastic as defined by cellular expression of procollagen-pression of which could potentially initiate remodelling of 1. Interstitial collagenase mRNA was expressed in early fibrotic liver. culture (õ4 days) but became undetectable in more actiInterstitial collagenase expression in liver has been varivated cells (7-21 days). By activity assay of serum-free ously ascribed to parenchymal and sinusoidal liver cells, incell-conditioned media, TIMP-1 was found to be released cluding Kupffer cells 17,18 and hepatocytes. 19,20 There is also in increasing concentrations with duration of culture on some evidence to indicate that HSCs are a cellular source of plastic. Expression of TIMP-1, interstitial collagenase, interstitial collagenase in liver; studies on a passaged celland procollagen-1 mRNAs were studied in rat models of line derived by outgrowth from human liver (of possible HSC biliary and parenchymal injury (bile duct ligation and origin) showed that interstitial collagenase is expressed conCCl 4 administration) by ribonuclease protection assay. stitutively and is up-regulated in response to interleukin-TIMP-1 mRNA expression was increased at 6, 24 hours, 1 and tumor necrosis factor a. 21 Li et al. have also shown and 3 days after bile duct ligation and was also shown collagenase activity in cultured rat HSCs which was into rise in acute CCl 4 liver injury and remain elevated as creased in response to polyunsaturated lecithin. 22 the liver became fibrotic. TIMP-1 expression precededIn studies undertaken to measure interstitial collagenase proc...

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Section: Studies Of Timp-1 Mrna Expression In Hscs and Hepatocytes Exmentioning

confidence: 99%

Tissue Inhibitor of Metalloproteinase–1 Messenger Rna Expression Is Enhanced Relative to Interstitial Collagenase Messenger Rna in Experimental Liver Injury and Fibrosis

et al. 1996

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“…The cellular sources of the matrix molecules have been identified only partially. Whereas the formation of specific types of collagens (5 -18), fibronectin (19 -21), and of the basement membrane glycpprotein laminin (22) has been demonstrated by immunocytochemical and cell culture techniques in parenchymal (5 -14) and non-parenchymal (15)(16)(17)(18)(19)(20)(21)(22) cells, müch less is kiiown about the cell-type-specific fonnation of proteoglycans and hyaluronate. Recently, we demonstrated the ability of cultured fat storing cells to'produce suiphated proteoglycans (23) and hyaluronate (24).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Sulphated Glycosaminoglycan and Hyaluronate Synthesis and Secretion in Cultured Hepatocytes, Fat Storing Cells, and Kupffer Cells

Gressner¹,

Schäfer²

1989

Clinical Chemistry and Laboratory Medicine

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Summary:The extracellular matrix of normal liver contains several types of proteoglycans including heparan sulphate, chondroitin sulphate isomers, dermatan sulphate, and the glycosaminoglycan, hyaluronic acid. In the present study both the synthesis and secretion äs well äs the pattern of radioactively labeled proteoglycans and hyaluronic acid of hepatocytes, fat-storing cells (Ito cells), and Kupffer cells maintained in monolayer cultures under mostly identical conditions were compared to assess their relative contribution to hepatic proteoglycan synthesis. Fat-storing cells were identified äs the main type of cell producing and secreting proteoglycan and hyaluronic acid. More than 70% of labeled proteoglycan and hyaluronic acid were secreted into the medium. Heparan sulphate is the main type of proteoglycan in hepatocytes, whereas in the medium of fat-storing cells, chondroitin sulphate and dermatan sulphate comprise the mäjor fractions. Hyaluronic acid was not detectable in hepatocyte cultures and found only in low amounts in the medium of Kupffer cells. The results point to a stringent quantitative and qualitative cellular compartmentation of proteoglycan synthesis in liver with fat-storing cells äs the most important cell type for matrix proteoglycan and hyaluronic acid production.

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“…Many features of lipocyte activation in vivo are recapitulated by culture models (Friedman et al, 1985, Davis et al, 1987Gressner and Haarman, 1988;Geerts et al, 1989). Activation can be induced by the growth of lipocytes on uncoated plastic in the presence of serum.…”

Section: Role In Fibrogenesismentioning

confidence: 99%

“…Activation can be induced by the growth of lipocytes on uncoated plastic in the presence of serum. Like activation in vivo, culture-induced activation is accompanied by depletion of retinyl esters, cellular spreading, hypertrophy of rough endoplasmic reticulum, nuclear identation, and prominent thin filaments (De Leeuw et al, 1984;Friedman et al, 1985;Rockey et al, 1992). In addition to these morphologic features, culture-activated lipocytes also display functional properties akin to their in vivo counterparts.…”

Section: Role In Fibrogenesismentioning

confidence: 99%

“…For example, culture-activated cells proliferate during activation on uncoated plastic (Gressner and Haarman, 1988;Davis et al, 1990;Friedman et al, 1989). They also produce increased amounts of total collagen, specific collagen types (i.e., I), laminin, fibronectin, and proteoglycans (Friedman et al, 1985;Schafer et al, 1987;Ramadori et al, 1987;Maher et al, 1988;Weiner et al, 1990).…”

Section: Role In Fibrogenesismentioning

confidence: 99%

See 1 more Smart Citation

Cytoskeleton of liver perisinusoidal cells (lipocytes) in normal and pathological conditions

Rockey

Friedman

1992

Cell Motil. Cytoskeleton

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Hepatic lipocytes: the principal collagen-producing cells of normal rat liver.

Cited by 772 publications

References 31 publications

Tissue Inhibitor of Metalloproteinase–1 Messenger Rna Expression Is Enhanced Relative to Interstitial Collagenase Messenger Rna in Experimental Liver Injury and Fibrosis

Tissue Inhibitor of Metalloproteinase–1 Messenger Rna Expression Is Enhanced Relative to Interstitial Collagenase Messenger Rna in Experimental Liver Injury and Fibrosis

Comparison of Sulphated Glycosaminoglycan and Hyaluronate Synthesis and Secretion in Cultured Hepatocytes, Fat Storing Cells, and Kupffer Cells

Cytoskeleton of liver perisinusoidal cells (lipocytes) in normal and pathological conditions

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