Hepatic Stem Cell Compartment: Activation and Lineage Commitment

The Promoter of Human Telomerase Reverse Transcriptase Is Activated During Liver Regeneration and Hepatocyte Proliferation

“…In the differentiated (non-proliferating) state, these cells exhibit features of primary human hepatocytes. 20 When seeded at low density (1x10 5 cells per well into 12-well plates), HepaRG cells proliferate up to 5-6 days post-plating ceasing proliferation from thereon ( Fig. 2A) accompanied by the increase of hepatocytespecific markers pre-albumin and APOA1 during the differentiation of HepaRG cells (Fig.…”

“…The liver contains bipotent stem and progenitor cells. 5 However, differentiated hepatocytes contribute to liver regeneration in response to injury and labelling of regenerating liver cells in mice revealed that 80-90% of the liver cells participate in regeneration. 6 Activation of telomerase could contribute to the maintenance of regenerative reserve in adult human liver.…”

2-acetylaminofluorene dose-dependent differentiation of rat oval cells into hepatocytes: Confocal and electron microscopic studies

“…38 One of the major differences between the intestinal glands and hepatocytes is that the enterocytes reside on a laminin-containing basement membrane. It is possible that the upregulation of HNF-4 without the disintegration of the basement membrane may be responsible for the "abnormal" differentiation of oval cells into intestinal epithelium.…”

Hepatic oval cell activation in response to injury following chemically induced periportal or pericentral damage in rats

“…[3][4][5][6][7] Monoclonal antibodies such as OV6, OC2, and BD1 also aid in their characterization. 8,9 High levels of certain mRNAs like AFP and stem cell factor can also be expressed by oval cells. 10 There are several previous studies that have documented the emergence of oval cells following chemical injury, such as with ethionine 11 and, more recently, with galactosamine.…”