Hepatic Response to the Interaction Between Thymoquinone and Iron-Dextran: an In Vitro and In Vivo Study

Ghasemi, Farzad; Ghaffari, Fatemeh; Omidifar, Navid; Azandaryani, Masoumeh Taheri; Nili‐Ahmadabadi, Amir

doi:10.1007/s12011-022-03249-9

Cited by 4 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liver is the hub of metabolic processes and plays a vital role in detoxifying waste metabolites [ 35 ]. Ghasemi et al discovered that iron overload decreases the activity of antioxidant enzymes in the liver, causing the liver to create a significant concentration of ROS and impairing liver function [ 36 ]. Vetuschi et al showed that iron overload could antagonize ferroptosis through negative feedback regulation of hepatic release of glutathione peroxidase 4 [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Melatonin Repairs Osteoporotic Bone Defects in Iron-Overloaded Rats through PI3K/AKT/GSK-3β/P70S6k Signaling Pathway

Ren

Liu

Jiang

et al. 2023

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

It was found recently that iron overload can cause osteoporosis in rats. Through in vitro and in vivo experimentations, the purpose of the present study was to validate and confirm the inhibitory effects of melatonin on iron death of osteoporosis and its role in bone microstructure improvements. Melatonin (100 mol/L) was administered to MC3T3-E1 cells induced by iron overload in vitro for 48 hours. The expression of cleaved caspase-3 and cleaved PARP and the production of ROS (reactive oxygen species) and mitochondrial damage were all exacerbated by iron overload. On the other hand, melatonin restored these impacts in MC3T3-E1 cells produced by iron overload. By evaluating the expression of PI3K/AKT/GSK-3β/P70S6k signaling pathway-related proteins (RUNX2, BMP2, ALP, and OCN) using RT-PCR and Western blot, osteogenic-related proteins were identified. Alizarin red S and alkaline phosphatase were utilized to evaluate the osteogenic potential of MC3T3-E1 cells. Melatonin significantly improved the osteogenic ability and phosphorylation rates of PI3K, AKT, GSK-3β, and P70S6k in iron overload-induced MC3T3-E1 cells. In vivo, melatonin treated iron overload-induced osteoporotic bone defect in rats. Rat skeletal microstructure was observed using micro-CT and bone tissue pathological section staining. ELISA was utilized to identify OCN, PINP, CTX-I, and SI in the serum of rats. We discovered that melatonin increased bone trabecular regeneration and repair in osteoporotic bone defects caused by iron overload. In conclusion, melatonin enhanced the osteogenic ability of iron overload-induced MC3T3-E1 cells by activating the PI3K/AKT/GSK-3β/P70S6k signaling pathway and promoting the healing of iron overload-induced osteoporotic bone defects in rats.

show abstract

Section: Discussionmentioning

confidence: 99%

Melatonin Repairs Osteoporotic Bone Defects in Iron-Overloaded Rats through PI3K/AKT/GSK-3β/P70S6k Signaling Pathway

Ren

Liu

Jiang

et al. 2023

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…In line with that, TQ showed in-vitro ferrous (Fe2+) concentration-dependent chelation activity [ 17 ]. Conversely, TQ intervention showed no direct in-vivo iron chelation activity on ferrous iron (Fe2+) [ 19 ]. Instead, TQ showed an antioxidant effect against iron overload-induced oxidative stress [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…Earlier studies have reported the iron chelation activity and several therapeutic effects of nigella sativa [ 17 ], precisely the cardio protection, antioxidant, Fe3+ and Fe2+ iron chelation, Hepato-protective, immunomodulatory [ [17] , [18] , [19] , [20] ]. Thymoquinone (TQ) is the principal component of nigella sativa that demonstrates all of those therapeutic activities [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Manuka combinations with nigella sativa and hydroxyurea in treating iron overload of pediatric β-thalassemia major, randomized clinical trial

Gamaleldin,

Abraham,

Meabed

et al. 2024

Heliyon

View full text Add to dashboard Cite

“…Then, the CAT enzyme metabolizes hydrogen peroxide into water molecules and molecular oxygen (Nandi et al, 2019). The interaction of natural compounds with SOD/CAT enzymes can play an important role in toxicity of reactive oxygen species (Ghasemi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

The Cytotoxic Effect of Thymoquinone Enhance on HepG2 Cell Line due to Induction of Fenton Reaction by Hydrogen Peroxide: An In Vitro and In Silico Study

Ghelichkhani,

Saffari-Chaleshtori,

Ghaffari

et al. 2023

Asian Pac J Cancer Prev

View full text Add to dashboard Cite

Objective: Thymoquinone (TQ) is a component derived from the volatile oil of Nigella sativa. Fenton reaction induction is a well-known strategy to prevent the growth of cancer cells which can stimulate by hydrogen peroxide. This study was designed to investigate the TQ effects on hydrogen peroxide-induced cytotoxicity. Methods: In this study, HepG2 cell survival, reactive oxygen species (ROS) production, cell membrane integrity, and changes of superoxide dismutase (SOD)/ catalase (CAT) activity were evaluated following incubation of HepG2 cells with 31 μM hydrogen peroxide and different concentrations of TQ (18.5, 37 and 75 μM). In addition, molecular docking studies on the interference of TQ with CAT/SOD enzymes were investigated. Results: Our findings showed that TQ low concentration can increase the survival of HepG2 cells when exposed to hydrogen peroxide, and on the contrary, its high concentration can potentiate cytotoxicity induced by hydrogen peroxide. The TQ alongside hydrogen peroxide increased the production of ROS, which was related to increase CAT and SOD activity in the HepG2 cells. Molecular docking findings showed that TQ effects on the formation of free radicals were not related to its chemical interference with the structure of the SOD/CAT molecules. Conclusion: Fenton reaction induction may increase the effectiveness of TQ in preventing HepG2 cells proliferation.

show abstract

Hepatic Response to the Interaction Between Thymoquinone and Iron-Dextran: an In Vitro and In Vivo Study

Cited by 4 publications

References 44 publications

Melatonin Repairs Osteoporotic Bone Defects in Iron-Overloaded Rats through PI3K/AKT/GSK-3β/P70S6k Signaling Pathway

Melatonin Repairs Osteoporotic Bone Defects in Iron-Overloaded Rats through PI3K/AKT/GSK-3β/P70S6k Signaling Pathway

Manuka combinations with nigella sativa and hydroxyurea in treating iron overload of pediatric β-thalassemia major, randomized clinical trial

The Cytotoxic Effect of Thymoquinone Enhance on HepG2 Cell Line due to Induction of Fenton Reaction by Hydrogen Peroxide: An In Vitro and In Silico Study

Contact Info

Product

Resources

About