Hepatic phase of malaria is the target of cellular mechanisms induced by the previous and the subsequent stages. A crucial role for liver nonparenchymal cells

Mazier, Dominique; Rénia, Laurent; Nüssler, Andreas K.; Pied, Sylviane; Marussig, Myriam; Goma, Joseph; Grillot, Didier; Miltgen, F; Drapier, Jean‐Claude; Corradin, Giampietro; Giudice, Giuseppe Del; Grau, Georges E.

doi:10.1016/0165-2478(90)90093-6

Cited by 33 publications

(15 citation statements)

References 37 publications

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“…It was reported earlier that IFN-7 and TNF inhibit the development of plasmodial liver stages in vitro (14,20). In vivo treatment of sporozoite-immunized A/J mice with anti-IFN-7 monoclonal antibody was reported to reverse protection against P. berghei sporozoites (15).…”

Section: Discussionmentioning

confidence: 99%

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

Rodrigues¹,

Cordey²,

Arreaza³

et al. 1991

Int Immunol

207

179

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Section: Discussionmentioning

confidence: 99%

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

Rodrigues¹,

Cordey²,

Arreaza³

et al. 1991

Int Immunol

207

179

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“…In the case of immunity to pre-erythrocytic stages, there are data to support both direct and cell-mediated effects of Ab, with isotype distribution potentially being critical for the latter [77, 781. Indeed, in F1 berghei CS-immunized mice, it was found that titers of mouse (cytophilic) IgGl to the Spz surface and Ab avidity paralleled acquisition of sterile immunity [77]. Also, infected hepatocytes have been found to be prone to Kupffer cell-mediated ADCC [78]. As a result of its expression by intrahepatocytic parasites [25], STARP may be a potential target of ADCC.…”

Section: Isotype Analysis Of Humoral Responses To the Starp Agmentioning

confidence: 97%

Plasmodium falciparum sporozoite invasion is inhibited by naturally acquired or experimentally induced polyclonal antibodies to the STARP antigen

et al. 1997

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Antibody(Ab)-mediated inhibition of sporozoite invasion of hepatocytes is a mechanism that has been clearly demonstrated to act upon Plasmodium falciparum pre-erythrocytic stages in humans. Consequently we have analyzed the Ab response to a recently identified P. falciparum sporozoite surface protein, STARP, in malaria-exposed individuals and tested the inhibitory effect of these Ab upon hepatocyte invasion in vitro. STARP-specific IgG were detected in 90 and 61% of sera from regions where individuals were exposed to 100 and 1-5 infectious bites per year, respectively. These IgG were predominantly of the cytophilic IgG1 or IgG3 type. STARP and the major sporozoite surface protein, CS, elicited equivalent IgG levels in adults. When affinity purified from either African immune sera or the serum of an individual experimentally protected by irradiated sporozoite immunization, STARP-specific Ab prevented up to 90% of sporozoites from invading human hepatocytes. The dose-dependent and reproducible inhibition was more pronounced than that observed with human CS-specific Ab affinity purified under identical conditions. Substantial reduction of sporozoite invasion was also observed with Ab induced by artificial immunization with recombinant STARP protein and reactive with the native protein. Taken together with recent findings of human cytotoxic T lymphocytes specific for this antigen, these results promote the interest of studying the efficacy of STARP as a target for immune effector mechanisms operating upon pre-erythrocytic stages.

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“…This is an important observation since the poor performance seen in the past with repeat-unit-based vaccines has largely been attributed to inadequate T-cell help (Del Guidice et al, 1990). To produce a highly effective subunit vaccine, however, it remains important to include an appropriate T-cell determinant so that both Th-and Bcell compartments can be effectively engaged, and antibodies of the desired speci® city be eventually produced (Celada and Sercarz, 1988).…”

Section: Discussionmentioning

confidence: 98%

“…Both the sporozoites and the erythrocytic stages of malarial parasites can modulate the hepatic phase via cytokines, notably interferon-c (IFN-c ), tumour necrosis factor (TNF) and interleukin-6 (IL-6), directly, as a result of a cascade of events, or by MHCrestricted or antibody-dependent cellmediated cytotoxicity (Mazier et al, 1990). IFN-c has both a parasitostatic effect and a lytic effect on the developing liver-stage parasites and indirectly promotes the activation of effector T-cells (Krzych et al, 2000).…”

mentioning

confidence: 99%

Inducing a cell-mediated immune response against peptides of the Plasmodium vivax circumsporozoite protein

Thomas

Koduri²,

Chopra³

et al. 2001

Annals of Tropical Medicine And Parasitology

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An attempt was made to generate an unrestricted cellular immune response against peptide antigens of the circumsporozoite protein (CSP) of Plasmodium vivax. The peptides used represent the repeat-region sequences of the type-I variant (AA and DA) or type-II variant (ANG) and the conserved region (region II) containing the hepatocyte-binding region extended to include a T-cell epitope (HBP). The study was conducted in outbred mice and two genetically unrelated inbred strains of mice. Spleen cells, recovered from mice that had been primed either with one peptide or a conjugate formed of HBP linked to one of the repeat-region peptides, were pulsed in vitro with varying amounts of individual peptides/conjugates, both in soluble and particulate form (with and without a human beta-casein bio-active fragment analogue as adjuvant). In the tests using the cells from the mice primed with an individual peptide(s), HBP showed a high proliferation index, and the repeat-region peptides, especially AA, showed T-cell activity in at least one of the mouse strains studied. In vitro, higher concentrations of the free peptides than of liposomal preparations of the peptides had to be used to elicit the optimal proliferation of the cells from each strain of mice. Interestingly, the cells from the conjugate-primed mice showed enhanced proliferation (compared with that observed in the cells from mice primed with individual peptides) when stimulated with each component, and especially the repeat-region sequence, of the relevant conjugate. In such cases there was no evidence of restriction of the immune response by the major histocompatibility complex. The major secreted cytokines were found to be from CD4(+) Th1 (interferon-gamma and interleukin-2), with relatively low levels of the Th-2 cytokines interleukin-4 and interleukin-6. The delivery of cohered 'B-T' peptide(s) sequences from the same protein, ideally with an immunostimulatory adjuvant or as a liposomal preparation, should greatly enhance the cell-mediated immune response and should improve clearance of mosquito-inoculated P. vivax sporozoites.

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Hepatic phase of malaria is the target of cellular mechanisms induced by the previous and the subsequent stages. A crucial role for liver nonparenchymal cells

Cited by 33 publications

References 37 publications

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

Plasmodium falciparum sporozoite invasion is inhibited by naturally acquired or experimentally induced polyclonal antibodies to the STARP antigen

Inducing a cell-mediated immune response against peptides of the Plasmodium vivax circumsporozoite protein

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Hepatic phase of malaria is the target of cellular mechanisms induced by the previous and the subsequent stages. A crucial role for liver nonparenchymal cells

Cited by 33 publications

References 37 publications

CD8+ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

CD8+ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

Plasmodium falciparum sporozoite invasion is inhibited by naturally acquired or experimentally induced polyclonal antibodies to the STARP antigen

Inducing a cell-mediated immune response against peptides of the Plasmodium vivax circumsporozoite protein

Contact Info

Product

Resources

About

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

CD8⁺ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria