Hepatic trematode Platynosomum sp. (Dicrocoeliidae) from a domestic cat in Colombo, Sri Lanka: Case report and molecular identification

Ranaraja, Dissanayaka Mudiyanselage Asha Erandhi; Mahakapuge, Thilini Anupama Nanayakkarawasam; Thilakarathne, Dulari S.; Dharmasuriya, Juliyabaduge Yushika Chamodi; Rajapakse, Rajapakse Peramune Veddikkarage Jayanthe

doi:10.1016/j.vprsr.2022.100762

Cited by 4 publications

(6 citation statements)

References 28 publications

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“…Platynosomum infection represents a significant concern in cat populations, with documented prevalence rates varying from 0.07% to 9.76% in Asia [3][4][5][6][7][8][9][10]. In Thailand,…”

Section: Discussionmentioning

confidence: 99%

“…Available sequences of Platynosomum spp. from Vietnam [6], Brazil [18], and Sri Lanka [4] were used as reference sequences for tree construction. In addition, a sequence of Dipylidium caninum from Italy (MT806359) [25] and Dicrocoelium dendriticum from Japan (LC629058) [26] were used as an outgroup species for the cox1 gene and ITS2 region, respectively.…”

Section: Molecular Technique For Species Identificationmentioning

confidence: 99%

“…The prevalence of this liver fluke has been reported to range from 0.07% to 81% worldwide [2]. In Asian countries, liver fluke infection in cats has been reported in Malaysia [3], Sri Lanka [4], Vietnam [5,6], Thailand [7][8][9], and Korea [10].…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes

Chantawong,

Potiwong,

Choochote

et al. 2024

Animals

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Platynosomum spp., a hepatic trematode, causes fatal hepatobiliary disease in cats. Feline platynosomiasis is often underestimated due to a lack of awareness and diagnostic challenges. This study aimed to investigate the occurrence, factors, and clinicopathological abnormalities associated with Platynosomum spp. infection in cats with elevated serum ALT levels. Platynosomum infection was determined using zinc sulfate flotation and formalin–ether sedimentation. DNA sequence analysis of PCR products from the Platynosomum internal transcribed spacer 2 (ITS2) region and cox1 gene was used to identify Platynosomum species. Of a total of 43 cat fecal samples, the proportion of Platynosomum spp. infection by microscopic examination was 11.63% (5/43). All PCR-positive samples were molecularly identified as Platynosomum illiciens. From the logistic regression analysis, the odds of Platynosomum infection in cats without a deworming program were 16 times higher than those of regularly dewormed cats. Demographic data, housing conditions, and predatory behavior were not significantly associated with the infection. Regarding blood profiles, infected cats had higher eosinophil counts (p = 0.014), with no significant differences in ALT (p = 0.791) or ALP (p = 0.970) levels compared to non-infected cats. Our findings demonstrate that eosinophilia in cats with increased serum ALT may suggest P. illiciens infection in endemic areas. We strongly recommend a regular deworming program to mitigate the risk of P. illiciens infection.

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“…Platynosomum infection represents a significant concern in cat populations, with documented prevalence rates varying from 0.07% to 9.76% in Asia [3][4][5][6][7][8][9][10]. In Thailand,…”

Section: Discussionmentioning

confidence: 99%

Section: Molecular Technique For Species Identificationmentioning

confidence: 99%

See 1 more Smart Citation

Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes

Chantawong,

Potiwong,

Choochote

et al. 2024

Animals

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“…In the definitive hosts, which are generally asymptomatic, adult trematodes localize in the choledochus and gall bladder [ 224 ]. While most papers reporting this parasitism in different parts of the world did not describe cancerogenesis [ 222 , 225 , 226 ], seven cases of cholangiocarcinomas in adult cats parasitized by P. illiciens were reported in Brazil [ 227 , 228 ]. The liver tumors were composed of atypical neoplastic cells arranged in acini, and metastases to various organs were noted, with visible pre-neoplastic alterations resembling proliferative or chronic inflammatory lesions seen in other trematode-induced malignancies [ 21 ].…”

Section: Parasites Associated With Neoplasia In Domestic and Wild Ani...mentioning

confidence: 99%

Cancerogenic parasites in veterinary medicine: a narrative literature review

Fonti

Parisi

Mancianti

et al. 2023

Infect Agents Cancer

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Parasite infection is one of the many environmental factors that can significantly contribute to carcinogenesis and is already known to be associated with a variety of malignancies in both human and veterinary medicine. However, the actual number of cancerogenic parasites and their relationship to tumor development is far from being fully understood, especially in veterinary medicine. Thus, the aim of this review is to investigate parasite-related cancers in domestic and wild animals and their burden in veterinary oncology. Spontaneous neoplasia with ascertained or putative parasite etiology in domestic and wild animals will be reviewed, and the multifarious mechanisms of protozoan and metazoan cancer induction will be discussed.

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“…Hence, the infection's outcome can range from asymptomatic to severe and lethal infections. In the case of chronic and/or heavy infection, P. fastosum can induce mottled liver, gallbladder distension, hepatitis, cholangiohepatitis, cholecystitis, periductal fibrosis, biliary cirrhosis, and biliary obstruction (Basu and Charles 2014;Ramos et al 2016;Nur-Amalina et al 2022). Besides, P. fastosum infection has been found to be associated with cholangiocarcinoma (Andrade et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Morphology and Ultrastructural Characteristics of Platynosomum fastosum Kossack, 1910 Adults and Eggs

2023

Int J Vet Sci

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Infection with Platynosomum fastosum, a liver fluke of domestic cats, can induce biliary obstruction and has been found to be associated with cholangiocarcinoma. To evaluate any new rational drugs, fundamental knowledges of parasite morphology are crucial but detailed morphology has not yet been investigated. Macroscopic, microscopic, and ultrastructural morphology of P. fastosum adult worms and eggs were evaluated. The adult fluke was reddish brown with a tamarind leaf-like body shape, 4.5mm in length and 1.5mm in width. Under scanning electron microscope (SEM), the tegumental surface of P. fastosum was spineless and covered with slender villous-like projections. Four types of papillae were found, and all were non-ciliated. P. fastosum fluke eggs were dark brown with an operculum and had a smooth eggshell surface with an inconspicuous posterior abopercular knob. From both fecal and bile samples, at least 3 types of eggs were present. The average size (mean±SD) of a typical P. fastosum egg was 44.2+1.7m in length and 31.8+2.2m in width. Under transmission electron microscope (TEM), tegument syncytium was composed of three layers. The first layer was an outermost trilaminate membrane covered by a glycocalyx. The second layer was tegumental cytoplasm composed of 2 types of tegumental granules, lysosomes, mitochondria and microtrabeculae. The third layer attached to a basal extracellular matrix with numerous basal membrane infoldings. This study revealed the first ultrastructural characteristics of P. fastosum adult and egg as a fundamental knowledge to use as basic criteria for assessment of anti-parasitic drug development in the future.

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Hepatic trematode Platynosomum sp. (Dicrocoeliidae) from a domestic cat in Colombo, Sri Lanka: Case report and molecular identification

Cited by 4 publications

References 28 publications

Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes

Occurrence and Risk Factors Associated with Platynosomum illiciens Infection in Cats with Elevated Liver Enzymes

Cancerogenic parasites in veterinary medicine: a narrative literature review

Morphology and Ultrastructural Characteristics of Platynosomum fastosum Kossack, 1910 Adults and Eggs

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